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Molecluar ecological analysis of nitrogen fixation mechanism in the symbiotic microbial community of sweet potato

Research Project

Project/Area Number 15H04620
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental agriculture(including landscape science)
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

Ikeda Seishi  国立研究開発法人農業・食品産業技術総合研究機構, 北海道農業研究センター 大規模畑作研究領域, 上級研究員 (20396609)

Co-Investigator(Kenkyū-buntansha) 小林 有紀  国立研究開発法人農業・食品産業技術総合研究機構, 九州沖縄農業研究センター 畑作研究領域, 上級研究員 (00729519)
小林 晃  国立研究開発法人農業・食品産業技術総合研究機構, 九州沖縄農業研究センター 畑作研究領域, 上級研究員 (90626954)
関山 恭代  国立研究開発法人農業・食品産業技術総合研究機構, 食品研究部門 食品分析研究領域, 上級研究員 (60342804)
菊地 淳  国立研究開発法人理化学研究所, 環境資源科学研究センター, チームリーダー (00321753)
鶴丸 博人  鹿児島大学, 農水産獣医学域農学系, 助教 (60545226)
塔野岡 純子  佐賀大学, 農学部, 特定研究員 (00713314)
Co-Investigator(Renkei-kenkyūsha) HIRAFUJI Masayuki  東京大学, 大学院農学生命科学研究科, 特任教授 (00370495)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Keywords窒素固定 / サツマイモ / 共生細菌 / 多様性 / 16S rRNA遺伝子 / Alphaproteobacteria / メタノール培地 / Rhizobiales / Rhodopseudomonas / 微生物生態 / 共生 / NMR / メタボローム解析 / 微生物 / 細菌 / 応用微生物 / 環境 / 生態学 / 微生物多様性
Outline of Final Research Achievements

Bacterial diversity in stem, surface and inner tissues of main root of sweet potato was examined by non-cultivation method. As a result, the domination of Alphaproteobacteria was observed in all tissues examined in comparison with bacterial diversities in other crops. As expected, the values of diversity indexes are decreased as folloing order, surface of main root, stem, and inner tissue of main root. Correspondingly, approximately 2000 bacterial colonies were isolated from stem, surface and inner tissues of main root of sweet potato by using a culivation medium. Approximately 320 groups were obtained by the clustering analysis based on 16S rRNA gene. These isolates were examined for nitrigen fixation ability on a medium. Among the isolates, one isolate closely related to Bradyrhizobium sp. was found to have nitrogen fixation ability as a candidate of nirogen fixation bacterium in sweet potato.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (9 results)

All 2017 2016 2015

All Journal Article (6 results) (of which Peer Reviewed: 5 results,  Acknowledgement Compliant: 2 results) Presentation (2 results) (of which Invited: 2 results) Book (1 results)

  • [Journal Article] Proposal for Practical Application of Field Metabolomics2017

    • Author(s)
      関山恭代、池田成志、冨田理
    • Journal Title

      KAGAKU TO SEIBUTSU

      Volume: 55 Issue: 6 Pages: 392-399

    • DOI

      10.1271/kagakutoseibutsu.55.392

    • NAID

      130006742954

    • ISSN
      0453-073X, 1883-6852
    • Year and Date
      2017-05-20
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 植物共生科学から考える土壌病害の発生しにくい土づくり2017

    • Author(s)
      池田成志
    • Journal Title

      土づくりとエコ農業

      Volume: 49 Pages: 33-39

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Rice bran amendment suppresses potato common scab by increasing the populations of antagonistic bacteria in rhizosphere2016

    • Author(s)
      Tsuyoshi Tomihama, Yatsuka Nishi, Kiyofumi Mori, Toshiya Iida, Shihomi Uzuhashi, Moriya Ohkuma, and Seishi Ikeda
    • Journal Title

      Phytopathology

      Volume: In press Issue: 7 Pages: 719-728

    • DOI

      10.1094/phyto-12-15-0322-r

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 土壌微生物が創る共生の世界―その先端的研究事例と農業への応用的研究展開5.作物共生微生物の生態研究の現状と農業・食品産業への応用の可能性2016

    • Author(s)
      池田成志
    • Journal Title

      日本土壌肥料学雑誌

      Volume: 87 Pages: 373-382

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 植物共生細菌群集を利用した持続的農業2016

    • Author(s)
      大久保卓、池田成志、南澤究
    • Journal Title

      土と微生物

      Volume: 70 Pages: 10-16

    • NAID

      130005170826

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Community analysis of root- and tuber-associated bacteria in field-grown potato plants harboring different resistance levels against common scab2015

    • Author(s)
      A. Kobayashi, Y. O. Kobayashi, N. Someya, and S. Ikeda
    • Journal Title

      Microbes and Environments

      Volume: 30 Pages: 301-309

    • NAID

      130005116039

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Presentation] 農業・食品分野における微生物科学とメタボロミクスの連携の可能性について2017

    • Author(s)
      池田成志、関山恭代
    • Organizer
      第11回メタボロームシンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 共生微生物学の視点から考える理想の土づくりと農業・食品産業の新展開2016

    • Author(s)
      池田成志
    • Organizer
      北海道d上肥料懇話会・日本土壌肥料学会北海道支部会 共催
    • Place of Presentation
      とかちプラザ(北海道・帯広市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Book] メタゲノム解析実験プロトコール(植物共生細菌群集のメタゲノム解析)2016

    • Author(s)
      池田成志、大久保卓、鶴丸博人、南澤究(分担執筆、監修:服部正平)
    • Total Pages
      229
    • Publisher
      羊土社
    • Related Report
      2016 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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