Analysis of physiological roles of the Plant post-Golgi organelle in stress response.

• Project/Area Number: 15H04627
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Ochanomizu University (2017-2018); The University of Tokyo (2015-2016)
• Principal Investigator: Uemura Tomohiro お茶の水女子大学, 理系女性教育開発共同機構, 准教授 (90415092)
• Research Collaborator: Nakano Ryohei Thomas
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000); Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
• Keywords: 植物 / トランスゴルジ網 / うどんこ病 / 塩ストレス / ライブイメージング / TGN / SNARE / オルガネラ / 病原菌応答 / 膜交通 / 細胞 / ストレス

Outline of Final Research Achievements

Based on our observation, we found that the GA-TGN gradually matures into the GI-TGN by acquiring components of the secretory pathway, and GI-TGN is required for pathogen-inducible protein secretion. In addition, quantitative proteomic approach demonstrated that the SYP4-VAMP721 secretory pathway mediates secretion of cell wall modification enzymes and is important for plant growth and extracellular defense against powdery mildew pathogens. Furthermore, we established long-time observation system under salt stress response.

Academic Significance and Societal Importance of the Research Achievements

トランスゴルジ網は細胞内タンパク質輸送の中心に位置する分岐点となる重要な区画であり、その機能がなくなると植物が病原菌に感染しやすくなることを発見した。トランゴルジ網の機能を制御することで、病気に強い植物の作出や、農薬の開発が期待される。

Research Products

• [Int'l Joint Research] Max Planck Institute(ドイツ)
• [Int'l Joint Research] マックスプランク研究所(ドイツ)
• [Journal Article] A Missense Mutation in the <i>NSF</i> Gene Causes Abnormal Golgi Morphology in <i>Arabidopsis thaliana</i> (2018)
  • Author(s): Tanabashi Sayuri、Shoda Keiko、Saito Chieko、Sakamoto Tomoaki、Kurata Tetsuya、Uemura Tomohiro、Nakano Akihiko
  • Journal Title: Cell Structure and Function Volume: 43 Issue: 1 Pages: 41-51
  • DOI: 10.1247/csf.17026
  • NAID: 130006565491
  • ISSN: 0386-7196, 1347-3700
  • Peer Reviewed / Open Access
• [Journal Article] Golgi entry core compartment functions as the COPII-independent scaffold for ER-Golgi transport in plant cells (2017)
  • Author(s): Ito Yoko、Uemura Tomohiro、Nakano Akihiko
  • Journal Title: Journal of Cell Science Volume: 131 Issue: 2
  • DOI: 10.1242/jcs.203893
  • Peer Reviewed
• [Journal Article] Evolutionarily conserved BIL4 suppresses the degradation of brassinosteroid receptor BRI1 and regulates cell elongation (2017)
  • Author(s): Yamagami Ayumi、Saito Chieko、Nakazawa Miki、Fujioka Shozo、Uemura Tomohiro、Matsui Minami、Sakuta Masaaki、Shinozaki Kazuo、Osada Hiroyuki、Nakano Akihiko、Asami Tadao、Nakano Takeshi
  • Journal Title: Sci. Rep. Volume: 7 Issue: 1 Pages: 5739-5739
  • DOI: 10.1038/s41598-017-06016-2
  • Peer Reviewed / Open Access
• [Journal Article] Cadmium-induced changes in vacuolar aspects of Arabidopsis thaliana. (2017)
  • Author(s): Shanti S. Sharma, Kotaro Yamamoto, Kohei Hamaji, Miwa Ohnishi, Aya Anegawa, Shashi Sharma, Sveta Thakur, Vijay Kumar, Tomohiro Uemura, Akihiko Nakano, Tetsuro Mimura
  • Journal Title: Plant Physiol Biochem. Volume: 114 Pages: 29-37
  • DOI: 10.1016/j.plaphy.2017.02.017
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The trans-Golgi network and the Golgi stacks behave independently during regeneration after Brefeldin A treatment in tobacco BY-2 cells (2017)
  • Author(s): Ito, Y., Toyooka, K., Fujimoto, M., Ueda, T., Uemura, T. and Nakano A.
  • Journal Title: Plant Cell Phys. Volume: 印刷中 Issue: 4 Pages: 811-821
  • DOI: 10.1093/pcp/pcx028
  • Peer Reviewed / Open Access
• [Journal Article] Distribution of RAB5-positive multivesicular endosomes and the <i>trans</i>-Golgi network in root meristematic cells of <i>Arabidopsis thaliana</i> (2016)
  • Author(s): Ito, E., Uemura, T., Ueda, T. (corresponding author), and Nakano, A.
  • Journal Title: Plant Biotechnology Volume: 33 Issue: 4 Pages: 281-286
  • DOI: 10.5511/plantbiotechnology.16.0218a
  • NAID: 130005281074
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Physiological Roles of Plant Post-Golgi Transport Pathways in Membrane Trafficking (2016)
  • Author(s): Uemura, T.
  • Journal Title: Plant Cell Physiol. Volume: 57 Issue: 10 Pages: 2013-2019
  • DOI: 10.1093/pcp/pcw149
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] SNARE Molecules in Marchantia polymorpha: Unique and Conserved Features of the Membrane Fusion Machinery. (2016)
  • Author(s): Kanazawa, T., Era, A., Minamino, N., Shikano, Y., Fujimoto, M., Uemura, T., Nishihama, R., Yamato, KT., Ishizaki, K., Nishiyama, T., Kohchi, T., Nakano, A., Ueda, T.
  • Journal Title: Plant Cell Physiol. Volume: 57 Issue: 2 Pages: 307-24
  • DOI: 10.1093/pcp/pcv076
  • Peer Reviewed / Open Access
• [Presentation] Functional zones and dynamics of trans-Golgi network in Arabidopsis (2017)
  • Author(s): Yutaro Shimizu, Yamato Komatsu, Yoko Ito, Junpei Takagi J, Kazuo Ebine, Takashi Ueda, Kazuo Kurokawa, Tomohiro Uemura, Akihiko Nakano.
  • Organizer: 20th European Network for Plant Endomembrane Research meeting
  • Int'l Joint Research
• [Presentation] 超解像ライブメージング観察による植物オルガネの動態と構造 (2017)
  • Author(s): 植村知博
  • Organizer: 異分野融合ワークショップ『細胞外から受ける情報のイメージングとそれを支える解析技術の最前線』
• [Presentation] The dynamics and physiological roles of the TGN in plants. (2016)
  • Author(s): Tomohiro Uemura
  • Organizer: Progress100: Second international symposium: Protein Trafficking and Intracellular Signaling of Plant and Fungal Cells
  • Place of Presentation: 九州大学 （福岡県博多市）
  • Year and Date: 2016-02-08
  • Int'l Joint Research / Invited
• [Presentation] The dynamics of trans-Golgi network (TGN) in plants (2015)
  • Author(s): Tomohiro Uemura, Takashi Ueda, and Akihiko Nakano.
  • Organizer: 26th International Conference on Arabidopsis Research
  • Place of Presentation: Paris （France）
  • Year and Date: 2015-07-05
  • Invited
• [Presentation] 植物におけるTGNの動態と生理機能 (2015)
  • Author(s): 植村知博、中野明彦
  • Organizer: 第67回日本細胞生物学会大会
  • Place of Presentation: タワーホール堀船（東京都江戸川区）
  • Invited
• [Presentation] 植物におけるトランスゴルジネットワーク（ＴＧＮ）の動態と生理機能 (2015)
  • Author(s): 植村知博
  • Organizer: 第38回日本分子生物学会年会・第88回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Invited