Development of a novel fluorescent labeling method for detecting heterooligomers of membrane proteins on living cells

• Project/Area Number: 15H04636
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Kyoto University
• Principal Investigator: Katsumi Matsuzaki 京都大学, 薬学研究科, 教授 (00201773)
• Co-Investigator(Kenkyū-buntansha): 河野 健一 京都大学, 化学研究所, 助教 (70732874)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
• Keywords: 蛍光イメージング / 膜タンパク質

Outline of Final Research Achievements

New live-cell labeling methods were examined to detect GPCR hetero-oligomers in combination with the conventional coiled-coil labeling method. 1) β-hairpin and 2) RNA aptamer labeling principles were not successful because of the insufficient expression of tagged receptors on the cell surface. 3) Collagen heterodimer probe peptides and collagen-tagged GPCR were also prepared to examine the expression of the tagged receptors and selective labeling.