Multi-hierarchical analysis of the role of TRPM4 subfamily members in cardiac atrial arrhythmogenicity

• Project/Area Number: 15H04678
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: Fukuoka University
• Principal Investigator: Inoue Ryuji 福岡大学, 医学部, 教授 (30232573)
• Co-Investigator(Kenkyū-buntansha): 沼田 朋大 福岡大学, 医学部, 講師 (20455223) ; 朱 欣 会津大学, コンピュータ理工学部, 上級准教授 (70448645)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000); Fiscal Year 2018: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
• Keywords: 不整脈 / 生体ストレス / 心房リモデリング / 多階層シミュレーション / システム生理学 / TRPチャネル / 分子電気生理学 / TRPMチャネル

Outline of Final Research Achievements

Atrial fibrillation (Af) is a common arrhythmic disorder rapidly increasing in developed countries, and shows complex pathogenesis involving both structural and electrical remodeling that is frequently associated with other cardiac and metabolic/systemic diseases. This study aimed to comprehensively understand how stress-responsive molecules such as TRPM4 and TRPM7 channels can contribute to Af pathogenesis and some conduction disorders as well, by the combination of electrophysiological measurement and numerical simulation. The results, in particular, from cocultured cardiomyocyte/fibroblast monolayers and their equivalent 2D-model-based simulations suggested the critical importance of the synergy between excessive TRPM channel activity and fibroblast activation in promoting the arrhythmogenic processes.

Academic Significance and Societal Importance of the Research Achievements

近年、心房細動等の心リモデリング関連不整脈の研究領域では、心線維芽細胞の活性化やストレス応答分子TRPチャネルの発現・活性増加が新たな「基質」として注目を集めつつある。しかしこれまでは分子生物学的手法が主体であったため、その複雑な病態形成過程を統合的にとらえることは困難であった。本研究は、電気生理学実験と多階層の数理シミュレーションを融合させることで、心線維化とTRPM4チャネル過剰活性化の相乗的相互作用の重要性を定量的に示した点に意義がある。またこの研究で用いた手法は、ベンチトップレベルで遂行できることから、同分野の研究者コミュニティーに広く普及し今後の研究の進展に資することが期待される。

Research Products

• [Journal Article] SGLT2 inhibitor ipragliflozin attenuates breast cancer cell proliferation (2020)
  • Author(s): Komatsu Shiho、Nomiyama Takashi、Numata Tomohiro、Kawanami Takako、Hamaguchi Yuriko、Iwaya Chikayo、Horikawa Tsuyoshi、Fujimura-Tanaka Yuki、Hamanoue Nobuya、Motonaga Ryoko、Tanabe Makito、Inoue Ryuji、Yanase Toshihiko、Kawanami Daiji
  • Journal Title: Endocrine Journal Volume: 67 Issue: 1 Pages: 99-106
  • DOI: 10.1507/endocrj.EJ19-0428
  • NAID: 130007790952
  • ISSN: 0918-8959, 1348-4540
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Contribution of Coiled-Coil Assembly to Ca2+/Calmodulin-Dependent Inactivation of TRPC6 Channel and its Impacts on FSGS-Associated Phenotypes (2019)
  • Author(s): Polat Onur K.、Uno Masatoshi、Maruyama Terukazu、Tran Ha Nam、Imamura Kayo、Wong Chee Fah、Sakaguchi Reiko、Ariyoshi Mariko、Itsuki Kyohei、Ichikawa Jun、Morii Takashi、Shirakawa Masahiro、Inoue Ryuji、Asanuma Katsuhiko、Reiser Jochen、Tochio Hidehito、Mori Yasuo、Mori Masayuki X.
  • Journal Title: Journal of the American Society of Nephrology Volume: 30 Issue: 9 Pages: 1587-1603
  • DOI: 10.1681/asn.2018070756
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Construction of a Fluorescent Screening System of Allosteric Modulators for the GABAAReceptor Using a Turn-On Probe (2019)
  • Author(s): Sakamoto Seiji、Yamaura Kei、Numata Tomohiro、Harada Fumio、Amaike Kazuma、Inoue Ryuji、Kiyonaka Shigeki、Hamachi Itaru
  • Journal Title: ACS Central Science Volume: 5 Issue: 9 Pages: 1541-1553
  • DOI: 10.1021/acscentsci.9b00539
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] TRP channels in cardiac and intestinal fibrosis. (2019)
  • Author(s): Ryuji Inoue, Lin-hai Kurahara, Keizo Hiraishi.
  • Journal Title: Seminars in Cell and Developmental Biology. Volume: 94 Pages: 40-49
  • DOI: 10.1016/j.semcdb.2018.11.002
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Integrative and theoretical research on the architecture of a biological system and its disorder (2019)
  • Author(s): Shinichi Uchida, Yoshiyuki Asai, Yoshiaki Kariya, Kunichika Tsumoto, Hiroshi Hibino, Masashi Honma, Takeshi Abe, Fumiaki Nin, Yasutaka Kurata, Kazuharu Furutani, Hiroshi Suzuki, Hiroaki Kitano, Ryuji Inoue, Yoshihisa Kurachi
  • Journal Title: The Journal of Physiological Sciences Volume: 69 Issue: 3 Pages: 443-451
  • DOI: 10.1007/s12576-019-00667-8
  • NAID: 40021874158
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Implications of immune-inflammatory responses in smooth muscle dysfunction and disease (2019)
  • Author(s): Usui-Kawanishi Fumitake、Takahashi Masafumi、Sakai Hiroyasu、Suto Wataru、Kai Yuki、Chiba Yoshihiko、Hiraishi Keizo、Kurahara Lin Hai、Hori Masatoshi、Inoue Ryuji
  • Journal Title: Journal of Smooth Muscle Research Volume: 55 Issue: 0 Pages: 81-107
  • DOI: 10.1540/jsmr.55.81
  • NAID: 130007795217
  • ISSN: 0916-8737, 1884-8796
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Activation of Myofibroblast TRPA1 by Steroids and Pirfenidone Ameliorates Fibrosis in Experimental Crohn's Disease (2018)
  • Author(s): Kurahara Lin Hai、Hiraishi Keizo、Hu Yaopeng、Koga Kaori、Onitsuka Miki、Doi Mayumi、Aoyagi Kunihiko、Takedatsu Hidetoshi、Kojima Daibo、Fujihara Yoshitaka、Jian Yuwen、Inoue Ryuji
  • Journal Title: Cellular and Molecular Gastroenterology and Hepatology Volume: 5 Issue: 3 Pages: 299-318
  • DOI: 10.1016/j.jcmgh.2017.12.005
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Strategy to Attain Remarkably High Photoinduced Charge-Separation Yield of Donor-Acceptor Linked Molecules in Biological Environment via Modulating Their Cationic Moieties (2017)
  • Author(s): N. Cai, Y. Takano, T. Numata, R. Inoue, Y. Mori, T. Murakami, and H. Imahori
  • Journal Title: J. Phys. Chem. C Volume: 121 Issue: 32 Pages: 17457-17465
  • DOI: 10.1021/acs.jpcc.7b04466
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Integrative Approach with Electrophysiological and Theoretical Methods Reveals a New Role of S4 Positively Charged Residues in PKD2L1 Channel Voltage-Sensing (2017)
  • Author(s): Numata Tomohiro、Tsumoto Kunichika、Yamada Kazunori、Kurokawa Tatsuki、Hirose Shinichi、Nomura Hideki、Kawano Mitsuhiro、Kurachi Yoshihisa、Inoue Ryuji、Mori Yasuo
  • Journal Title: Sci. Rep. Volume: 7 Issue: 1 Pages: 9760-9760
  • DOI: 10.1038/s41598-017-10357-3
  • NAID: 120006364088
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Uncovering the arrhythmogenic potential of TRPM4 activation in atrial-derived HL-1 cells using novel recording and numerical approaches (2017)
  • Author(s): Hu Yaopeng、Duan Yubin、Takeuchi Ayako、Hai-Kurahara Lin、Ichikawa Jun、Hiraishi Keizo、Numata Tomohiro、Ohara Hiroki、Iribe Gentaro、Nakaya Michio、Mori Masayuki X.、Matsuoka Satoshi、Ma Genshan、Inoue Ryuji
  • Journal Title: Cardiovascular Research Volume: 113 Issue: 10 Pages: 1243-1255
  • DOI: 10.1093/cvr/cvx117
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mechano-sensitivity of mitochondrial function in mouse cardiac myocytes (2017)
  • Author(s): Iribe Gentaro、Kaihara Keiko、Yamaguchi Yohei、Nakaya Michio、Inoue Ryuji、Naruse Keiji
  • Journal Title: Progress in Biophysics and Molecular Biology Volume: 130 Pages: 315-322
  • DOI: 10.1016/j.pbiomolbio.2017.05.015
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cardiac myofibroblast engulfment of dead cells facilitates recovery after myocardial infarction. (2017)
  • Author(s): Michio Nakaya,Kenji Watari,Mitsuru Tajima,Takeo Nakaya,Shoichi Matsuda,Hiroki Ohara,Hiroaki Nishihara,Hiroshi Yamaguchi,Akiko Hashimoto,Mitsuho Nishida,Akiomi Nagasaka,Yuma Horii,Hiroki Ono,Gentaro Iribe,Ryuji Inoue,Makoto Tsuda,Kazuhide Inoue,Akira Tanaka,Masahiko Kuroda,Shigekazu Nagata,Hitoshi Kurose
  • Journal Title: Journal of Clinical Investigation Volume: 127 Issue: 1 Pages: 383-401
  • DOI: 10.1172/jci83822
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Distinct contributions of LRRC8A and its paralogs to the VSOR anion channel from those of the ASOR anion channel (2017)
  • Author(s): Kaori Sato-Numata, Tomohiro Numata, Ryuji Inoue, Ravshan Z. Sabirov and Yasunobu Okada.
  • Journal Title: Channels Volume: 11(2) Issue: 2 Pages: 167-172
  • DOI: 10.1080/19336950.2016.1230574
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Distinct pharmacological and molecular properties of the acid-sensitive outwardly rectifying (ASOR) anion channel from those of the volume-sensitive outwardly rectifying (VSOR) anion channel (2016)
  • Author(s): Kaori Sato-Numata, Tomohiro Numata, Ryuji Inoue, Yasunobu Okada
  • Journal Title: Pflugers Archiv - European Journal of Physiology Volume: 印刷中 Issue: 5 Pages: 795-803
  • DOI: 10.1007/s00424-015-1786-1
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Screening of Transient Receptor Potential Canonical Channel Activators Identifies Novel Neurotrophic Piperazine Compounds (2016)
  • Author(s): S. Sawamura, M. Hatano, Y. Takada, K. Hino, T. Kawamura, J. Tanikawa, H. Nakagawa, H. Hase, A. Nakao, M. Hirano, R. Rotrattanadumrong, S. Kiyonaka, M.X. Mori, M. Nishida, Y. Hu, R. Inoue, R. Nagata and Y. Mori
  • Journal Title: Mol Pharmacol Volume: 89(3) Issue: 3 Pages: 348-363
  • DOI: 10.1124/mol.115.102863
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Optical control of neuronal firing via photoinduced electron transfer in donor–acceptor conjugates (2016)
  • Author(s): Yuta Takano, Tomohiro Numata, Kazuto Fujishima, Kazuaki Miyake, Kazuya Nakao, Wesley David Grove, Ryuji Inou, Mineko Kengaku, Shigeyoshi Sakaki, Yasuo Mori, Tatsuya Murakami and Hiroshi Imahori
  • Journal Title: Chemical Science Volume: n.d. Issue: 5 Pages: 3331-3337
  • DOI: 10.1039/c5sc04135j
  • NAID: 120005841415
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Discovery of allosteric modulators for GABAA receptor by ligand-directed chemistry. (2016)
  • Author(s): Kei Yamaura, Shigeki Kiyonaka, Tomohiro Numata, Ryuji Inoue, and Itaru Hamachi
  • Journal Title: Nature Chemical Biology Volume: 12(10) Issue: 10 Pages: 822-830
  • DOI: 10.1038/nchembio.2150
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Allosteric activation of membrane-bound glutamate receptors using coordination chemistry within living cells (2016)
  • Author(s): Shigeki Kiyonaka, Ryou Kubota, Yukiko Michibata, Masayoshi Sakakura, Hideo Takahashi, Tomohiro Numata, Ryuji Inoue, Michisuke Yuzaki, Itaru Hamachi
  • Journal Title: Nat. Chem. Volume: 8 Issue: 10 Pages: 958-967
  • DOI: 10.1038/nchem.2554
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Significant contribution of TRPC6 channel-mediated Ca²⁺ influx to the pathogenesis of Crohn’s disease fibrotic stenosis (2016)
  • Author(s): Kurahara LH, Hiraishi K, Sumiyoshi M, Doi M, Hu Y, Aoyagi K, Jian Y, Inoue R
  • Journal Title: Journal of Smooth Muscle Research Volume: 52 Issue: 0 Pages: 78-92
  • DOI: 10.1540/jsmr.52.78
  • NAID: 130005279430
  • ISSN: 0916-8737, 1884-8796
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] "TRP inflammation" relationship in cardiovascular system. (2015)
  • Author(s): Numata T, Takahashi K, Inoue R
  • Journal Title: Semin Immunopathol. Volume: 印刷中 Issue: 3 Pages: 339-356
  • DOI: 10.1007/s00281-015-0536-y
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Dynamics of receptor-operated Ca2+ Currents Through TRPC Channels Controlled via the PI(4,5)P2-PLC Signaling Pathway (2015)
  • Author(s): Masayuki X Mori, Kyohei Itsuki, Hideharu Hase, Seishirou Sawamura, Tatsuki Kurokawa, Yasuo Mori, Ryuji Inoue
  • Journal Title: Frontiers in Pharmacology Volume: 6 Pages: 22-22
  • DOI: 10.3389/fphar.2015.00022
  • NAID: 120005663278
  • Peer Reviewed / Open Access
• [Journal Article] Intestinal myofibroblast TRPC6 channel may contribute to stenotic fibrosis in Crohn’s disease (2015)
  • Author(s): Lin Hai Kurahara, Miho Sumiyoshi, Kunihiko Aoyagi, Kyoko Nakajima, Midori Nakagawa, Keizo Hiraishi, Ryuji Inoue
  • Journal Title: Inflammatory Bowel Diseases Volume: 21(3) Issue: 3 Pages: 496-506
  • DOI: 10.1097/mib.0000000000000295
  • Peer Reviewed / Open Access
• [Journal Article] Ventricular fibrillation mechanisms and cardiac restitutions: An investigation by simulation study on whole-heart model (2015)
  • Author(s): Yi Zheng,Daming Wei,Xin Zhu, Wenxi Chen, Koji Fukuda, and Hiroaki Shimokawa
  • Journal Title: Computers in Biology and Medicine Volume: in press Pages: 261-268
  • DOI: 10.1016/j.compbiomed.2014.06.014
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] An updated overview on TRP channels involved in cardiovascular inflammatory/fibroproliferative diseases. (2020)
  • Author(s): Ryuji Inoue，Lin Kurahara H，Keizo Hiraishi，Yuanyuan Cui.
  • Organizer: 第97回日本生理学会大会シンポジウム
  • Int'l Joint Research / Invited
• [Presentation] Diversity of Ca2＋entry mechanisms associated with vascular functions. (2020)
  • Author(s): Ryuji Inoue
  • Organizer: 第97回日本生理学会大会教育講演
  • Int'l Joint Research / Invited
• [Presentation] TRPチャネル活性化機構の数理モデル解析 (2019)
  • Author(s): 井上隆司
  • Organizer: 産業大学医学部セミナー
  • Invited
• [Presentation] Numerical model-based studies on the activation/regulation of TRP channels. (2019)
  • Author(s): Ryuji Inoue
  • Organizer: Frontiers in Biomedical Science Seminar at the Chinese University of Hong Kong, Hong Kong, China.
  • Int'l Joint Research / Invited
• [Presentation] A multi-hierarchical study on the arrhythmogenicity of a Ca-activated cation channel TRPM4. (2019)
  • Author(s): Ryuji Inoue, Yaopeng Hu, Qin Li, Lin-Hai Kurahara, Jun Ichikawa, Tomohiro Numata, Yanghua Shen, Wenfeng Shen, Xin Zhu.
  • Organizer: FAOPS2019 Symposium
  • Int'l Joint Research / Invited
• [Presentation] An integrative approach to investigating the arrhythmic TRPM4 channelopathy. (2019)
  • Author(s): Ryuji Inoue
  • Organizer: International symposium “Logic of life: ion channel structure, function and physiology” at Oska University
  • Int'l Joint Research / Invited
• [Presentation] An integrative approach to investigating remodeling-associated arrhythmias: Computer simulation based on the gating analysis of TRPM4 channel. (2018)
  • Author(s): Ryuji Inoue, Yaopeng Hu, Lin-Hai Kurahara, Jun Ichikawa, Tomohiro Numata, Yanghua Sheng, Wenfeng Sheng, Xin Zhu.
  • Organizer: 16th Nanjing Course on Cardiac Revascularization & ACS Symposium.
  • Int'l Joint Research / Invited
• [Presentation] A theoretical approach to investigating the arrhythmogenicity of TRPM4 channel. (2018)
  • Author(s): Ryuji Inoue, Yaopeng Hu, Lin-Hai Kurahara, Jun Ichikawa, Tomohiro Numata, Yanghua Sheng, Xin Zhu.
  • Organizer: NCCR TransCure Lecture at University of Bern
  • Int'l Joint Research / Invited
• [Presentation] TRPA1 channel agonists protect against intestinal inflammation and fibrosis. (2018)
  • Author(s): Kurahara Hai Lin, Hiraishi Keizo, Hu Yaopeng, Doi Mayumi, Koga Kaori, Onitsuka Miki, Inoue Ryuji.
  • Organizer: Symposium 8. The 95th Annual Meeting of the Physiological Society of Japan. 28 March 2018, Takamatsu Tower Hall, Japan.
• [Presentation] Integrative approach with electrophysiological and theoretical methods reveals an unusual voltage sensing mechanism in PKD2L1 channel. (2018)
  • Author(s): Numata Tomohiro, Inoue Ryuji.
  • Organizer: Symposium 21. The 95th Annual Meeting of the Physiological Society of Japan. 30 March 2018. Takamatsu Tower Hall, Japan.
• [Presentation] Multi-hierarchical analysis of TRPM4 arrhythmogenicity by experimental and numerical approaches. (2018)
  • Author(s): Hu Yaopeng, Shen Yanghua, Kurahara Lin, Hiraishi Keizo, Ichikawa Jun, Numata Tomohiro, Okamura Yasushi, Zhu Xin, Inoue Ryuji.
  • Organizer: Symposium 21. The 95th Annual Meeting of the Physiological Society of Japan. 30 March 2018. Takamatsu Tower Hall, Japan.
• [Presentation] Microarray analysis of electrically-modulated molecules that regulate bone marrow stromal cell (BMSC) proliferation. (2018)
  • Author(s): Ichikawa Jun, Inoue Ryuji.
  • Organizer: 1P-063. The 95th Annual Meeting of the Physiological Society of Japan. 28 March 2018, Takamatsu Tower Hall, Japan.
• [Presentation] Properties and roles of flufenamate-sensitive ion channels stimulated by hyperosmolality in vasopressin neurons. (2018)
  • Author(s): Sato-Numata Kaori, Numata Tomohiro, Ueta Yoichi, Inoue Ryuji, Okada Yasunobu
  • Organizer: 3P-068. The 95th Annual Meeting of the Physiological Society of Japan. 30 March 2018, Takamatsu Tower Hall, Japan.
• [Presentation] Contribution of tyrosine kinase FYN to vascular remodeling in pulmonary arterial hypertension. (2018)
  • Author(s): Hiraishi Keizo, Kurahara Lin, Zhang Ying, Yamamura Aya, Kishi Hiroko, Kobayashi Sei, Koga Kaori, Onitsuka Miki, Inoue Ryuji.
  • Organizer: 3P-160. 30 March 2018, Takamatsu Tower Hall, Japan.
• [Presentation] Electrophysiological and simulation analyses of atrial excitation/propagation in a co-cultured HL-1 cardiomyocytes-cardiac fibroblast monolayer. (2017)
  • Author(s): Yaopeng Hu, Yanghua Shen, Keizo Hiraishi, Lin Kurahara, Jun Ichikawa, Tomohiro Numata, Wenfeng Shen, Xin Zhu, Ryuji Inoue.
  • Organizer: The 94th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: ACT CITY, Hamamatsu, Japan
  • Year and Date: 2017-03-28
• [Presentation] Pathophysiological contribution of endothelial TRPM7 channel to pulmonary arterial hypertension. (2017)
  • Author(s): Keizo Hiraishi, Kin Kurahara, Yaopeng Hu, Kaori Koga, Miki Onitsuka, Kotaro Abe, Toru Sato, Yasumasa Okada, Ryuji Inoue.
  • Organizer: The 94th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: ACT CITY Hamamatsu, Japan
  • Year and Date: 2017-03-28
• [Presentation] Possible involvement of TRPM7-mediated cation influx in K562 heamtopoietic cell growth and differentiation. (2017)
  • Author(s): Kiriko Takahashi, Tomohiro Numata, Ken Yamamura, Ryuji Inoue.
  • Organizer: The 94th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: ACT CITY Hamamatsu, Japan
  • Year and Date: 2017-03-28
• [Presentation] Protective effects of the Transient Receptor Potential Ankyrin 1 Ion Channel in the colon against inflammation and fibrosis. (2017)
  • Author(s): Lin Kurahara, Keizo Hiraishi, Yaopeng Hu, Kaori Koga, Miki Onitsuka, Ryuji Inoue.
  • Organizer: The 94th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: ACT CITY Hamamatsu, Japan
  • Year and Date: 2017-03-28
• [Presentation] Electrical stimulations do not affect the expression patterns of TRPC6 and other effectors regulating bone marrow stromal cell (BMSC) proliferation. (2017)
  • Author(s): Jun Ichikawa, Ryuji Inoue
  • Organizer: The 94th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: ACT CITY Hamamatsu, Japan
  • Year and Date: 2017-03-28
• [Presentation] TRPチャネル活性化機構の数理モデル解析 (2017)
  • Author(s): 井上隆司
  • Organizer: 生理学研究所 研究会2017 「心臓・血管系の頑健性と精緻な制御を支える分子基盤の統合的解明」
  • Invited
• [Presentation] Contribution of TRPM7 and FYN to Endothelial remodeling in HUVECs and HPAECs. (2016)
  • Author(s): Keizo Hiraishi, Lin-Hai Kurahara, and Ryuji Inoue
  • Organizer: 2016 International Conference of Physiological Sciences
  • Place of Presentation: Beijing, China
  • Year and Date: 2016-09-25
  • Int'l Joint Research
• [Presentation] Abnormal automaticity of HL-1 cardiomyocyte induced via Ca- and voltage-dependent activation of TRPM4 channels. (2016)
  • Author(s): Ryuji Inoue, Hu Yaopeng, Xin Zhu, Tomohiro Numata
  • Organizer: The 13th Japan-Korea Joint Symposium on Brain Science, and Cardiac and Smooth Muscles.
  • Place of Presentation: Saga University, Saga, Japan
  • Year and Date: 2016-08-25
  • Int'l Joint Research / Invited
• [Presentation] An arrhythmic mutation modifies TRPM4 channel gating via altered PIP2 sensitivity (2016)
  • Author(s): Hu Y, Kurahara LH, Shioi N, Hiraishi K, Ichikawa J, Numata T, Okamura Y, Inoue R
  • Organizer: Symposium 20 “PIPs-protein interaction in cell physiology”. The 93rd annual Meeting of Physiological Society of Japan
  • Place of Presentation: Sapporo, Sapporo Convention Center
  • Year and Date: 2016-03-24
  • Invited
• [Presentation] Numerical model-based investigation of TRPM4 channel in cardiac remodeling-associated arrhythmogenicity (2016)
  • Author(s): Inoue R, Hu Y, Duan Y, Takeuchi A, Kurahara LH, Ichikawa J, Matsuoka S
  • Organizer: Symposium 12 “High Definition Physiology in the Heart, from Molecule to Organ”. The 93rd annual Meeting of Physiological Society of Japan
  • Place of Presentation: Sapporo, Sapporo Convention Center
  • Year and Date: 2016-03-23
  • Invited
• [Presentation] Simulation-based study of PIP2-meditated regulation of TRP channels based on voltage-sensing phosphatase and FRET measurements (2015)
  • Author(s): Inoue R, Hu Y, Ichikawa J, Kurahara LH, Numata T
  • Organizer: Symposium VIII “The roles of TRP channels in cardiovascular system”, The 67th Annual Meeting of Korean Physiological Society
  • Place of Presentation: Moam Hall, Pusan National University Hospital, Busan, Korea
  • Year and Date: 2015-10-23
  • Int'l Joint Research / Invited
• [Presentation] Dynamic regulation of TRPC and TRPM4 channels by endogenous PIP2 level investigated by voltage-sensing phosphatase and FRET (2015)
  • Author(s): Inoue R
  • Organizer: Keynote lecture, the 50th Annual Congress of the Brazilian Society of Physiology (SBFis 2015)
  • Place of Presentation: Aquas Lindoia, San Paulo, Brazil
  • Year and Date: 2015-08-05
• [Presentation] A FRET- and simulation-based study on the PIP2-mediated regulation of TRPC3/C6/C7 subfamily channels (2015)
  • Author(s): Inoue R, Itsuki K, Imai U, Okamura Y, Mori MX
  • Organizer: Symposium “Multicellular Inputs Regulating Muscle Excitability”
  • Place of Presentation: Granlibakken, Lake Tahoe, California, USA
  • Year and Date: 2015-06-14
  • Int'l Joint Research / Invited
• [Book] TRPM4チャネルと心血管の生理・病態生理：医学のあゆみ_270巻10号、特集「TRPチャネルのすべて」 (2019)
  • Author(s): 井上隆司、 胡耀鵬、沼田朋大
  • Total Pages: 6
  • Publisher: 医歯薬出版
• [Book] TRPCサブファミリーの分子構造と活性化・制御機序の新知見： 医学のあゆみ_270巻10号、特集「TRPチャネルのすべて」 (2019)
  • Author(s): 井上隆司、市川純、崔媛媛
  • Total Pages: 7
  • Publisher: 医歯薬出版
• [Book] Intestinal Fibroblast/Myofibroblast TRP Channels in Inflammatory Bowel Disease. In; "New Insights into Inflammatory Bowel Disease", Chapter 8 (2016)
  • Author(s): Lin Hai Kurahara, Keizo Hiraishi, Kunihiko Aoyagi, Yaopeng Hu, Miho Sumiyoshi, Ryuji Inoue
  • Total Pages: 28
  • Publisher: InTechOpen; ISBN 978-953-51-2754-3
• [Remarks] 福岡大学医学部生理学講座ホームページ/研究紹介
  • URL: http://www.med.fukuoka-u.ac.jp/physiol/study1.html
• [Remarks] 個人ホームページ（福岡大学医学部生理学教室：井上隆司）
  • URL: http://www.med.fukuoka-u.ac.jp/physiol/index-j.htm
• [Remarks] CardiacSimu（v.0.0.3.1）
  • URL: http://cardiacsimu.sourceforge.net/index.htm