| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Outline of Final Research Achievements |
Aquaporin4 (AQP4) has been shown to be involved in the development of brain edema. We recently developed a high throughput-screening system for AQP4, as a target molecule for the treatment of brain edema. In this project, we identified at least one chemical compound that inhibits AQP4, by using a stopped flow apparatus with AQP4 proteoliposome. Computer based docking study revealed that this chemical may interact with the extracelluar domain of AQP4. Next, we examined molecular mechanisms of AQP4 endocytosis to understand the molecular regulation of AQP4. We have identified three signals for; i) trafficking to the plasma membranes, ii) protein stability and iii) sorting to lysosomes.
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