| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2015: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
Dendritic cells (DCs) are consisted conventional DCs and plasmacytoid DC (pDCs) which have antigen presentation capacity and type-I interferon producing capacity, respectively. In this study, we generate E2-2 reporter mouse which is essential transcription factor for pDC development. Using this mouse, we identified E2-2high and E2-2lo cells in th common DC progenitor (CDPs). E2-2highCDPs develop only pDC in vivo in the spleen, however, in the small intestine, some E2-2highCDPs develop into cDC. This trans-differentiation is mediated by IL-3, IL-5, GM-CSF (all enriched in the gut tissue). In ex vivo cultures with Flt3L+GM-CSF, E2-2highCDPs derived cDCs, but not pDCs, induce Foxp3+ Treg. E2-2 is therefore important in committing pDC differentiation but conversion to cDCs can occur in the intestine and modulate regulatory environment there.
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