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Mechanism for the formation of cheating organella of Toxoplasma gondii

Research Project

Project/Area Number 15H04726
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Parasitology (including sanitary zoology)
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

Nagamune Kisaburo  国立感染症研究所, 寄生動物部, 室長 (90314418)

Co-Investigator(Kenkyū-buntansha) 案浦 健  国立感染症研究所, 寄生動物部, 主任研究官 (90407239)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2015: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywords原虫 / オルガネラ / リクルート / トキソプラズマ
Outline of Final Research Achievements

It is known that Toxoplasma gondii can recruit the host mitochondria to its parasitophorous vacuole. First of this study, I identified 7 candidates responsible for this phenomenon, and evaluate the ability for the recruitment using with overexpressors. As a result, I could identify four genes responsible for the mitochondrial recruitment of T. gondii. I further tried to knock-out these genes. However, I could not knock-out three of them because they might be essential. I established knock-out mutant of remaining gene. This mutant decreased the recruitment ability of the host mitochondria.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (33 results)

All 2018 2017 2016 2015

All Journal Article (13 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 12 results,  Open Access: 11 results,  Acknowledgement Compliant: 2 results) Presentation (15 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Book (5 results)

  • [Journal Article] Toxoplasma gondii seroprevalence in goats, cats and humans in Russia2018

    • Author(s)
      Shuralev, E.A., Shamaev, N.D., Mukminov, M.N., Nagamune, K., Taniguchi, Y., Saito, T., Kitoh, K., Arleevskaya, M., Fedotova, A.Y., Abdulmanova, D.R., Aleksandrova, N.M., Efimova, M.A., Yarullin, A.I., Valeeva, A.R., Khaertynov, K.S., Takashima, Y.
    • Journal Title

      Parasitol. Int.

      Volume: 67 Issue: 2 Pages: 112-114

    • DOI

      10.1016/j.parint.2017.10.014

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Toxoplasma gondii RON4 binds to heparan sulfate on the host cell surface2018

    • Author(s)
      Takemae, H., Kobayashi, K., Sugi, T., Han, Y., Gong, H., Ishiwa, A., Recuenco, F. C., Murakoshi, F., Takano, R., Murata, Y., Nagamune, K., Horimoto, T., Akashi, H., Kato, K.
    • Journal Title

      Parasitol. Int.

      Volume: 67 Issue: 2 Pages: 123-130

    • DOI

      10.1016/j.parint.2017.10.008

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Fates of Evolutionarily Distinct, Plastid- type Glyceraldehyde 3-phosphate Dehydrogenase Genes in Kareniacean Dinoflagellates2018

    • Author(s)
      Kamikawa, R., Yazaki, E., Tahara, M., Sakura, T., Matsuo, E., Nagamune, K., Hashimoto, T., Inagaki, Y.
    • Journal Title

      J. Eukaryot. Microbiol.

      Volume: 印刷中 Issue: 5 Pages: 669-678

    • DOI

      10.1111/jeu.12512

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Plant hormone cytokinins control cell cycle progression and plastid replication in apicomplexan parasites2017

    • Author(s)
      Andrab, S.B.A., Tahara,M., Matsubara,R., Toyama,T., Aonuma,H., Sakakibara,H., Suematsu,M., Tanabe,K., Nozaki,T., Nagamune, K.
    • Journal Title

      Parasitol. Int.

      Volume: 67 Issue: 1 Pages: 47-58

    • DOI

      10.1016/j.parint.2017.03.003

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] トキソプラズマが宿主細胞のオルガネラを引き寄せるメカニズム2017

    • Author(s)
      福本隼平、永宗喜三郎
    • Journal Title

      医学と薬学

      Volume: 74 Pages: 1217-1220

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Journal Article] In vitro and in vivo effects of the phytohormone inhibitor fluridone against Neospora caninum infection2016

    • Author(s)
      bañez, R.H., Leesombun, A., Nishimura, M., Matsubara, R., Kojima, M., Sakakibara, H., Nagamune, K., Nishikawa, Y.
    • Journal Title

      Parasitol. Int.

      Volume: 65 Issue: 4 Pages: 319-322

    • DOI

      10.1016/j.parint.2016.03.009

    • NAID

      120006390951

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A host cell membrane microdomain is a critical factor for organelle discharge by Toxoplasma gondii2016

    • Author(s)
      Tahara, M., Andrabi, S.B., Matsubara, R., Aonuma, H., Nagamune K.
    • Journal Title

      Parasitol. Int.

      Volume: 65 Issue: 5 Pages: 378-388

    • DOI

      10.1016/j.parint.2016.05.012

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Toxoplasma gondii Infection in Mice Impairs Long-Term Fear Memory Consolidation Through Dysfunction of the Cortex and Amygdala2016

    • Author(s)
      Ihara, F., Nishimura, M., Muroi, Y., Mahmoud, M.E., Yokoyama, N., Nagamune, K., Nishikawa, Y.
    • Journal Title

      Infect Immun.

      Volume: 84 Issue: 10 Pages: 2861-2871

    • DOI

      10.1128/iai.00217-16

    • NAID

      120006390959

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Investigation into the Physiological Significance of the Phytohormone Abscisic Acid in Perkinsus marinus, an Oyster Parasite Harboring a Non-Photosynthetic Plastid2016

    • Author(s)
      Sakamoto, H, Suzuki, S., Nagamune, K., Kita, K., Matsuzaki, M.
    • Journal Title

      J. Eukaryot. Microbiol.

      Volume: 印刷中 Issue: 4 Pages: 440-446

    • DOI

      10.1111/jeu.12379

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Increased CD11b+ Gr-1+ cell population in the placenta after infection with Toxoplasma gondii.2015

    • Author(s)
      Takeshima K, Sato K, Nabekura T, Nagamune K, Hamada H, Yoshikawa H, Shibuya A, Shibuya K.
    • Journal Title

      Microbiol. immunol

      Volume: 59 Issue: 2 Pages: 95-98

    • DOI

      10.1111/1348-0421.12225

    • NAID

      120007130301

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Prevalence and dynamics of antibodies against Toxoplasma gondii in kids born from naturally infected goats2015

    • Author(s)
      Rahman, M., Alauddin, M., Hossain, K.M., Islam, M.H., Kitoh, K., Nagamune, K., Takashima, Y.
    • Journal Title

      Parasitol. Int.

      Volume: 64 Issue: 5 Pages: 389-391

    • DOI

      10.1016/j.parint.2015.05.015

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evaluation of aegerolysins as novel tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates2015

    • Author(s)
      Bhat HB, Ishitsuka R, Inaba T, Murate M, Abe M, Makino A, Kohyama-Koganeya A, Nagao K, Kurahashi A, Kishimoto T, Tahara M, Yamano A, Nagamune K, Hirabayashi Y, Juni N, Umeda M, Fujimori F, Nishibori K, Yamaji-Hasegawa A, Greimel P, Kobayashi T.
    • Journal Title

      FASEB J

      Volume: 29(9) Issue: 9 Pages: 3920-34

    • DOI

      10.1096/fj.15-272112

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Plant Hormone Salicylic Acid Produced by a Malaria Parasite Controls Host Immunity and Cerebral Malaria Outcome.2015

    • Author(s)
      Matsubara R, Aonuma H, Kojima M, Tahara M, Andrabi SB, Sakakibara H, Nagamune K.
    • Journal Title

      PLoS One

      Volume: 10 Issue: 10 Pages: e0140559-e0140559

    • DOI

      10.1371/journal.pone.0140559

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] トキソプラズマにおける宿主ミトコンドリアリクルート機構の解明2018

    • Author(s)
      福本隼平、佐倉孝哉、松原立真、田原美智留、松崎素道、永宗喜三郎
    • Organizer
      第87 回日本寄生虫学会大会
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Presentation] トキソプラズマ日本分離株のゲノムワイドSNP解析2018

    • Author(s)
      松崎素道、福本隼平、喜屋武向子、正谷達謄、松尾智英、村上麻美、高島康弘、西川義文、永宗喜三郎
    • Organizer
      第87 回日本寄生虫学会大会
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Presentation] 肝内型マラリア原虫における核増殖メカニズムの解明2018

    • Author(s)
      荒木球沙、川合覚、小林宏尚、片岡紀代、角田宗一郎、永宗喜三郎、野崎智義、久枝一、案浦健
    • Organizer
      第87 回日本寄生虫学会大会
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Presentation] ナショナルデータベース(NDB Japan)を用いた日本国内の寄生虫症発生動向の解析2018

    • Author(s)
      森嶋康之、永宗喜三郎、杉山 広、山﨑 浩
    • Organizer
      第87 回日本寄生虫学会大会
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Presentation] レセプト情報解析から見えてきた日本におけるトキソプラズマ症の実態2018

    • Author(s)
      永宗喜三郎、森嶋康之
    • Organizer
      第87 回日本寄生虫学会大会
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Presentation] 日本におけるトキソプラズマのタイピングと病原性2017

    • Author(s)
      福本隼平、山野安規徳、松原立真、竹内史比古、喜屋武向子、正谷達謄、松尾智英、村上麻美、高島康弘、永宗喜三郎
    • Organizer
      第86 回日本寄生虫学会大会
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Presentation] 熱帯熱マラリア原虫の赤内期における新しい輸送経路の解析2017

    • Author(s)
      北園和泉、平井智浩、永宗喜三郎、野崎智義、中野由美子
    • Organizer
      第25 回分子寄生虫学ワークショップ・第14回分子寄生虫・マラリア研究フォーラム合同大会
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Presentation] 肝内型マラリア原虫における核増殖メカニズムの解明2017

    • Author(s)
      荒木球沙、川合覚、小林宏尚、片岡紀代、永宗喜三郎、野崎智義、案浦健
    • Organizer
      第50回日本原生生物学会、第1回日本共生生物学会合同大会
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Presentation] トキソプラズマにおける宿主ミトコンドリアリクルート機構の解明2017

    • Author(s)
      福本隼平、佐倉孝哉、松原立真、田原美智留、永宗喜三郎
    • Organizer
      第50回日本原生生物学会、第1回日本共生生物学会合同大会
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Invited
  • [Presentation] The molecular genotyping and dissection of Japanese Toxoplasma gondii reveals an unidentified strategy for successful survival of the parasite2017

    • Author(s)
      Nagamune, K., Fukumoto, J., Yamano, A., Tahara, M., Matsubara, R.
    • Organizer
      14th The Toxoplasma gondii research community biennial meeting
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Elucidating the mechanism of host mitochondrial recruitment of Toxoplasma gondii2016

    • Author(s)
      Fukumoto, J., Sakura, T., Matsubara, R., Nagamune, K.
    • Organizer
      The 13th International Colloquium on Endocytobiology and Symbiosis
    • Place of Presentation
      京都市
    • Year and Date
      2016-09-10
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] トキソプラズマにおける宿主オルガネラリクルート機構の解析にむけて2016

    • Author(s)
      福本隼平、佐倉孝哉、永宗喜三郎
    • Organizer
      第85回日本寄生虫学会大会
    • Place of Presentation
      宮崎市
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] トキソプラズマにおける宿主オルガネラリクルート機構の解析にむけて2015

    • Author(s)
      福本隼平、佐倉孝哉、松原立真、田原美智留、永宗喜三郎
    • Organizer
      第48回日本原生生物学会大会
    • Place of Presentation
      東京都
    • Year and Date
      2015-11-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] Investigation for the Understanding of the Mechanism of Host Organelle Recruitment by Toxoplasma gondii2015

    • Author(s)
      Fukumoto, J., Sakura, T., Matsubara, R., Nagamune, K.
    • Organizer
      2nd International Symposium and 4th Annual Research Meeting on Matryoshka-type Evolution of Eukaryotic Cells
    • Place of Presentation
      つくば市
    • Year and Date
      2015-09-30
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] トキソプラズマにおける宿主オルガネラリクルート機構の解析にむけて2015

    • Author(s)
      福本隼平、佐倉孝哉、松原立真、田原美智留、永宗喜三郎
    • Organizer
      第23 回分子寄生虫学ワークショップ・第13回分子寄生虫・マラリア研究フォーラム合同大会
    • Place of Presentation
      帯広市
    • Year and Date
      2015-08-30
    • Related Report
      2015 Annual Research Report
  • [Book] 小児臨床検査のポイント20172017

    • Author(s)
      永宗喜三郎
    • Total Pages
      4
    • Publisher
      東京医学社
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Book] 小児内科2017

    • Author(s)
      永宗喜三郎
    • Publisher
      東京医学社
    • Related Report
      2015 Annual Research Report
  • [Book] 化学療法の領域2016

    • Author(s)
      松原立真、永宗喜三郎
    • Total Pages
      10
    • Publisher
      医薬ジャーナル社
    • Related Report
      2015 Annual Research Report
  • [Book] 遺伝2016

    • Author(s)
      松原立真、永宗喜三郎
    • Total Pages
      6
    • Publisher
      エヌ・ティー・エス
    • Related Report
      2015 Annual Research Report
  • [Book] 衛生の友2016

    • Author(s)
      永宗喜三郎
    • Total Pages
      1
    • Publisher
      サラヤ
    • Related Report
      2015 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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