| Project/Area Number |
15H04923
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Kyoto University (2016-2017)
National Cardiovascular Center Research Institute (2015) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
山岡 哲二 国立研究開発法人国立循環器病研究センター, 研究所, 部長 (50243126)
大高 晋之 国立研究開発法人国立循環器病研究センター, 研究所, 流動研究員 (30739561)
MUNISSO Maria Chiara 国立研究開発法人国立循環器病研究センター, 研究所, 特任研究員 (50616084)
馬原 淳 国立研究開発法人国立循環器病研究センター, 研究所, 研究員 (80416221)
徐 ユイ 国立研究開発法人国立循環器病研究センター, 研究所, 流動研究員 (10757678)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2016: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
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| Keywords | 人工臓器学 / バイオテクノロジー / 循環器・高血圧 |
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| Outline of Final Research Achievements |
A conjugate molecule of a etiologic molecule-binding molecule and an ApoE molecule that binds to the liver LDL receptor was synthesized as a "navigator" molecule, which is a novel drug that navigate the target etiologic molecule to the liver and reduce it blood level. First, it was confirmed in vitro that the navigator molecule binds well with the target molecule, and hepatocyte selectively uptake the navigating. When cultured at 37 degree C the fluorescence intensity in the hepatocytes became much lower than when cultured at 4 degree C, which suggests that the navigator was taken up by the hepatocytes and degraded in the cells. Furthermore, we investigated the body distribution of the navigator molecule in the mouse, and found that the liver accumulated was significantly higher, indicating the basic POC.
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