Lipid and steroid metabolism and prostate cancer using imaging mass-spectrometry

• Project/Area Number: 15H04971
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Gunma University
• Principal Investigator: Suzuki Kazuhiro 群馬大学, 大学院医学系研究科, 教授 (80312891)
• Co-Investigator(Kenkyū-buntansha): 伊藤 一人 群馬大学, 大学院医学系研究科, 准教授 (00302472) ; 関根 芳岳 群馬大学, 大学院医学系研究科, 助教 (00516370) ; 松井 博 群馬大学, 重粒子線医学推進機構, 講師 (40450374) ; 柴田 康博 群馬大学, 医学部附属病院, 講師 (90344936) ; 小池 秀和 群馬大学, 医学部附属病院, 講師 (90420091)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000); Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2016: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 前立腺癌 / 脂質 / ステロイド / イメージング質量分析 / 脂質代謝 / ステロイド代謝 / 質量分析

Outline of Final Research Achievements

Prostate cancer is an adrogen-dependent cancer. Lipid, which is a substrate of androgen, plays an important role on prostate cancer proliferation. In this study, we studied the localization and mass-distribution of testosterone, dihydrotestosterone and cholesterol in prostate cancer tissues using the imaging mass-spectrometry. Testosterone, dihydrotestosterone and cholesterol have their own molecular wights, and these molecules were able to be quantified by mass-spectrometry. Simultaneous histological analyses were feasible, and imaging mass-spectromery would be considered as efficient. We also studied the role of statin, inhibitor of HMG-CoA reductase, on prostate cancer proliferation. We found that LDL receptor and annexin A10 were involved in the regulation of prostate cancer proliferation under control of statin.

Research Products

• [Journal Article] Simvastatin in combination with meclofenamic acid inhibits the proliferation and migration of human prostate cancer PC-3 cells via an AKR1C3 mechanism. (2018)
  • Author(s): Sekine Y, Nakayama H, Miyazawa Y, Kato H, Furuya Y, Arai S, Koike H, Matsui H, Shibata Y, Ito K, Suzuki K.
  • Journal Title: Oncology Letters Volume: 15 Pages: 3167-3172
  • DOI: 10.3892/ol.2017.7721
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effects of Steroidal Antiandrogen or 5-alpha-reductase Inhibitor on Prostate Tissue Hormone Content. (2017)
  • Author(s): Shibata Y, Arai S, Miyazawa Y, Shuto T, Nomura M, Sekine Y, Koike H, Matsui H, Ito K, Suzuki K.
  • Journal Title: The Prostate Volume: 72 Issue: 6 Pages: 672-688
  • DOI: 10.1002/pros.23315
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Simvastatin Up-Regulates Annexin A10 That Can Inhibit the Proliferation, Migration, and Invasion in Androgen-Independent Human Prostate Cancer Cells. (2017)
  • Author(s): Miyazawa Y, Sekine Y, Kato H, Furuya Y, Koike H, Suzuki KSimvastatin Up-Regulates Annexin A10 That Can Inhibit the Proliferation, Migration, and Invasion in Androgen-Independent Human Prostate Cancer Cells.
  • Journal Title: The Prostate Volume: 77 Issue: 4 Pages: 337-349
  • DOI: 10.1002/pros.23273
  • Peer Reviewed
• [Journal Article] Low-density lipoprotein receptors play an important role in the inhibition of prostate cancer cell proliferation by statins. (2016)
  • Author(s): Furuya Y, Sekine Y, Kato H, Miyazawa Y, Koike H, Suzuki K.
  • Journal Title: doi: 10.1016/j.prnil.2016.02.003. Volume: 4 Issue: 2 Pages: 56-60
  • DOI: 10.1016/j.prnil.2016.02.003
  • Peer Reviewed