| Project/Area Number |
15H05022
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
Shiba Hideki 広島大学, 医歯薬保健学研究科(歯), 教授 (60260668)
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| Co-Investigator(Kenkyū-buntansha) |
鈴木 茂樹 広島大学, 病院(歯), 講師 (30549762)
小武家 誠司 広島大学, 医歯薬保健学研究科(歯), 助教 (50744794)
永安 慎太郎 広島大学, 医歯薬保健学研究科(歯), 助教 (60635192)
本山 直世 広島大学, 医歯薬保健学研究科(歯), 助教 (70509661)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2017: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
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| Keywords | 歯内療法 / 再生3要素 / 細胞機能調節因子 / 宿主細胞傷害性 / LL37 / ヘパリン-LL37複合体 / 抗菌活性 / 抗炎症作用 / 歯内療法学 / 抗菌ペプチド / 歯内疾患再生治療剤 / 象牙基質タンパク質 / 骨髄間葉系幹細胞 / 間葉系幹細胞 / 細胞外基質 / 歯内治療学 |
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| Outline of Final Research Achievements |
Cells, scaffolds and regulatory factors are the three key elements for tissue engineering. LL37 possesses broad-ranged bactericidal effects and angiogenesis ability, indicating LL37 is a candidate for a regulatory factor. However, LL37 is known to exhibit cytotoxic properties for host cells. Therefore, the development of LL37-complexes, by which the cytotoxicity for host cells is eliminated without antimicrobial abilities diminished, is essential for future clinical application. We demonstrated that LL37 (10 microM) premixed with heparin (2-8 microg/ml), one of glycosaminoglycans, did not show any cytotoxic effects but retained bactericidal effects. In addition, heparin-LL37 complex had cell-functioning ability. These findings suggest that the heparin-LL37 complex is a promising regulatory factor for regenerative therapy.
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