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The development of periodontal regenerative cell therapy by using clumps of MSCs/ECM complexes

Research Project

Project/Area Number 15H05053
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Periodontology
Research InstitutionHiroshima University

Principal Investigator

Hidemi Kuriha  広島大学, 医歯薬保健学研究科(歯), 教授 (40161765)

Co-Investigator(Kenkyū-buntansha) 加治屋 幹人  広島大学, 医歯薬保健学研究科(歯), 助教 (00633041)
岩田 倫幸  広島大学, 病院(歯), 助教 (30418793)
加藤 功一  広島大学, 医歯薬保健学研究科(歯), 教授 (50283875)
水野 智仁  広島大学, 病院(歯), 講師 (60325181)
藤田 剛  広島大学, 医歯薬保健学研究科(歯), 准教授 (80379883)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Keywords間葉系幹細胞集塊 / C-MSCs / 歯周組織再生 / 立体組織 / 免疫制御能 / 凍結保存 / 間葉系幹細胞 / 細胞集塊 / C-MSC / 立体化 / 3Dバイオプリンター / 立体組織化 / 3Dプリンター / 3Dプリンター
Outline of Final Research Achievements

Previously, we have generated clumps of mesenchymal stem cells (MSCs)/extracellular matrix (ECM) complexes (C-MSCs), which consisted of cells and self-produced ECM. C-MSCs can be regulated its cellular function in vitro and be grafted into bony lesion without any artificial scaffold to induce bone regeneration.The aim of this present study is to develop stringent clinical-scale production platform for promising periodontal tissue regenerative cell therapy by using C-MSCs.
In this study, we have demonstrated that C-MSCs transplantation induced successful periodontal tissue regeneration in beagle dog class III furcation defect model. Moreover, C-MSCs can be assembled when they are cultured in contact with each other. In addition, C-MSCs treated with IFN-gamma abrogated xeno-immune response. Cryopreserved C-MSCs retain theri cellular property. These findings suggested that assembled C-MSCs generated from donor cells can be cell preparation for periodontal tissue regenerative therapy.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (17 results)

All 2018 2017 2016 2015

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (12 results) (of which Int'l Joint Research: 2 results,  Invited: 4 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Cryopreserved clumps of mesenchymal stem cell/extracellular matrix complexes retain osteogenic capacity and induce bone regeneration2018

    • Author(s)
      Motoike Souta、Kajiya Mikihito、Komatsu Nao、Takewaki Manabu、Horikoshi Susumu、Matsuda Shinji、Ouhara Kazuhisa、Iwata Tomoyuki、Takeda Katsuhiro、Fujita Tsuyoshi、Kurihara Hidemi
    • Journal Title

      Stem Cell Research & Therapy

      Volume: 9 Issue: 1 Pages: 73-73

    • DOI

      10.1186/s13287-018-0826-0

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Xenotransplantation of interferon-gamma-pretreated clumps of a human mesenchymal stem cell/extracellular matrix complex induces mouse calvarial bone regeneration2017

    • Author(s)
      Kei Takeshita, Souta Motoike, Mikihito Kajiya, Nao Komatsu, Manabu Takewaki, Kazuhisa Ouhara, Tomoyuki Iwata, Katsuhiro Takeda, Noriyoshi Mizuno, Tsuyoshi Fujita, and Hidemi Kurihara
    • Journal Title

      Stem Cell Research & Therapy

      Volume: 印刷中

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] MSC/ECM cellular complexes induce periodontal tissue regeneration2017

    • Author(s)
      Manabu Takewaki, Mikihito Kajiya*, Katsuhiro Takeda, Shinya Sasaki, Souta Motoike, Nao Komatsu, Shinji Matsuda, Kazuhisa Ouhara, Noriyoshi Mizuno, Tsuyoshi Fujita, and Hidemi Kurihara
    • Journal Title

      Journal of Dental Research

      Volume: 印刷中

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Clumps of a mesenchymal stromal cell/extracellular matrix complex can be a novel tissue engineering therapy for bone regeneration.2015

    • Author(s)
      Kittaka M, Kajiya M, Shiba H, Takewaki M, Takeshita K, Khung R, Fujita T, Iwata T, Nguyen TQ, Ouhara K, Takeda K, Fujita T, Kurihara H.
    • Journal Title

      Cytotherapy.

      Volume: 2 Issue: 7 Pages: 48-61

    • DOI

      10.1016/j.jcyt.2015.01.007

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 3次元培養間葉系幹細胞集塊の細胞分化を制御するメカノトランスダクション機構の解析2017

    • Author(s)
      加治屋幹人、小松 奈央,本池 総太, 竹脇 学, 竹下 慶, 應原 一久, 武田 克浩, 岩田 倫幸, 藤田 剛, 栗原 英見
    • Organizer
      第16回日本再生医療学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 骨髄間葉系幹細胞集塊C-MSCによって拓く未来の歯周病細胞治療法2017

    • Author(s)
      加治屋幹人
    • Organizer
      第60回日本春季歯周病学会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Clumps of a mesenchymal stem cell/extracellular matrix complex induces periodontal tissue regeneration2017

    • Author(s)
      竹脇学、加治屋幹人、武田克浩、佐々木慎也、本池総太、小松奈央、松田真司、應原一久、水野智仁、藤田 剛、栗原英見
    • Organizer
      第12回アジア太平洋歯周病学会
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 3次元培養される骨髄間葉系幹細胞集塊 Clumps of a MSC/ECM complexにおけるYAP/TAZシグナルの解析2017

    • Author(s)
      小松奈央、加治屋 幹人, 本池 総太, 竹脇 学, 竹下 慶, 岩田 倫幸, 應原 一久, 武田 克浩, 藤田 剛, 栗原 英見
    • Organizer
      第95回国際歯科研究学会
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 歯周病細胞治療における3次元培養法の現況と展望2017

    • Author(s)
      加治屋幹人
    • Organizer
      日本歯周病学会 60周年記念京都大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Clumps of MSCs/ECM complexを用いた新規歯周組織再生療法2016

    • Author(s)
      竹脇 学, 加治屋幹人, 竹下 慶, 小松奈央, 岩田倫幸, 武田克浩, 應原一久, 藤田 剛, 栗原英見
    • Organizer
      第145回日本歯科保存学会2016年度秋季学術大会
    • Place of Presentation
      松本市
    • Year and Date
      2016-10-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] 骨髄間葉系幹細胞集塊Clumps of a MSC/ECM complex (C-MSC)におけるメカノトランスダクションの解析2016

    • Author(s)
      小松奈央,加治屋幹人, 竹下 慶, 竹脇 学, 本池総太, 應原一久, 武田克浩, 岩田倫幸, 藤田 剛,栗原英見
    • Organizer
      第37回日本炎症・再生医学会
    • Place of Presentation
      京都市
    • Year and Date
      2016-06-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] 間葉系幹細胞と細胞外基質からなる細胞集塊を用いた新規の他家細胞移植療法の基礎研究2016

    • Author(s)
      竹下慶,加治屋幹人, 竹脇学、小松奈央、本池総太、藤田剛、栗原英見
    • Organizer
      第59回春季日本歯周病学会学術大会
    • Place of Presentation
      鹿児島市
    • Year and Date
      2016-05-20
    • Related Report
      2016 Annual Research Report
  • [Presentation] 凍結保存が間葉系幹細胞集塊Clumps of MSCs/ECM complexによる骨再生に及ぼす影響2016

    • Author(s)
      本池総太,加治屋 幹人, 竹下 慶、竹脇 学、岩田倫幸、武田克浩、應原一久、 藤田 剛、栗原英見
    • Organizer
      第59回春季日本歯周病学会学術大会
    • Place of Presentation
      鹿児島市
    • Year and Date
      2016-05-20
    • Related Report
      2016 Annual Research Report
  • [Presentation] 骨髄間葉系幹細胞集塊C-MSCを用いた新規細胞治療法の開発2016

    • Author(s)
      加治屋幹人
    • Organizer
      第59会春季日本歯周病学術大会
    • Place of Presentation
      鹿児島市
    • Year and Date
      2016-05-20
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 三次元培養で実現する有効・安全な組織再生技術の新展開2015

    • Author(s)
      加治屋幹人
    • Organizer
      日本組織培養学会第88回大会
    • Place of Presentation
      広仁会館(広島県広島市南区霞)
    • Year and Date
      2015-05-26
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] The development of immunomodulatory Clumps of a MSC/ECM complex for novel allograft cell transplantation therapy2015

    • Author(s)
      竹下慶、 加治屋 幹人、竹脇 学、小松 奈央、應原 一久、岩田倫幸、藤田 剛、柴 秀樹、栗原 英見
    • Organizer
      第58回春季歯周病学会
    • Place of Presentation
      幕張メッセ(千葉県千葉市美浜区中瀬)
    • Year and Date
      2015-05-14
    • Related Report
      2015 Annual Research Report
  • [Patent(Industrial Property Rights)] 凍結移植体及び凍結移植体の製造方法2016

    • Inventor(s)
      栗原英見、加治屋幹人、本池総太
    • Industrial Property Rights Holder
      広島大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-089188
    • Filing Date
      2016-04-27
    • Related Report
      2016 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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