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Study of sperm maturation factors on Asian Elephant

Research Project

Project/Area Number 15H05259
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Animal production science
Research InstitutionTokai University (2018-2019)
University of East Asia (2015-2017)

Principal Investigator

YOKO SATO  東海大学, 生物学部, 教授 (50398963)

Co-Investigator(Kenkyū-buntansha) 音井 威重  徳島大学, 大学院社会産業理工学研究部(生物資源産業学域), 教授 (30311814)
谷口 雅康  山口大学, 共同獣医学部, 准教授 (40625018)
Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
Fiscal Year 2018: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Keywords精子成熟 / 精子形成 / 熱ストレス / ゾウ / 停留精巣 / 温度 / 人工授精 / 精巣上体 / 細胞培養
Outline of Final Research Achievements

Elephant low quality of sperm may be caused by maturation process of epididymis, because the elephant sperm might receive the heat stress much longer than other mammals by their location. Here we investigated the expression of heat shock related molecules in elephant epididymis. HSF1 and HSP70 were quite highly expressed in the elephant epididymis compared to the other mammals, suggesting that there are endogenous heat stresses and heat responses in the epididymis. The localization area of these molecules was also different among elephants and the other animals. The heat shock responses, such as elevation of HSF1 and HSP70 were observed using tissue culture under the experimental heat stress, however, the timing of HSP70 response to the heat was delayed compared to the other animals. Because the regionalized epididymal fluid microenvironments are essential to the sperm maturation, the expression of these molecules by different way may affect the maturation process of elephant sperm.

Academic Significance and Societal Importance of the Research Achievements

本研究の特色は、これまで人工授精が不可能であったゾウから採取した精液でも人工授精が可能となる手法を提供するための基礎条件を検討するもので、今後、希少動物であるゾウの保護技術を確立する上での活用が期待される。さらに、ゾウ以外のヒトを含む他の哺乳動物においても、成熟精子形成能力が低下した希少品種および造精機能障害等の疾患に応用できる技術に繋がると思われる。

Report

(5 results)
  • 2019 Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (30 results)

All 2020 2019 2018 2017 2016 2015 Other

All Int'l Joint Research (5 results) Journal Article (11 results) (of which Int'l Joint Research: 10 results,  Peer Reviewed: 11 results,  Open Access: 3 results) Presentation (14 results) (of which Int'l Joint Research: 7 results,  Invited: 6 results)

  • [Int'l Joint Research] チュラロンコン大学/チェンマイ大学/カセサート大学(タイ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] チュラロンコン大学/チェンマイ大学/プリンスオブソンクラー大学(タイ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] チュラロンコン大学/カセサート大学/チェンマイ大学(タイ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Chulalongkorn University/Kasetsart University/Chiang Mai University(Thailand)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] University of Newcastle(Australia)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Abnormal Functions of Leydig Cells in Crossbred Cattle-Yak Showing Infertility2020

    • Author(s)
      Sato Y, Kuriwaki R, Hagino S, Shimazaki M, Sambuu R, Hirata M, Tanihara F, Takagi M, Taniguchi M, Otoi T.
    • Journal Title

      Reprod Domest Anim .

      Volume: 55 Issue: 2 Pages: 209-216

    • DOI

      10.1111/rda.13609

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A change in the steroid metabolic pathway in human testes showing deteriorated spermatogenesis2020

    • Author(s)
      Sato Yoko、Asahina Kiyoshi、Yoshiike Miki、Nozawa Shiari、Otoi Takeshige、Iwamoto Teruaki
    • Journal Title

      Reproductive Biology

      Volume: 20 Issue: 2 Pages: 210-219

    • DOI

      10.1016/j.repbio.2020.02.008

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effects of orvus es paste (OEP) on the viability of bull spermatozoa after double freezing and thawing.2018

    • Author(s)
      Shimazaki M, Urasako S, Tanaka M, Sato Y, Tanihara F, Hirata M, Taniguchi M, Takagi M, Otoi T
    • Journal Title

      Intern J Appl Res Vet Med

      Volume: 16 Pages: 32-38

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A new quantitative method for evaluating spermatogenesis of the testis hardness: A studying using a hamster model with experimental cryptorchidism.2018

    • Author(s)
      Ogawa K, Tomizuka D, Yoshiike M, Mozawa S, Nakazawa R, Sato Y, Morikawa Y, Iwamoto T, Ohnishi K
    • Journal Title

      日本コンピューター外科学会誌

      Volume: 20 Pages: 33-41

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chlorogenic acid supplementation during in vitro maturation improves maturation, fertilization, and developmental competence ofporcine oocytes.2017

    • Author(s)
      Nguyen TV, Tanihara F, Do L, Sato Y, Taniguchi M, Takagi M, Van Nguyen T, Otoi T
    • Journal Title

      Reprod Domest Anim.

      Volume: 52 Issue: 6 Pages: 969-975

    • DOI

      10.1111/rda.13005

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Epigenetic modulation on Cat-Cow interspecies somatic cell nuclear transfer embryos by treatment with Trichostatin A.2017

    • Author(s)
      Wittayarat, M., Sato, Y., Do, T.K.L., Chatdarong, K., Tharasanit, T., Techakumphu, M., Taniguchi, M. and Otoi, T.
    • Journal Title

      Anim. Sci. J.

      Volume: 88 Issue: 4 Pages: 593-601

    • DOI

      10.1111/asj.12676

    • NAID

      120007099360

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] CHARACTERISTICS AND FERTILITY OF SUMATRAN TIGER SPERMATOZOA CRYOPRESERVED WITH DIFFERENT SUGARS.2016

    • Author(s)
      Wayan Kurniani Karja N, Fahrudin M, Setiadi MA, Tumbelaka LI, Sudarwati R, Hastuti YT, Mulia BH, Widianti A, Sultan K, Terazono T, Namula Z, Taniguchi M, Tanihara F, Takemoto T, Kikuchi K, Sato Y, Otoi T.
    • Journal Title

      Cryoletters

      Volume: 37 Pages: 264-271

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effects of duration of electric pulse on in vitro development of cloned cat embryos with human artificial chromosome vector.2016

    • Author(s)
      Do L, Wittayarat M, Terazono T, Sato Y, Taniguchi M, Tanihara F, Takemoto T, Kazuki Y, Kazuki K, Oshimura M, Otoi T.
    • Journal Title

      Reprod Domest Anim

      Volume: 51 Issue: 6 Pages: 1039-1043

    • DOI

      10.1111/rda.12766

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] EFFECTS OF ORVUS ES PASTE ON THE MOTILITY AND VIABILITY OF YAK (BOS GRUNNIENS) EPIDIDYMAL AND EJACULATED SPERMATOZOA AFTER FREEZING AND THAWING.2015

    • Author(s)
      Shimazaki M, Sambuu R, Sato Y, Kim Do LT, Tanihara F, Taniguchi M, Otoi T.
    • Journal Title

      Cryo Letters.

      Volume: 36 Pages: 264-269

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Astaxanthin present in the maturation medium reduces negative effects of heat shock on the developmental competence of porcine oocytes.2015

    • Author(s)
      Do LT, Luu VV, Morita Y, Taniguchi M, Nii M, Peter AT, Otoi T.
    • Journal Title

      10.1016/j.repbio.2015.01.002.

      Volume: 15 Pages: 86-93

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] In vitro development of OPU-derived bovine embryos cultured either individually or in groups with the silk protein sericin and the viability of frozen-thawed embryos after transfer.2015

    • Author(s)
      Isobe T, Ikebata Y, Do LT, Tanihara F, Taniguchi M, Otoi T.
    • Journal Title

      Anim Sci J.

      Volume: 86 Issue: 7 Pages: 661-665

    • DOI

      10.1111/asj.12341

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 哺乳類停留精巣における精子形成と精子成熟2019

    • Author(s)
      佐藤 陽子
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 雑種雄性不稔は細胞サイズの調整機構の異常に起因するのか?2019

    • Author(s)
      佐藤 陽子
    • Organizer
      東海大学 2019年度研究交流会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 熱ストレスと造精機能障害について考える2019

    • Author(s)
      佐藤 陽子
    • Organizer
      東海大学マイクロ・ナノ啓発会第11回学術講演会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] CDC25A expression on the immunolocalization of Asian Elephant (Elephas maximus) testis.2018

    • Author(s)
      A. Sudsukh, Yoko Sato, T. Tharasanit
    • Organizer
      Annual meeting of Chulalonkorn University Veterinary Congress
    • Related Report
      2018 Annual Research Report
  • [Presentation] HSP90A may control spermatogenesis of Asian elephant (Elephas maximus) cryptorchid testes2018

    • Author(s)
      Y. Sato, T. Tharasanit, N. Tiptanavattana, A. Sudsukh, P. Phakdeedindan, C. Somgird, C. Thitaram, S. Mahasawangkul, M. Taniguchi, T. Otoi, M. Techakumphu.
    • Organizer
      The 13th international symposium on Spermatology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Unique expression of heat shock related molecules may control the spermatogenesis in Asian elephant (Elephas maxixus) cryptorchid testes.2017

    • Author(s)
      Sato Y, Tharasanit T, Tiptanavattana N, Thitaram C, Somgird C, Mahasawangkul S, Thongtip N, Taniguchi M, Otoi T, Techakumphu M.
    • Organizer
      4th World Congress of Reproductive Biology
    • Place of Presentation
      沖縄コンベンションセンター
    • Year and Date
      2017-09-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Evaluation of the Leydig cell function on crossbreeding yak showing infertility.2017

    • Author(s)
      Kuriwaki R, Sato Y, Hagino S, Shimazaki M, Samboo R, Do LTK, Tanihara F, Takagi M, Taniguchi M, Otoi T.
    • Organizer
      4th World Congress of Reproductive Biology
    • Place of Presentation
      沖縄コンベンションセンター
    • Year and Date
      2017-09-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ゾウ精子形成、精子成熟機構の解明をめざして2017

    • Author(s)
      佐藤陽子
    • Organizer
      日本アンドロロジー学会
    • Place of Presentation
      倉敷市芸文館
    • Year and Date
      2017-06-30
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ゾウ精子形成・精子成熟機構の解明をめざして2017

    • Author(s)
      佐藤陽子
    • Organizer
      日本アンドロロジー学会第36回学術大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] ロボット支援を用いた非閉塞性無精子症に対するMD-TESEの精子回収率改善を目指した挑戦的萌芽研究: 第2報 ハムスター精巣の硬さに影響する精巣内構造の変化に関する形態学的解析2017

    • Author(s)
      萩野詩季,栗脇良太,小川健司,冨塚大輔,吉池美紀,野澤資亜利,中澤龍斗,谷口雅康,高木光博,森川康英、大西公平,佐藤陽子,岩本晃明
    • Organizer
      日本アンドロロジー学会第36回学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Unique expression of heat shock related molecules may control the spermatogenesis in Asian elephant (Elephas maximus) cryptorchid testes2017

    • Author(s)
      Sato Y, Tharasanit T, Tiptanavattana N, Phakdeedindan P, Thitaram C, Somgird C, Mahasawangkul S, Thongtip N, Taniguchi M, Otoi T, Techakumphu M.
    • Organizer
      4th World Congress of Reproductive Biology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Evaluation of the Leydig cell function on crossbreeding yak showing infertility.2017

    • Author(s)
      R. Kuriwaki, Y. Sato, S. Hagino, M. Shimazaki, R. Sambuu, Do Lahn, F. Tanihara, M. Takagi, M. Taniguchi, T. Otoi
    • Organizer
      4th World Congress of Reproductive Biology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Unique expression patterns of heat shock related molecules in elephant testis and epididymis compared to those in other animal organs showing weak heat tolerance.2016

    • Author(s)
      Sato Y, Tharasanit T, Tiptanavattana N, Thitaram C, Somgird C, Mahasawangkul S, Thongtip N, Taniguchi M, Otoi T, Techakumphu M.
    • Organizer
      The 3rd seminar of priority universities for cooperation in Thailand
    • Place of Presentation
      Kasetsart Univ., Thailand
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular analysis of testicular cells in Asian elephant (Elephas maximus) cryptorchid testis.2015

    • Author(s)
      Sato Y., Tharasanit T., Tipanavattana N, Somgrid C, Thitaram C, Mahasawangkul S, Taniguchi M, Otoi T, Techakumphu M.
    • Organizer
      The 2nd Seminar of Priority University for Coorperation in Thailand between Chulalongkorn University, Kasetsart University and Yamaguchi University.
    • Place of Presentation
      Chulalongkorn University, Bangkok, Thailand.
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited

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Published: 2015-04-16   Modified: 2023-12-25  

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