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MEMS for electron microscopy to study nanobiology and its application to observation of bacterial dynamics

Research Project

Project/Area Number 15H05416
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Nano/Microsystems
Research InstitutionTokyo Institute of Technology

Principal Investigator

Ishida Tadashi  東京工業大学, 工学院, 准教授 (80517607)

Research Collaborator OHNO Teruaki  テクネックス工房, 技術者
KAKUSHIMA Kuniyuki  東京工業大学, 工学院, 准教授 (50401568)
OMATA Tatsuo  名古屋大学, 生命農学研究科, 教授 (50175270)
TAKATANI Nobuyuki  名古屋大学, 生命農学研究科, 研究員
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000)
Fiscal Year 2017: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2016: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2015: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
KeywordsMEMS / 電子顕微鏡 / 液体セル / 細菌 / 動態観察 / MEMS液体セル / マイクロ・ナノデバイス / ナノバイオ / マイクロ流路 / 培養チャンバ / マイクロナノデバイス
Outline of Final Research Achievements

Although specimens should normally be placed in vacuum condition for electron microscopes, we developed micro-electro-mechanical systems to visualize in-liquid bacteria. In this project, we developed a specimen holder to control cell cultural conditions, and a microfluidic device with a electron transparent membrane which separate vacuum and liquid. By using them, we visualized the dynamic motions of bubbles in liquid and droplets on the membrane, and observed cyanobacteria under cultural condition for long time.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (5 results)

All 2017 2016 Other

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (3 results) (of which Int'l Joint Research: 1 results) Remarks (1 results)

  • [Journal Article] In-situ realtime monitoring of nanoscale gold electroplating using micro-electro-mechanical systems liquid cell operating in transmission electron microscopy2016

    • Author(s)
      Minoru Egawa, Tadashi Ishida, Laurent Jalabert and Hiroyuki Fujita
    • Journal Title

      Applied Physics Letters

      Volume: 108 Issue: 2 Pages: 023104-023104

    • DOI

      10.1063/1.4939771

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Microfluidic Liquid Cell in Scanning Electron Microscope for Visualization of In-liquid Living Cell2017

    • Author(s)
      Tadashi Ishida
    • Organizer
      28th 2017 International Symposium on Micro-NanoMechatronics and Human Science
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] シアノバクテリアのマイクロ空間分裂抑制装置 ―局所的封入法の開発―2017

    • Author(s)
      石浦史也, 石田忠, 高谷信之, 小俣達男, 小俣透
    • Organizer
      ロボティクス・メカトロニクス講演会2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 多量のシアノバクテリアの一括分裂抑制マイクロ流体デバイス2016

    • Author(s)
      石田忠、近藤晃弘、高谷信之、小俣達男、小俣透
    • Organizer
      ロボティクス・メカトロニクス講演会2016 in Yokohama
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-06-08
    • Related Report
      2016 Annual Research Report
  • [Remarks] 研究室HP

    • URL

      http://www.olab.pms.titech.ac.jp/research/research_frame.html

    • Related Report
      2017 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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