The role of HDOI in the relationship between bone and fat.

• Project/Area Number: 15H05646
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: General physiology
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: Takano Haruko 国立研究開発法人国立循環器病研究センター, 研究所, 研究員 (40532891)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000); Fiscal Year 2017: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000); Fiscal Year 2016: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000); Fiscal Year 2015: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
• Keywords: 脂肪組織 / ナトリウム利尿ペプチド / 骨形成 / 機械刺激 / 骨膜 / 間葉系幹細胞 / 骨芽細胞 / 伸展刺激 / 生理学 / 運動負荷 / 肥満 / 骨 / ベージュ細胞

Outline of Final Research Achievements

Exercise-induced myokines provide systemic benefits by controlling adipose tissues. However, the relationships between bones and adipose tissues remain to be elucidated. In this study, we analyzed whether periosteum-derived HDOI regulates adipose tissues. After backcrossing to C57BL/6 for 10 generations, we didn’t find any abnormalities in adipose tissues of HDOI-KO mice compared to wild-type mice. Furthermore, HDOI-Tg mice were quite normal in their adipose tissues and showed no metabolic differences even under high-fat diet conditions. Although exercise didn’t induce HDOI directly, mechanical stimulation was required to maintain the expression of HDOI.

Research Products

• [Int'l Joint Research] KAIST(Korea)
• [Journal Article] TH2 response governed by Bcl6 function in naturally occurring memory phenotype CD4+ T cells. (2018)
  • Author(s): Takashi Ogasawara,Yuko Kohashi,Jun Ikari,Toshibumi Taniguchi,Nobuhide Tsuruoka,Haruko Watanabe-Takano,Lisa Fujimura,Akemi Sakamoto,Masahiko Hatano,Hirokuni Hirata,Yasutsugu Fukushima,Takeshi Fukuda,Kazuhiro Kurasawa,Koichiro Tatsumi,Takeshi Tokuhisa,Masafumi Arima
  • Journal Title: Front. Immunol. Volume: 9 Pages: 1-17
  • DOI: 10.3389/fimmu.2018.00750
  • Peer Reviewed / Open Access
• [Journal Article] A new secretory peptide of natriuretic peptide family, osteocrin, suppresses the progression of congestive heart failure after myocardial infarction. (2018)
  • Author(s): Miyazaki T, Otani K, Chiba A, Nishimura H, Tokudome T, Takano-Watanabe H, Matsuo A, Ishikawa H, Shimamoto K, Fukui H, Kanai Y, Yasoda A, Ogata S, Nishimura K, Minamino N, Mochizuki N.
  • Journal Title: Circulation Research Volume: 122(5) Pages: 742-751
  • DOI: 10.1161/circresaha.117.312624
  • Peer Reviewed / Open Access
• [Journal Article] Cardiomyokines from the heart. (2018)
  • Author(s): Chiba A, Watanabe-Takano H, Miyazaki T, Mochizuki N.
  • Journal Title: Cell Mol Life Sci. Volume: 75 Pages: 1349-1362
  • DOI: 10.1007/s00018-017-2723-6
  • Peer Reviewed / Open Access
• [Journal Article] Circulating osteocrin stimulates bone growth by limiting C-type natriuretic peptide clearance (2017)
  • Author(s): Kanai Y, Yasoda A, Mori KP, Watanabe-Takano H, Nagai-Okatani C, Yamashita Y, Hirota K, Ueda Y, Yamauchi I, Kondo E, Yamanaka S, Sakane Y, Nakao K, Fujii T, Yokoi H, Minamino N, Mukoyama M, Mochizuki N, Inagaki N.
  • Journal Title: J Clin Invest. Volume: 127 Pages: 4136-4147
  • DOI: 10.1172/jci94912
  • Peer Reviewed / Open Access
• [Journal Article] Development of chronic allergic responses by dampening Bcl6-mediated suppressor activity in memory T helper 2 cells (2017)
  • Author(s): Ogasawara T, Hatano M, Satake H, Ikari J, Taniguchi T, Tsuruoka N, Watanabe-Takano H, Fujimura L, Sakamoto A, Hirata H, Sugiyama K, Fukushima Y, Nakae S, Matsumoto K, Saito H, Fukuda T, Kurasawa K, Tatsumi K, Tokuhisa T, Arima M.
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 114
  • DOI: 10.1073/pnas.1613528114
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Osteocrin, a peptide secreted from the heart and other tissues, contributes to cranial osteogenesis and chondrogenesis in zebrafish (2017)
  • Author(s): Chiba A, Watanabe-Takano H, Terai K, Fukui H, Miyazaki T, Uemura M, Hashimoto H, Hibi M, Fukuhara S, Mochizuki N
  • Journal Title: Development. Volume: 144 Pages: 334-344
  • DOI: 10.1242/dev.143354
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Transcriptional repression of p27 is essential for murine embryonic development. (2016)
  • Author(s): Teratake Y, Kuga C, Hasegawa Y, Sato Y, Kitahashi M, Fujimura L, Watanabe-Takano H, Sakamoto A, Arima M, Tokuhisa T, Hatano M.
  • Journal Title: Sci Rep. Volume: 6 Pages: 26244-26244
  • DOI: 10.1038/srep26244
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] DA-Raf-mediated suppression of the Ras-ERK pathway is essential for TGF-β1-induced epithelial-mesenchymal transition in alveolar epithelial type 2 cells (2015)
  • Author(s): Watanabe-Takano, H., Takano, K., Hatano, M., Tokuhisa, T. and Endo T.
  • Journal Title: PLoS One Volume: 10
  • DOI: 10.1371/journal.pone.0127888
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Osteocrinは骨形態形成を制御する骨膜ホルモンである (2017)
  • Author(s): 高野 晴子
  • Organizer: 第35回日本骨代謝学会学術集会
• [Presentation] Osteocrinは骨形態形成を制御する骨膜ホルモンである (2017)
  • Author(s): 高野 晴子
  • Organizer: 第69回日本細胞生物学会大会
• [Presentation] Osteocrinは骨形態形成を制御する骨膜ホルモンである (2016)
  • Author(s): 高野晴子、千葉彩乃、宮崎敬大、金井有吾、森慶太、八十田明宏、横井秀基、 望月直樹
  • Organizer: 第39回日本分子細胞生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-11-30
• [Presentation] DA-Raf-mediated Suppression of the Ras-ERK Pathway Is Essential for TGF-b-induced Epithelial-mesenchymal Transition in Alveolar Epithelial Type 2 Cells (2015)
  • Author(s): Haruko Watanabe-Takano
  • Organizer: The American Society for Cell Biology
  • Place of Presentation: San Diego
  • Year and Date: 2015-12-14
  • Int'l Joint Research
• [Book] THE LUNG perspective (2015)
  • Author(s): 遠藤 剛、渡辺ー高野 晴子、高野和儀
  • Total Pages: 8
  • Publisher: メディカルレビュー社
• [Book] 血管医学 (2015)
  • Author(s): 高野 晴子、千葉 彩乃、宮崎 敬大、望月 直樹
  • Total Pages: 3
  • Publisher: メディカルレビュー社
• [Remarks] 国立循環器病研究センター研究所 細胞生物学部 高野 晴子
  • URL: http://www.ncvc.go.jp/res/divisions/structural_analysis/takano.html