Proposal of a novel therapy for denture ulcer applying combination of polyphenol and disinfection technique with hydrogen peroxide photolysis

• Project/Area Number: 15H06044
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Tohoku University
• Principal Investigator: KURAUCHI MICHIKO 東北大学, 大学病院, 医員 (00757263)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ポリフェノール / 酸化ストレス

Outline of Final Research Achievements

Caffeic acid (CA) and chlorogenic acid (ChA), both of which accelerated proliferation of fibroblasts by short-term treatment, exerted cytoprotected effect on human gingival fibroblast (hGFs) exposed to various harsh and oxidative stressors. In addition, 1-min pretreatment with CA and ChA reduced an inflammatory cytokine TNF-αproduction by human monocyte cell line THP-1 stimulated with LPS derived from Porphyromonas gingivalis, a major causative bacterial species of periodontitis. The results suggest that these polyphenols could be adjunctive agents for disinfection by H2O2 photolysis via cytoprotective and anti-inflammatory effect on ulcer lesion.