Kinetic analysis of the activation process of receptor tyrosine kinases by using DNA aptamer

• Project/Area Number: 15H06136
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Bio-related chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: Ueki Ryosuke 東京大学, 大学院工学系研究科(工学部), 助教 (90755703)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: DNAアプタマー / チロシンキナーゼ受容体 / アプタマー / 受容体 / 核酸 / 増殖因子 / 受容体型チロシンキナーゼ / 核酸アプタマー

Outline of Final Research Achievements

Receptor tyrosine kinases (RTKs) are known as receptors for growth factors. In this research, we tried to develop novel molecular tools that can evaluate clustering behavior of RTKs on cell surface in order to get a deeper insight into the activation mechanism of RTKs at a molecular level. We designed some DNA aptamer-based functional ligands and applied them in regulation of clustering behavior of RTKs. For analysis of RTK clustering, we focused on super-resolution imaging methods. We constructed some plasmid vectors and tested them in transfection experiments for the imaging analysis.

Research Products

• [Presentation] Functional oligonucleotide for regulation of cellular activities (1): Oligonucleotide-based growth factor mimetics -Regulation of cellular functions and its application- (2017)
  • Author(s): Ryosuke Ueki, Naoto Kanda, Satoshi Uchida, Horacio Cabral, Shinsuke Sando
  • Organizer: 日本化学会第97春期年会
  • Place of Presentation: 慶應義塾大学日吉キャンパス（神奈川）
  • Year and Date: 2017-03-16
• [Presentation] 機能性核酸に基づく細胞機能制御（２） 増殖因子シグナルの外部環境依存的スイッチング (2017)
  • Author(s): 熱田早紀、植木亮介、山東信介
  • Organizer: 日本化学会第97春期年会
  • Place of Presentation: 慶應義塾大学日吉キャンパス（神奈川）
  • Year and Date: 2017-03-16
• [Presentation] Synthetic Growth Factors based on Functional Oligonucleotides (2016)
  • Author(s): Ryosuke Ueki, Shinsuke Sando
  • Organizer: The 43th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 熊本大学黒髪キャンパス（熊本）
  • Year and Date: 2016-09-27
  • Int'l Joint Research
• [Presentation] DNA nanotechnology for controlling receptor signaling (2015)
  • Author(s): Ryosuke Ueki
  • Organizer: The International Chemical Congress of Pacific Basin Societies 2015
  • Place of Presentation: ハワイ・コンベンション・センター（ハワイ、米国）
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Effect of DNA aptamer-based functional ligands on cellular motility (2015)
  • Author(s): Ryosuke Ueki
  • Organizer: The 42th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: あいめっせホール（姫路市、兵庫県）
  • Year and Date: 2015-09-23
  • Int'l Joint Research