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The periodontal regeneration therapy with novel amelogenin binding protein Grp78

Research Project

Project/Area Number 15H06492
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Periodontology
Research InstitutionKyushu University

Principal Investigator

Toyoda Kyosuke  九州大学, 歯学研究院, 助教 (70757947)

Project Period (FY) 2015-08-28 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsアメロジェニン / Grp78
Outline of Final Research Achievements

Our recent proteomic study identified glucose-regulated protein 78 (Grp78) as a new amelogenin-binding protein. we demonstrate the cellular responses induced by the biological interaction between amelogenin and Grp78 in the human undifferentiated PDL cell line 1-17, which possesses the most typical characteristics of PDLSCs. Microarray analysis indicated that rM180 and Grp78 regulate the expression profiles of cell migration-associated genes in 1-17 cells. Moreover, Grp78 overexpression enhanced rM180-induced cell migration and adhesion without affecting cell proliferation, while silencing of Grp78 diminished these activities. Finally, binding of rM180 to Grp78 promoted the formation of lamellipodia, and the simultaneous activation of Rac1 was also demonstrated by NSC23766, a widely accepted Rac1 inhibitor. These results suggest that Grp78 is essential for enhancing amelogenin-induced migration in 1-17 cells.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • Research Products

    (14 results)

All 2016 2015

All Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (11 results) (of which Int'l Joint Research: 6 results)

  • [Journal Article] Grp78 is critical for amelogenin-induced cell migration in a multipotent clonal human periodontal ligament cell line.2016

    • Author(s)
      Toyoda K, Fukuda T, Sanui T*, Tanaka U, Yamamichi K, Atomura R, Maeda H, Tomokiyo A, Taketomi T, Uchiumi T, Nishimura F
    • Journal Title

      Journal of Cellular Physiology

      Volume: 231 Issue: 2 Pages: 414-427

    • DOI

      10.1002/jcp.25087

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Inhibition of Sprouty2 polarizes macrophages toward an M2 phenotype by stimulation with interferon γ and Porphyromonas gingivalis lipopolysaccharide.2016

    • Author(s)
      Atomura R, Sanui T*, Fukuda T, Tanaka U, Toyoda K, Taketomi T, Yamamichi K, Akiyama H, Nishimura F
    • Journal Title

      Immunity, Inflammation and Disease

      Volume: 4(1) Issue: 1 Pages: 98-110

    • DOI

      10.1002/iid3.99

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Sprouty2 inhibition promotes proliferation and migration of periodontal ligament cells.2015

    • Author(s)
      Tanaka U, Sanui T*, Fukuda T, Toyoda K, Atomura R, Yamamichi K, Maeda H, Nishimura F
    • Journal Title

      Oral Diseases

      Volume: 21 Issue: 8 Pages: 977-986

    • DOI

      10.1111/odi.12369

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Spry2 Downregulation Shifts Macrophages Toward an M2 Phenotype by Stimulation with Interferon γ and Porphyromonas gingivalis Lipopolysaccharide.2016

    • Author(s)
      Terukazu Sanui, Hajime Akiyama, Takao Fukuda, Urara Tanaka, Kyosuke Toyoda, Takaharu Taketomi, Fusanori Nishimura
    • Organizer
      4th General Session & Exhibition of the International Association for Dental Research
    • Place of Presentation
      COEX Convention & Exbition Center Seoul, Republic of Korea
    • Year and Date
      2016-06-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Grp78 is crucial for cell migration induced by amelogenin in a human periodontal ligament cells.2016

    • Author(s)
      Kyosuke Toyoda, Hajime Akiyama, Takao Fukuda, Terukazu Sanui, Urara Tanaka, Takaharu Taketomi, Fusanori Nishimura
    • Organizer
      4th General Session & Exhibition of the International Association for Dental Research
    • Place of Presentation
      COEX Convention & Exbition Center Seoul, Republic of Korea
    • Year and Date
      2016-06-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Amelogenin Modulates Macrophage Phenotype During Inflammatory Responses.2016

    • Author(s)
      Kensuke Yamamichi, Hajime Akiyama, Takao Fukuda, Terukazu Sanui, Kyosuke Toyoda, Urara Tanaka, Takaharu Taketomi, Fusanori Nishimura
    • Organizer
      4th General Session & Exhibition of the International Association for Dental Research
    • Place of Presentation
      COEX Convention & Exbition Center Seoul, Republic of Korea
    • Year and Date
      2016-06-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Spry2 knockdown enhances proliferation and migration but reduces differentiation of periodontal ligament cells through bFGF and EGF stimulation.2016

    • Author(s)
      Hajime Akiyama, Urara Tanaka, Terukazu Sanui, Takao Fukuda, Kyosuke Toyoda, Kensuke Yamamichi, Takaharu Taketomi, Fusanori Nishimura
    • Organizer
      4th General Session & Exhibition of the International Association for Dental Research
    • Place of Presentation
      COEX Convention & Exbition Center Seoul, Republic of Korea
    • Year and Date
      2016-06-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Molecular basis of amelogenin-induced periodontal tissue regeneration2016

    • Author(s)
      Takao Fukuda, Terukazu Sanui, Kyosuke Toyoda, Urara Tanaka, Kensuke Yamamichi, Ryo Atomura, Hajime Akiyama, Fusanori Nishimura
    • Organizer
      口腔から健康長寿を支えるプロジェクト推進に向けた研究拠点構築プログラム2nd Symposium
    • Place of Presentation
      福岡リーセントホテル
    • Year and Date
      2016-02-28
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] アメロジェニンが有する抗炎症効果の機序解明2016

    • Author(s)
      山道研介、福田隆男、讃井彰一、豊田敬介、田中麗、西村英紀
    • Organizer
      平成28年度日本歯周病学会九州五大学・日本臨床歯周病学会九州支部・合同研修会
    • Place of Presentation
      長崎県歯科医師会館、長崎市
    • Related Report
      2016 Annual Research Report
  • [Presentation] アメロジェニンが炎症過程におけるマクロファージの形質転換におよぼす影響2015

    • Author(s)
      山道研介、福田隆男、讃井彰一、豊田敬介、後村亮、西村英紀
    • Organizer
      日本歯科保存学会 2015年度秋季学術大会 (143回) 第17回日韓歯科保存学会学術大会
    • Place of Presentation
      文京シビックホール
    • Year and Date
      2015-11-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヒト歯根膜幹細胞/前駆細胞株の細胞遊走時に及ぼす分子機構2015

    • Author(s)
      豊田敬介、福田隆男、讃井彰一、山道研介、後村亮、西村英紀
    • Organizer
      日本歯科保存学会 2015年度秋季学術大会 (143回) 第17回日韓歯科保存学会学術大会
    • Place of Presentation
      文京シビックホール
    • Year and Date
      2015-11-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] Spry2抑制によるM1マクロファージへの分化抑制メカニズムの解析2015

    • Author(s)
      後村亮、讃井彰一、福田隆男、豊田敬介、山道研介、秋山元、西村英紀
    • Organizer
      日本歯科保存学会 2015年度秋季学術大会 (143回) 第17回日韓歯科保存学会学術大会
    • Place of Presentation
      文京シビックホール
    • Year and Date
      2015-11-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] アメロジェニンが有する抗炎症効果の機序解明2015

    • Author(s)
      山道研介、福田隆男、讃井彰一、豊田敬介、後村亮、秋山元、田中麗、西村英紀
    • Organizer
      平成27年度日本臨床歯周病学会九州支部・日本歯周病学会九州五大学合同研修会
    • Place of Presentation
      アクロス福岡
    • Year and Date
      2015-11-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] Grp78 Is Critical For Amelogenin-induced Cell Migration In PDLSCs2015

    • Author(s)
      Takao Fukuda, Kyosuke Toyoda, Terukazu Sanui, Urara Tanaka, Kensuke Yamamichi, Ryo Atomura, Takaharu Taketomi, Fusanori Nishimura
    • Organizer
      The 63rd Annual Meeting of Japanese Association for Dental Research
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2015-10-30
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research

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Published: 2015-08-26   Modified: 2018-03-22  

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