| Project/Area Number |
15K00541
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Nagoya University |
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Principal Investigator |
KARATA Kiyonobu 名古屋大学, 環境医学研究所, 研究機関研究員 (90345017)
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| Co-Investigator(Kenkyū-buntansha) |
荻 朋男 名古屋大学, 環境医学研究所, 教授 (80508317)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 塩基除去修復 / RNAポリメラーゼ / 転写共役型ヌクレオチド除去修復 / RNA polymerase II |
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| Outline of Final Research Achievements |
The detailed function of TC-NER-specific factors, CSA, CSB and UVSSA, remains to be defined. In order to elucidate their function biochemically, we tried to reconstitute in vitro TC-NER assay system using DNA templates harboring a site-directed DNA damage. In addition, we have developed the purification method of UVSSA protein for its X-ray crystal structure analysis.
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