| Project/Area Number |
15K00590
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Modeling and technologies for environmental conservation and remediation
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| Research Institution | Kyoto Gakuen University (2016-2017)
Yamagata University (2015) |
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Principal Investigator |
Sakurama Haruko 京都学園大学, バイオ環境学部, 講師 (90456426)
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| Co-Investigator(Kenkyū-buntansha) |
高塚 由美子 山形大学, 大学院理工学研究科, 助教 (70570810)
乾 秀之 神戸大学, バイオシグナル総合研究センター, 准教授 (90314509)
原 富次郎 山形大学, 大学院理工学研究科, 教授 (70616193)
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| Project Period (FY) |
2015-10-21 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ポリ塩化ビフェニル / 芳香環水酸化ジオキシゲナーゼ / 環境浄化 |
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| Outline of Final Research Achievements |
Polychlorinated biphenyls (PCBs) have been recognized as serious environmental pollutants on a global scale. In order to obtain novel aromatic-ring hydroxylating dioxygenases that can degrade on more highly chlorinated PCBs congeners, we engineered the aromatic-ring hydroxylating dioxygenase from Commamonas by a site-directed mutagenesis, and have successfully obtained a few mutants with extended substrate specificity. In addition, we have established the expression system of the two genes of the aromatic-ring hydroxylating dioxygenase with distinct substrate specificities.
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