Creation of novel aromatic-ring hydroxylating dioxygenase with extended substrate specificity toward polychlorinated biphenyls
Project/Area Number |
15K00590
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Modeling and technologies for environmental conservation and remediation
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Research Institution | Kyoto Gakuen University (2016-2017) Yamagata University (2015) |
Principal Investigator |
Sakurama Haruko 京都学園大学, バイオ環境学部, 講師 (90456426)
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Co-Investigator(Kenkyū-buntansha) |
高塚 由美子 山形大学, 大学院理工学研究科, 助教 (70570810)
乾 秀之 神戸大学, バイオシグナル総合研究センター, 准教授 (90314509)
原 富次郎 山形大学, 大学院理工学研究科, 教授 (70616193)
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Project Period (FY) |
2015-10-21 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | ポリ塩化ビフェニル / 芳香環水酸化ジオキシゲナーゼ / 環境浄化 |
Outline of Final Research Achievements |
Polychlorinated biphenyls (PCBs) have been recognized as serious environmental pollutants on a global scale. In order to obtain novel aromatic-ring hydroxylating dioxygenases that can degrade on more highly chlorinated PCBs congeners, we engineered the aromatic-ring hydroxylating dioxygenase from Commamonas by a site-directed mutagenesis, and have successfully obtained a few mutants with extended substrate specificity. In addition, we have established the expression system of the two genes of the aromatic-ring hydroxylating dioxygenase with distinct substrate specificities.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Molecular insights into the role of a distal F240A mutation that alters CYP1A1 activity towards persistent organic pollutants2017
Author(s)
Navratilova, V., Paloncyoya, M., Berka, K., Mise, S., Haga, Y., Matsumura, C., Sakaki, T., Inui, H., Otyepka, M.
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Journal Title
Biochimica et Biophysica Acta General Subjects
Volume: 1861
Issue: 11
Pages: 2852-2860
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Structural determinants for the position of 2,3’,4,4’,5-pentachlorobiphenyl (CB118) hydroxylation by mammalian cytochrome P450 monooxygenases2016
Author(s)
Mise, S., Haga, Y., Itoh, T., Kato, A., Fukuda, I., Goto, E., Yamamoto, K., Yabu, M., Matsumura, C., Nakano. T., Sakaki, T., and Inui, H.
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Journal Title
Toxicological Sciences
Volume: 152
Issue: 2
Pages: 340-348
DOI
Related Report
Peer Reviewed
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[Presentation] Structural basis of CB118 metabolism by bacterial cytochrome P450 monooxygenase and its mutants with perfluorocarboxylic acids2017
Author(s)
Erika Goto, Yuki Haga, Makoto Kubo, Toshimasa Itoh, Chie Kasai, Osami Shoji, Keiko Yamamoto, Chisato Matsumura, Takeshi Nakano, Hideyuki Inui
Organizer
37th International Symposium on Halogenated Persistent Organic Pollutants, Vancouver, Canada
Related Report
Int'l Joint Research
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[Presentation] Metabolism of chiral polychlorinated biphenyls by mammalian cytochrome P450 monooxygenases2017
Author(s)
Terushi Ito, Chiharu Miwa, Yuki Haga, Erika Goto, Toshimasa Itoh, Keiko Yamamoto, Shintaro Mise, Chisato Matsumura, Takeshi Nakano, Hideyuki Inui
Organizer
37th International Symposium on Halogenated Persistent Organic Pollutants, Vancouver, Canada
Related Report
Int'l Joint Research
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[Presentation] Possible metabolic fates of polychlorinated biphenyls by soil bacterium under complex pollution and its structural basis2017
Author(s)
Erika Goto, Yuki Haga, Makoto Kubo, Toshimasa Itoh, Chie Kasai, Osami Shoji, Keiko Yamamoto, Chisato Matsumura, Takeshi Nakano, Hideyuki Inui
Organizer
The 14th International Symposium on Persistent Toxic Substances, Nagoya
Related Report
Int'l Joint Research
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[Presentation] Structural basis of CB118 metabolism by cytochrome P450 monooxygenase from Bacillus megaterium with decoy molecules2016
Author(s)
Erika Goto, Yuki Haga, Makoto Kubo, Toshimasa Itoh, Chie Kasai, Osami Shoji, Keiko Yamamoto, Chisato Matsumura, Takeshi Nakano, Hideyuki Inui
Organizer
The 13th International Symposium on Cytochrome P450 Biodiversity & Biotechnology
Place of Presentation
Vancouver, Canada
Year and Date
2016-07-22
Related Report
Int'l Joint Research
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