Design of a next generation thermoresponsive culture system for creating functional cultured hepatic sheet tissues
Project/Area Number |
15K01317
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biomedical engineering/Biomaterial science and engineering
|
Research Institution | Tokyo Women's Medical University |
Principal Investigator |
Kobayashi Jun 東京女子医科大学, 医学部, 講師 (20385404)
|
Co-Investigator(Renkei-kenkyūsha) |
AKIYAMA Yoshikatsu 東京女子医科大学, 医学部, 講師 (20349640)
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
Keywords | 生体材料 / 再生医療 / 組織工学 / 温度応答性高分子 / ヘパリン / 肝細胞 / ハイドロゲル / マイクロ流体 |
Outline of Final Research Achievements |
In order to prepare transplantable hepatocyte sheets with maintaining hepatic functions, a thermoresponsive culture system mimicking biological environments was designed. Transplantable hepatocyte sheets could be prepared while maintaining hepatic functions on a heparin-immobilized thermoresponsive surface containing a component of basement membrane such as growth factors. It was also possible to quantitatively evaluate the temperature-dependent affinity between cellular receptor/growth factor/the heparin-immobilized thermoresponsive surfaces. By using flexible basal substrate and microfluidic devices, more advanced hepatocyte sheet tissue culture system would be constructed.
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Report
(4 results)
Research Products
(12 results)