Analysis of mechanisms of autonomous function between the brainstem and the spinal cord with using a decerebrated and arterially perfused in situ preparation
| Project/Area Number |
15K01393
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Rehabilitation science/Welfare engineering
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| Research Institution | Hoshi University |
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Principal Investigator |
Yazawa Itaru 星薬科大学, 先端生命科学研究所, 客員研究員 (40360656)
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| Co-Investigator(Kenkyū-buntansha) |
塩田 清二 星薬科大学, 先端生命科学研究所, 教授 (80102375)
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | Autonomous function / Perfused preparation / Spinal cord / Locomotion / Brainstem / Respiration / Microglia / LPG / Spinal Cord / Entrainment / hyperoxia/normocapnea / Circulation / 除皮質灌流標本 |
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| Outline of Final Research Achievements |
Due to various reasons, it did not lead the completion a new perfusion system in which artificial blood was introduced to the perfused preparation. However, here, we report “involvement of microglia in the formation of functions produced by neural networks in the central nervous system (CNS)” which is one of the studies conducted in parallel with the research subjects.
It is known that microglia are poised to play important roles in shaping the developing CNS and contributing to overall nervous system function through a lifetime. We applied the current perfusion system to mice lacking microglia associated with interferon regulatory factor 8 and electrophysiologically examined postnatal changes in neural output generated from the neural network of the lower spinal cord that constitutes LGP. As a result, it was shown that this microglial defect might inhibit the normal maturation of the function generated by the neural network of the lower spinal cord constituting LPG.
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| Academic Significance and Societal Importance of the Research Achievements |
今回の研究成果の学術的意義は、①生後間もない時期～成体期の齧歯類に本方法論が適用できることから本方法論を用いてさまざまな分野の研究活動が行えること、②IRF8に関連するミクログリアの欠損が下位脊髄神経回路網で産生される運動機能の成熟を阻害する可能性があること、③今回の研究で使用した遺伝子改変動物は慢性骨髄性白血病の疾患モデルであることから慢性骨髄性白血病患者の下位脊髄神経回路網から産生される機能が未成熟である可能性があること、などが挙げられる。
社会的意義は、免疫不全により中枢神経障害や運動障害が引き起こされる可能性があるため、まずは、免疫と神経には関連性があることが認知されることである。
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Report
(5 results)
Research Products
(19 results)
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[Presentation] Significance of astrocytic activation in hypoxic respiratory responsiveness in the in vitro medulla-spinal cord preparation of the newborn rat.2018
Author(s)
I. Fukushi, M. Uchiyama, Y. Kurita, I. Yazawa, S. Okazaki, Y. Hasebe, Y. Kono, S. Yokota, K. Takeda, Y. Mori, H. Onimaru, Y. Okada.
Organizer
Society for Neuroscience 2018 Annual Meeting（San Diego, USA） H30年11月
Related Report
Int'l Joint Research
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[Presentation] 第94回日本生理学会大会2017
Author(s)
Y. Okada, I. Yazawa, K. Takeda, S. Okazaki, Y. Tamura, H. Onimaru
Organizer
Respiratory activities of medullary neurons and astrocytes in the isolated brainstem-spinal cord analyzed by Ca imaging
Place of Presentation
浜松
Year and Date
2017-03-28
Related Report
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[Presentation] A novel model of respiratory rhythm generation: a mechanism by interaction of intrinsically oscillating astrocytes and neurons2015
Author(s)
Y. Okada, Y. Oku, T. Sasaki, C. Vivar, S. Yokota, K. Takeda, I. Fukushi, I. Yazawa, H. Someya, Y. Tamura
Organizer
Society for Neuroscience 2015 Annual Meeting
Place of Presentation
Chicago, Ill, USA
Year and Date
2015-10-17
Related Report
Int'l Joint Research
