Analysis of bone metabolism under hyperglycemia using unique type 2 diabetes model and proposal of improvement method for bone metabolism in type 2 diabetes by exercise.

• Project/Area Number: 15K01705
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied health science
• Research Institution: Kanazawa University
• Principal Investigator: KITAMURA Kei-ichiro 金沢大学, 保健学系, 教授 (80283117)
• Co-Investigator(Kenkyū-buntansha): 鈴木 信雄 金沢大学, 環日本海域環境研究センター, 教授 (60242476) ; 池亀 美華 岡山大学, 医歯薬学総合研究科, 准教授 (70282986)
• Co-Investigator(Renkei-kenkyūsha): HONDA Masao 金沢大学, 保健学系, 教授 (00272980)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 糖尿病 / キンギョ / 高でんぷん含有飼料 / 骨代謝 / 再生ウロコ / コラーゲン線維 / 糖化 / 運動 / エキソーム解析

Outline of Final Research Achievements

We used fish, characterized by a prolonged hour hyperglycemia after starch-rich feeding, as a unique model of type 2 DM. In this model, the interfibrous crosslinking caused by production of advanced glycation end products accompanying the glycation of type I collagen fiber was increased. Furthermore, analysis of three-points bending test of glycated type I collagen fiber demonstrated that the increment of interfibrous crosslinking of collagen fibers leads to decrease of collagen fiber elasticity and of bending strength of collagen fiber. Our results indicated that the glycation of bone structure proteins bring increment of fracture risk.

Research Products

• [Journal Article] a-melanocyte-stimulating hormone promotes the bone resorption resulting from increased osteoblastic and osteoclastic activities in goldfish. (2018)
  • Author(s): Ishizu, H., Sekiguchi, T., Ikari, T.,, Kitamura, K., Kitani, Y., Endo, M., Urata, M., Kinoshita, Y., Hattori, A., Srivastav, A.K., Mishima, H., Mizusawa, K., Takahashi, A. and Suzuki, N.
  • Journal Title: Gen. Comp. Endocrinol. Volume: 262 Pages: 99-105
  • DOI: 10.1016/j.ygcen.2018.03.020
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effects of hyperglycemia on bone metabolism and bone matrix in goldfish scales. (2017)
  • Author(s): Kitamura K, Andoh T, Okesaku W, Tazaki Y, Ogai K, Sugitani K, Kobayashi I, Suzuki N, Chen W, Ikegame M, Hattori A
  • Journal Title: Comparative Biochemistry and Physiology, Part A Volume: 203 Pages: 152-158
  • DOI: 10.1016/j.cbpa.2016.09.010
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] <b>Effects of low-intensity pulsed ultrasound on osteoclasts: Analysis with goldfish scales as a model of </b><b>bone </b> (2017)
  • Author(s): Hanmoto T, Tabuchi Y, Ikegame M, Kondo T, Kitamura KI, Endo M, Kobayashi I, Mishima H, Sekiguchi T, Urata M, Seki A, Yano S, Hattori A, Suzuki N.
  • Journal Title: Biomedical Research Volume: 38 Issue: 1 Pages: 71-77
  • DOI: 10.2220/biomedres.38.71
  • NAID: 130005242919
  • ISSN: 0388-6107, 1880-313X
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Sardine procalcitonin amino-terminal cleavage peptide has a different action from calcitonin and promotes osteoblastic activity in the scales of goldfish. (2017)
  • Author(s): Kase, Y., Ikari, T., Sekiguchi, T., Sato, M., Ogiso, S., Kawada, T., Matsubara, S., Satake, H., Sasayama, Y., Endo, M., Kitamura, K., Hattori, A., Watanabe, T.X., Maruyama, Y., Watanabe, Y., Funahashi, H., Kambegawa, A. and Suzuki, N.
  • Journal Title: Comp. Biochem. Physiol. Part A Volume: 211 Pages: 73-83
  • DOI: 10.1016/j.cbpa.2017.06.007
  • NAID: 120006336026
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Is vaccenic acid (18:1t n-7) associated with an increased incidence of hip fracture? An explanation for the calcium paradox. (2016)
  • Author(s): Hamazaki, K., Suzuki, N., Kitamura, K., Hattori, A., Nagasawa, T., Itomura, M., and Hamazaki, T.
  • Journal Title: Prostaglandins Leukot. Essent. Fatty Acids Volume: 109 Pages: 8-12
  • DOI: 10.1016/j.plefa.2016.04.001
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Fish scale is a suitable model for analyzing determinants of skeletal fragility in type 2 diabetes. (2016)
  • Author(s): Suzuki N, Kitamura K, Hattori A
  • Journal Title: Endocrine Volume: 54 Issue: 3 Pages: 575-577
  • DOI: 10.1007/s12020-016-1153-9
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A detailed protocol for perspiration monitoring using a novel, small, wireless device. (2016)
  • Author(s): Ogai K, Fukuoka M, Kitamura K, Uchide K, Nemoto T
  • Journal Title: J Vis Exp Volume: 24 Issue: 117
  • DOI: 10.3791/54837
  • Peer Reviewed / Open Access
• [Journal Article] Long-term Measurement of Maternal Pulse Rate Dynamics Using a Home-based Sleep Monitoring System. (2016)
  • Author(s): Chen Y., Chen W, Kitamura K., Nemoto T
  • Journal Title: Journal of Sensors Volume: in press Pages: 1-11
  • DOI: 10.1155/2016/5730142
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Development of a small wireless device for perspiration monitoring. (2016)
  • Author(s): Ogai K, Fukuoka M, Kitamura K, Uchide K, Nemoto T
  • Journal Title: Med Eng Phys. Volume: 38 Issue: 4 Pages: 391-397
  • DOI: 10.1016/j.medengphy.2015.12.009
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Low-intensity pulsed ultrasound induces apoptosis in osteoclasts: Fish scales are a suitable model for the analysis of bone metabolism by ultrasound. (2016)
  • Author(s): Suzuki N, Hanmoto T, Yano S, Furusawa Y, Ikegame M, Tabuchi Y, Kondo T, Kitamura K, Endo M, Yamamoto T, Sekiguchi T, Urata M, Mikuni-Takagaki Y, Hattori A.
  • Journal Title: Comp. Biochem. Physiol. Part A Volume: 195 Pages: 26-31
  • DOI: 10.1016/j.cbpa.2016.01.022
  • NAID: 120005743920
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Increased level of tumor necrosis factor-alpha (TNF-α) on the skin of Japanese obese males: measured by quantitative skin blotting. (2016)
  • Author(s): Ogai K, Matsumoto M, Aoki M, Minematsu T, Kitamura K, Kobayashi M, Sanada H and Sugama J
  • Journal Title: Int J Cosmet Sci. Volume: in press Issue: 5 Pages: 462-469
  • DOI: 10.1111/ics.12312
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Is vaccenic acid (18:1t n-7) associated with an increased incidence of hip fracture? An explanation for the calcium paradox. (2016)
  • Author(s): Hamazaki K., Suzuki N., Kitamura K., Hattori A., Nagasawa T., Itomura M., Hamazaki T.
  • Journal Title: Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA) Volume: in press
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Monohydroxylated polycyclic aromatic hydrocarbon influences spicule formation in the early development of sea urchins (Hemicentrotus pulcherrimus). (2015)
  • Author(s): Suzuki, N., Ogiso, S., Yachiguchi, K., Kawabe, K., Makino, F., Toriba, A., Kiyomoto, M., Sekiguchi, T., Tabuchi, Y., Kondo, T., Kitamura, K., Hong, C., Srivastav, A.K., Oshima, Y., Hattori, A., Hayakawa, K.
  • Journal Title: Comp Biochem Physiol C Toxicol Pharmacol. Volume: 171 Pages: 55-60
  • DOI: 10.1016/j.cbpa.2015.02.004
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A basic study for prediction of food hardness based on an acceleration monitoring of lower jaw during mastication. (2015)
  • Author(s): Ogai K, Kitamura K, Nemoto T.
  • Journal Title: Journal of Nursing Science and Engineering Volume: 2 Pages: 9-14
  • NAID: 130006406473
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Development of an improved method for quantitative analysis of skin blotting: Increasing reliability and applicability for skin assessment. (2015)
  • Author(s): Ogai K, Matsumoto M, Minematsu H, Kitamura K, Kobayashi M, Sugama J and Sanada H
  • Journal Title: International Journal of Cosmetic Science Volume: - Issue: 4 Pages: 425-432
  • DOI: 10.1111/ics.12217
  • NAID: 120005704522
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Novel tryptophan derivatives as potentially effective therapeutic drugs to treat bone diseases. (2015)
  • Author(s): Suzuki, N., Somei, M., Seki, A., Sekiguchi, T., Tabuchi, Y., Mishima, H., Kase, Y., Kaminishi, A., Yachiguchi, K., Kitamura, K., Oshima, Y., Hayakawa, K., Yano, S. and Hattori, A
  • Journal Title: Am. J. Life Sci. Volume: 3 Issue: 3 Pages: 31-38
  • DOI: 10.11648/j.ajls.s.2015030302.16
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] LIPUSに対する骨芽細胞及び破骨細胞の応答:キンギョのウロコを用いたin vitro及びin vivoの解析 (2018)
  • Author(s): 鈴木信雄、半本泰三、田渕圭章、近藤隆、池亀美華、北村敬一郎、関口俊男、小林功、関あずさ、服部淳彦
  • Organizer: 第21回超音波骨折治療研究会
• [Presentation] 魚類の骨代謝に対する黒色素胞刺激ホルモンの影響：in vivo及びin vitroによる解析 (2018)
  • Author(s): 鈴木信雄, 石津偉統, 木谷洋一郎, 五十里雄大, 関口俊男, 北村敬一郎, 遠藤雅人, 服部淳彦, 水澤寛太
  • Organizer: 平成30年度公益社団法人日本水産学会春季大会
• [Presentation] キンギョ高血糖モデルにおける骨代謝および糖化ウロココラーゲンの架橋解析 (2017)
  • Author(s): 北村敬一郎, 鈴木信雄
  • Organizer: 平成29年度日本動物学会 中部支部大会
• [Presentation] Effects of α-melanocyte-stimulating hormone on osteoblasts and osteoclasts in the regenerating scales of goldfish. (2017)
  • Author(s): Ishizu H, Sekiguchi T, Ikari T, Kitamura K, Kitani Y, Endo M, Urata M, Hattori A, Mizusawa K, Takahashi A and Suzuki N
  • Organizer: The 3rd International Symposium to Promote Joint Usage/Research Center 2017
  • Int'l Joint Research
• [Presentation] アロキサン誘発高血糖キンギョモデルにおける骨代謝および糖化ウロココラーゲンの架橋解析. (2016)
  • Author(s): 北村敬一郎, 安藤 忠, 大貝 和裕, 杉谷 加代, 鈴木信雄.
  • Organizer: 第55回日本生体医工学会大会
  • Place of Presentation: 富山国際会議場（富山県富山市）
  • Year and Date: 2016-04-26
• [Presentation] 超小型発汗計による精神性発汗の測定と評価. (2016)
  • Author(s): 大貝和裕, 福岡正和, 北村敬一郎, 内出喜義, 根本鉄.
  • Organizer: 第55回日本生体医工学会大会
  • Place of Presentation: 富山国際会議場（富山県富山市）
  • Year and Date: 2016-04-26
• [Presentation] アロキサン誘発高血糖キンギョにおける骨代謝および骨質の解析. (2015)
  • Author(s): 北村敬一郎, 安藤 忠, 桶作若菜, 服部淳彦, 鈴木信雄
  • Organizer: 日本宇宙生物科学会第29回大会
  • Place of Presentation: 帝京大学 板橋キャンパス（東京都板橋区）
  • Year and Date: 2015-09-26
• [Presentation] 魚類のウロコにおける骨細胞マーカー、sost1の発現と発現細胞の同定. (2015)
  • Author(s): 田崎 裕也, 小倉絵梨奈, 坂井 晴香, 杉谷 加代, 北村敬一郎
  • Organizer: 第40回北陸臨床病理集談会
  • Place of Presentation: 金沢大学保健学類（石川県金沢市）
  • Year and Date: 2015-09-12
• [Presentation] Melatonin effects on stimulated osteoclast activity in the regenerating scales of goldfish under modelled microgravity. (2015)
  • Author(s): Ikegame M, Hattori A, Yamamoto T, Kitamura K, Tabuchi Y, Nakano M, Yano S, Yamamoto T, Suzuki N,
  • Organizer: 13th Congress of the International Society of Bone Morphometry 2015
  • Place of Presentation: Tokyo,Japan
  • Year and Date: 2015-04-27
  • Int'l Joint Research
• [Remarks] 研究者情報
  • URL: http://ridb.kanazawa-u.ac.jp/public/detail.php?id=2619
• [Remarks] 金沢大学 研究者情報
  • URL: http://ridb.kanazawa-u.ac.jp/public/detail.php?id=2619