The role of apoE-rich HDL in lifestyle diseases

• Project/Area Number: 15K01715
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied health science
• Research Institution: Okayama University
• Principal Investigator: USUI SHINICHI 岡山大学, 保健学研究科, 准教授 (50346417)
• Co-Investigator(Kenkyū-buntansha): 廣畑 聡 岡山大学, 保健学研究科, 教授 (90332791)
• Co-Investigator(Renkei-kenkyūsha): SHINOHATA Ryoko 岡山大学, 大学院保健学研究科, 助教 (70335587)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: HDL / アポリポ蛋白E / 肥満 / 生活習慣病モデルマウス / リポ蛋白 / 細胞培養 / 脂肪細胞 / アポリポ蛋白Ｅ / ＨＤＬ

Outline of Final Research Achievements

In this study, we established a quantitative method for serum apoE-rich HDL and showed that apoE-rich HDL increased in obese model mice. In clinical study, multiple regression analysis revealed that triglycerides and adiponectin strongly involved in obesity are significant predictors of apoE-rich HDL-cholesterol levels. However, in the culture experiment of adipocytes, we did not obtain data on which apoE-rich HDL was involved in fat accumulation. In cultured hepatocytes, the production of apoE-rich HDL was increased by the high glucose medium, suggesting that glucose uptake by hepatocytes was significantly involved in the production of apoE-rich HDL. A new research progress is expected in the future in relation to glucose metabolism and the production of E-rich HDL in hepatocytes.

Research Products

• [Journal Article] A High-Fat and High-Cholesterol Diet Induces Cardiac Fibrosis, Vascular Endothelial, and Left Ventricular Diastolic Dysfunction in SHRSP5/Dmcr Rats (2018)
  • Author(s): Watanabe S, Kumazaki S, Kusunoki K, Inoue T, Maeda Y, Usui S, Shinohata R, Ohtsuki T, Hirohata S, Kusachi S, Kitamori K, Mori M, Yamori Y, Oka H.
  • Journal Title: Journal of Atherosclerosis and Thrombosis Volume: 25 Issue: 5 Pages: 439-453
  • DOI: 10.5551/jat.40956
  • NAID: 130006729837
  • ISSN: 1340-3478, 1880-3873
  • Year and Date: 2018-05-01
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Lavender Essential Oil and Its Main Constituents Inhibit the Expression of TNF-α-induced Cell Adhesion Molecules in Endothelial Cells (2017)
  • Author(s): Michinori Aoe, Tomoe Ueno-Iio, Misako Shibakura, Ryoko Shinohata, Shinichi Usui, Yujiro Arao, Satoru Ikeda, Nobuaki Miyahara, Mitsune Tanimoto, and Mikio Kataoka
  • Journal Title: Acta Medica Okayama Volume: 71 Issue: 6 Pages: 493-503
  • DOI: 10.18926/AMO/55586
  • NAID: 120006370713
  • URL: https://ousar.lib.okayama-u.ac.jp/55586
  • Peer Reviewed
• [Journal Article] A rapid and precise method for measuring plasma apoE-rich HDL using polyethylene glycol and cation-exchange chromatography: a pilot study on the clinical significance of apoE-rich HDL measurements. (2017)
  • Author(s): Ikeda T, Shinohata R, Murakami M, Hina K, Kamikawa S, Hirohata S, Kusachi S, Tamura A, Usui S.
  • Journal Title: Clin Chim Acta. Volume: 465 Pages: 112-118
  • DOI: 10.1016/j.cca.2016.12.016
  • Peer Reviewed
• [Presentation] ApoE-rich HDL in pre-heparin plasma may be HDL remnants which remain in the circulation (2017)
  • Author(s): Usui S, Shinohata R, Miyashita K, Hirohata S, Shibakura S, Fukamachi I, Nakajima K
  • Organizer: 69th AACC Annual Scientific Meeting & Clinical Lab Expo
  • Int'l Joint Research
• [Presentation] LDL particle size associates with apoE-containing HDL in patients who undergo coronary computed tomographic angiography. (2016)
  • Author(s): Shinohata R, Ikeda T, Murakami M, Hina K, Kamikawa S, Hirohata S, Kusachi S, Nakajima K, Ikeda S, Usui S.
  • Organizer: 68th AACC Annual Scientific Meeting & Clinical Lab Expo
  • Place of Presentation: Philadelphia(America)
  • Int'l Joint Research
• [Presentation] Hepatic triglyceride lipase is mainly located on apoE-rich HDＬ in post-heparin plasma (2015)
  • Author(s): Usui S, Shinohata R, Nakajima K, Miyashita K, Imamura S, Sato K, Okajima F, Kobayashi J, Shirakawa T, Shimomura Y, Machida T, Sumino H, Murakami M
  • Organizer: 2015 American Association for Clinical Chemistry Annual Meeting
  • Place of Presentation: Ａｔｌａｎｔａ（Ａｍｅｒｉｃａ）
  • Year and Date: 2015-07-29
  • Int'l Joint Research
• [Patent(Industrial Property Rights)] アポＥ含有ＨＤＬの測定方法および測定装置 (2015)
  • Inventor(s): 臼井真一，草地省蔵，臼井綾子
  • Industrial Property Rights Holder: 岡山大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2015-08-10