| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
Molecular pathology of prion diseases is characterized by abnormal prion protein (PrPSc), which involves both structural changes at the molecular level and aggregate formation. Understanding of the interface among PrPSc in the aggregate is a core subject in prion. In general, a bis-functionalized crosslinker is used to analyze. Since the normal and abnormal PrP are identical in the denatured state, it is principally difficult to adapt for access the molecule in the fibril. Thus, methods have been required to mark on a protein molecule in the fibril before denaturation.
I found a method that can solve the lemma by a photo-reaction, furthermore, beneficial sensitizers. As results, I established a crosslinking method that is effective in contaminated systems and selective for PrPSc. Also, the method is compatible with purification and a biochemical method for discrimination of PrPSc from normal one. Finally, an anti-body panel indicated the a specific region of PrPSc confer the interface.
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