• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Possibility of skin care material using Pxt peptides from amphibian (Xenopus tropicalis) skin

Research Project

Project/Area Number 15K01814
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biomolecular chemistry
Research InstitutionWakayama Medical University (2018)
National Institute of Advanced Industrial Science and Technology (2015-2017)

Principal Investigator

Shigeri Yasushi  和歌山県立医科大学, 医学部, 教授 (90357187)

Co-Investigator(Kenkyū-buntansha) 井村 知弘  国立研究開発法人産業技術総合研究所, 材料・化学領域, 研究グループ長 (10371022)
堀江 祐範  国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (30514591)
Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsペプチド / 表面張力 / ミセル / 溶血 / ナノディスク / 両生類 / 抗菌 / 立体構造解析 / 泡 / NMR / nanodisc / antimicrobial peptide / amphibian skin / surface activity / 炎症マーカー / ストレスマーカー
Outline of Final Research Achievements

Twelve novel peptides (Pxt-1-12) were isolated from the skin of Xenopus tropicalis, using topological mass spectrometry analysis. Several ion peaks corresponding to all identified Pxt peptides, were observed with MALDI-MS analysis of X. tropicalis secretory fluids, collected after in vivo stimulation suggesting that Pxt peptides were definitely secretory molecules. The remarkable foaming properties of Pxt-2 and Pxt-5 were observed. We further characterized Pxt-related peptides (Pxt-2, Pxt-5, Pxt-12, reverse Pxt-2, reverse Pxt-5, and reverse Pxt-12). All Pxt-related peptides formed significant amphiphilic alpha-helices. Moreover, Pxt-2, Pxt-5, and reverse Pxt-5 produced the micelle-like assemblies. Pxt-5 possessed the most potent hemolytic activity, while reverse Pxt-5 exhibited the most remarkable cytotoxicity in HaCaT cells. RT-PCR analysis confirmed the gene expressions of Pxt-5 in testis and Pxt-12 in muscle, in addition to skin, while Pxt-2 was only in skin.

Academic Significance and Societal Importance of the Research Achievements

カエル等の両生類は、皮膚を湿った状態に保ち、捕食者や微生物等の外敵から身を守るために、皮膚に多くの粘液腺を有し、抗菌や毒素を含んだ生体分子を積極的に分泌している。そこで独自に開発したトポロジカルマスを駆使した事により、両生類(ネッタイツメガエル)皮膚由来の新たな機能性生体分子(Pxtペプチド)を発見した。カタツムリの粘液由来のカタツムリクリーム、毒蛇由来の蛇毒クリーム(シンエイク, Syn-ake)なるものが最近の流行である事から、Pxtペプチドはこれまで知られていないコスメの添加成分として製品化研究に繋がり、コスメトロジーにおける新分野の開拓に貢献する可能性がある。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (14 results)

All 2019 2018 2017 2016 2015

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (4 results) (of which Invited: 1 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] Mass spectrometry analysis and biological characterization of the venom and venom sac components of the predatory ant Odontomachus monticola.2019

    • Author(s)
      Tani, N., Kazuma, K., Ohtsuka, Y., Shigeri, Y., Masuko, K., Konno, K., Inagaki, H.
    • Journal Title

      Toxins

      Volume: 10 Issue: 1 Pages: 50-50

    • DOI

      10.3390/toxins11010050

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Lipid Nanodisc Formation using Pxt-5 Peptide Isolated from Amphibian (<i>Xenopus tropicalis</i>) Skin, and its Altered Form, Modify-Pxt-52018

    • Author(s)
      Ikeda, Y., Taira, T., Sakai, K., Sakai, H., Shigeri, Y., Imura, T.
    • Journal Title

      Journal of Oleo Science

      Volume: 67 Issue: 8 Pages: 1035-1041

    • DOI

      10.5650/jos.ess18051

    • NAID

      130007429988

    • ISSN
      1345-8957, 1347-3352
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Modification of N-terminal amino acids of fungal benzoate hydroxylase (CYP53A15) for the production of p-hydroxybenzoate and optimization of bioproduction conditions in Escherichia coli.2018

    • Author(s)
      Tamaki, S., Yagi, M., Nishihata, Y., Yamaji, H., Shigeri, Y., Uno, T., and Imaishi, H.
    • Journal Title

      J. Microbiol. Biotech.

      Volume: 28 Issue: 3 Pages: 439-447

    • DOI

      10.4014/jmb.1711.11030

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Isolation of biologically active peptides from the venom of Japanese carpenter bee, Xylocopa appendiculata.2017

    • Author(s)
      Kawakami, H., Goto, S., Murata, K., Matsuda, H., Shigeri, Y., Imura, T., Inagaki, H., and Shinada, T.
    • Journal Title

      Journal of Venomous Animals and Toxins including Tropical Diseases

      Volume: 23 Issue: 1 Pages: 1-11

    • DOI

      10.1186/s40409-017-0119-6

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Novel biologically active peptides isolated from the venom of Xylocopa appendiculata.2017

    • Author(s)
      Kawakami, H., Goto, S., Murata, K., Matsuda, H., Shigeri, Y., Imura, T., Inagaki, H., and Shinada, T.
    • Journal Title

      Journal of Venomous Animals and Toxins including Tropical Diseases

      Volume: 印刷中

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Physicochemical and biological characterizations of Pxt peptides from amphibian (Xenopus tropicalis) skin2016

    • Author(s)
      Shigeri, Y., Horie, M., Yoshida, T., Hagihara, Y., Imura, T., Inagaki, H., Haramoto, Y., Ito, Y., and Asashima, M.
    • Journal Title

      J. Biochem.

      Volume: 159 Issue: 6 Pages: 619

    • DOI

      10.1093/jb/mvw003

    • NAID

      40020869436

    • URL

      https://localhost/en/publications/d12fb343-11a6-4c30-901e-4d225fe38bc4

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Physicochemical and biological characterizations of Pxt peptides from amphibian (Xenopus tropicalis) skin2016

    • Author(s)
      Yasushi Shigeri, Masanori Horie, Tsuyoshi Yoshida, Yoshihisa Hagihara, Tomohiro Imura, Hidetoshi Inagaki, Yoshikazu Haramoto, Yuzuru Ito, and Makoto Asashima
    • Journal Title

      Journal of Biochemistry

      Volume: -

    • NAID

      40020869436

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] アギトアリ毒液成分のペプチドーム及びプロテオーム解析2019

    • Author(s)
      谷直紀、数馬恒平、大塚幸雄、茂里康、増子恵一、紺野勝弘、稲垣英利
    • Organizer
      第三回京都生体質量分析研究会シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] 熱帯ツメガエルの分泌ペプチドを利用した脂質ナノディスクの調製2017

    • Author(s)
      池田ゆり、茂里康、井村知弘
    • Organizer
      日本化学会第97春季年会
    • Place of Presentation
      神奈川県横浜市
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
  • [Presentation] 熱帯ツメガエルの分泌ペプチドを利用した脂質ナノディスクの調製。2017

    • Author(s)
      池田ゆり、茂里康、井村知弘
    • Organizer
      日本化学会第97春季年会(2017)
    • Related Report
      2017 Research-status Report
  • [Presentation] 両生類(ネッタイツメガエル)皮膚由来、機能性ペプチドの網羅的探索2015

    • Author(s)
      茂里康
    • Organizer
      第32回関西地区ペプチドセミナー
    • Place of Presentation
      毎日インテシオ(大阪府)
    • Year and Date
      2015-11-28
    • Related Report
      2015 Research-status Report
    • Invited
  • [Patent(Industrial Property Rights)] カルモジュリン依存性タンパク質リン酸化酵素I由来ポリペプチド、リン酸化剤、および製造方法2016

    • Inventor(s)
      千賀由佳子、秋月一駿、片山将一、茂里康 et al
    • Industrial Property Rights Holder
      千賀由佳子、秋月一駿、片山将一、茂里康 et al
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-095770
    • Filing Date
      2016-05-12
    • Related Report
      2016 Research-status Report
  • [Patent(Industrial Property Rights)] 両生類ネッタイツメガエル皮膚由来機能性ペプチド2015

    • Inventor(s)
      茂里康、萩原義久、渡部邦彦、井村知弘、原本悦和、伊藤弓弦、浅島誠
    • Industrial Property Rights Holder
      茂里康、萩原義久、渡部邦彦、井村知弘、原本悦和、伊藤弓弦、浅島誠
    • Industrial Property Rights Type
      特許
    • Filing Date
      2015
    • Acquisition Date
      2018
    • Related Report
      2018 Annual Research Report
  • [Patent(Industrial Property Rights)] 界面活性ペプチドから短時間で形成する巨大ナノディスク2015

    • Inventor(s)
      井村知弘、茂里康、遠藤明、平敏彰
    • Industrial Property Rights Holder
      井村知弘、茂里康、遠藤明、平敏彰
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2015-163190
    • Filing Date
      2015-08-20
    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi