White box testing of nanomedicines on a microfluidic device

• Project/Area Number: 15K04640
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Nano/Microsystems
• Research Institution: Toyo University
• Principal Investigator: Sasaki Naoki 東洋大学, 理工学部, 准教授 (30462691)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ナノ薬剤 / 血管透過性 / 共培養 / レーザー加工 / マイクロ流体デバイス / 血管

Outline of Final Research Achievements

Nanoparticles have been widely utilized to deliver drugs from blood vessels to tumor tissues. In this study, we developed a microfluidic device to clarify nanoparticle characteristics required for efficient drug delivery. A consumer laser cutter was employed to fabricate the device. Tumor cells and vascular endothelial cells were co-cultured on a membrane-integrated microfluidic device. Permeation of nanoparticles through the endothelial monolayer increased in the presence of tumor cells.

Research Products

• [Journal Article] 民生用レーザー加工機を用いる細胞培養マイクロ流体デバイスの作製 (2018)
  • Author(s): 佐々木直樹, 林知美, 井上奈菜子, 大西真寛
  • Journal Title: 分析化学 Volume: 印刷中
  • NAID: 130007431579
  • Peer Reviewed / Open Access
• [Presentation] 生体にまねぶバイオ分析 (2017)
  • Author(s): 佐々木直樹
  • Organizer: ナノ茶論
  • Invited
• [Presentation] 生体に「まねぶ」バイオ分析 (2017)
  • Author(s): 佐々木直樹
  • Organizer: 東洋大学工業技術研究所講演会
  • Invited
• [Presentation] A membrane-integrated microfluidic device for co-culture analysis (2017)
  • Author(s): Tomomi HAYASHI, Nanako INOUE, Naoki. SASAKI
  • Organizer: RSC Tokyo International Conference 2017
  • Int'l Joint Research
• [Presentation] 多孔膜集積マイクロ流体デバイスによるナノ粒子の透過性分析 (2016)
  • Author(s): 土屋公彰、佐々木直樹
  • Organizer: 日本化学会第96春季年会
  • Place of Presentation: 同志社大学京田辺キャンパス（京都）
  • Year and Date: 2016-03-24
• [Presentation] 腫瘍血管‐間質複合型無細胞マイクロ流体デバイスの開発 (2016)
  • Author(s): 臼井裕也、渡邉学志、佐々木直樹
  • Organizer: 日本化学会第96春季年会
  • Place of Presentation: 同志社大学京田辺キャンパス（京都）
  • Year and Date: 2016-03-24
• [Presentation] 演繹的及び構成的アプローチに基づくマイクロバイオ分析デバイスの開発 (2015)
  • Author(s): 佐々木直樹
  • Organizer: 日本分析化学会第64年会
  • Place of Presentation: 九州大学伊都キャンパス（福岡）（招待講演）
  • Year and Date: 2015-09-09
  • Invited