| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
In microchip electrophoresis, the microanalytical technique for highly efficient preconcentration and separation of biomolecules by the combination of two online sample concentration techniques, large-volume sample stacking with an electroosmotic flow pump (LVSEP) and field-amplified sample injection (FASI). By utilizing the vacuum-drying method for modifying the coating polymers onto the channel surface, it was successfully achieved that analytes injected throughout a microchannel were concentrated by LVSEP, followed by the electrokinetic introduction of concentrated analytes from a sample reservoir by FASI. In the LVSEP-FASI analysis, up to 38,000-fold sensitivity increase was obtained, which was 245-times higher than normal LVSEP. In the analyses of biomolecules, amino acid enantiomers, peptides and amines were enriched with the concentration factors ranging from 120 to 4800.
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