Development of optical detection method for biomolecules by utilizing inorganic nanosheets

• Project/Area Number: 15K05542
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Saga University
• Principal Investigator: SOH Nobuaki 佐賀大学, 農学部, 教授 (90336008)
• Co-Investigator(Renkei-kenkyūsha): KAMADA Kai 長崎大学, 大学院工学研究科, 准教授 (90315284)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 無機ナノシート / 酵素 / 蛍光 / バイオ材料

Outline of Final Research Achievements

In this study, we tried to develop fluorescence detection method for biomolecules by utilizing inorganic nanosheets. First, we developed a fluorescence detection method for hydrogen peroxide using a complex composed of europium-doped titanate nanosheets (Eu-TiOx) and horseradish peroxidase (HRP). Next, we developed a fluorescence detection method for glucose using a complex composed of Eu-TiOx, HRP, and glucose oxidase (GOx). Therefore, Eu-TiOx nanosheets were successfully applied to the development of fluorescence detection method for biomolecules.

Research Products

• [Journal Article] Time-resolved Fluorescent Detection for Glucose Using a Complex of Luminescent Layered Titanates and Enzymes (2017)
  • Author(s): SAKAGUCHI Yuki、MINAMIKAWA Tomoka、YAMAMURO Mayuko、TSUJITA Tadayuki、UEDA Toshihisa、KAMADA Kai、SOH Nobuaki
  • Journal Title: Analytical Sciences Volume: 33 Issue: 9 Pages: 989-991
  • DOI: 10.2116/analsci.33.989
  • NAID: 130006071189
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed / Open Access
• [Journal Article] Visible-light-induced activity control of peroxidase bound to Fe-doped titanate nanosheets with nanometric lateral dimensions (2015)
  • Author(s): Kai Kamada, Daiki Ito, Nobuaki Soh
  • Journal Title: Bioconjugate Chem. Volume: 26 Issue: 10 Pages: 2161-2166
  • DOI: 10.1021/acs.bioconjchem.5b00464
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] ユウロピウムをドープした無機ナノシートと酵素の複合体による燐光モードでのグルコース検出に関する検討 (2017)
  • Author(s): 坂口優紀・山室麻由子・上田敏久・鎌田 海・宗 伸明
  • Organizer: 第54回化学関連支部合同九州大会
• [Presentation] 無機ナノシート/酵素/磁気ビーズ複合体によるコリン検出系の開発と磁気ビーズの粒径が及ぼす影響の検討 (2017)
  • Author(s): 山下舞美・上田敏久・鎌田 海・宗 伸明
  • Organizer: 第54回化学関連支部合同九州大会
• [Presentation] Fluorescent detection of biomolecules based on organic compounds and nanobiohybrids (2017)
  • Author(s): Nobuaki Soh
  • Organizer: Joint-Seminar of Yonsei University and National Universities in Kyushu
  • Invited
• [Presentation] Development of bioanalytical method based on titanate nanosheets (2016)
  • Author(s): Nobuaki Soh
  • Organizer: Rare Earths 2016 in Sapporo
  • Place of Presentation: Hokkaido, Japan
  • Year and Date: 2016-06-06
  • Int'l Joint Research / Invited
• [Presentation] 蛍光性無機ナノシート/酵素複合体を用いた過酸化水素並びにグルコース検出系の開発 (2016)
  • Author(s): 南川朋花・坂口優紀・上田敏久・鎌田 海・宗 伸明
  • Organizer: 第53回化学関連支部合同九州大会
  • Place of Presentation: 福岡県北九州市
• [Presentation] 無機ナノシート/酵素/磁気ビーズ複合体によるコリン検出系の開発 (2016)
  • Author(s): 牟田典恵・山下舞美・上田敏久・鎌田 海・宗 伸明
  • Organizer: 第53回化学関連支部合同九州大会
  • Place of Presentation: 福岡県北九州市
• [Presentation] 無機ナノシートに二種の酵素と磁気ビーズを複合化したハイブリッド体の開発と酵素の繰り返し利用 (2015)
  • Author(s): 南川朋花・牟田典恵・藤島 稜・上田敏久・宗 伸明・鎌田 海
  • Organizer: 日本分析化学会第64年会
  • Place of Presentation: 福岡県福岡市
  • Year and Date: 2015-09-09
• [Book] Rational Design of Enzyme-Nanomaterials, Methods in Enzymology, Vol. 571 (Challa Vijaya Kumar, Ed.) (2016)
  • Author(s): Kai Kamada, Akane Yamada, Misato Kamiuchi, Motoko Tokunaga, Daiki Ito, Nobuaki Soh
  • Total Pages: 264
  • Publisher: Academic Press (UK)