Development of inspection system for diagnosis of infectious diseases using a compact disk-type microfluidic device

• Project/Area Number: 15K05544
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Tokyo Metropolitan University
• Principal Investigator: NAKAJIMA Hizuru 首都大学東京, 都市環境科学研究科, 准教授 (10432858)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: CD型マイクロチップ / マイクロ化学分析システム / 蛍光 / 酵素免疫測定法 / オンサイト測定 / POCT / 感染症 / コンパクトディスク / CD / マイクロチップ

Outline of Final Research Achievements

A novel compact disk (CD)-type fluorescence detection system capable of real-time measurement was developed for rapid diagnosis of infectious diseases. This fluorescence detection system can automatically perform analytical operations including sample introduction, separation and detection on a CD-type microfluidic device by rotating a light source (LED) and a photodetector (photodiode) with the CD-type microfluidic device. A portable fluorescence detection system that could run on a battery was also developed for point of care testing (POCT). This portable fluorescence detection system was successfully used in an antibody test on infectious diseases.

Research Products

• [Journal Article] Elaborately programmed nanowires fabricated using a tapered push-pull nozzle system (2018)
  • Author(s): Yong Zhang, Sifeng Mao, Yuma Suzuki, Yumi Tanaka, Masato Kawaguchi, Weifei Zhang, Hulie Zeng, Hizuru Nakajima, Ming Yang, Katsumi Uchiyama
  • Journal Title: Chem. Comm. Volume: 54 Issue: 7 Pages: 719-722
  • DOI: 10.1039/c7cc07873k
  • Peer Reviewed
• [Journal Article] Reversibly electro-controllable polymer brush for electro-switchable friction (2017)
  • Author(s): Hulie Zeng, Sifeng Mao, Yong Zhang, Hizuru Nakajima, Katsumi Uchiyama
  • Journal Title: J. Mater. Chem. C Volume: 5 Issue: 24 Pages: 5877-5881
  • DOI: 10.1039/c7tc01624g
  • Peer Reviewed
• [Journal Article] Stably electro-switchable poly-allyloxy hydroxypropyl sulfonate branched brush towards reversible capture and release of proteins and cells (2017)
  • Author(s): Hulie Zeng, Yong Zhang, Hizuru Nakajima, Katsumi Uchiyama
  • Journal Title: Sensor. Actuat. B Volume: 251 Pages: 334-338
  • DOI: 10.1016/j.snb.2017.05.032
  • Peer Reviewed
• [Journal Article] Inkjet printing based assembly of thermoresponsive core-shell polymer microcapsules for controlled drug release (2016)
  • Author(s): Jianmin Yang, Daisuke Katagiri, Sifeng Mao, Hulie Zeng, Hizuru Nakajima, Shungo Kato, Katsumi Uchiyama
  • Journal Title: Journal of Materials Chemistry B Volume: 4 Issue: 23 Pages: 4156-4163
  • DOI: 10.1039/c6tb00424e
  • Peer Reviewed
• [Journal Article] Development of a Portable Surface Plasmon Resonance Sensor with Multi-Sensing Points Based on the Linear CCD Sensor (2016)
  • Author(s): Jie ZHAN, Koji FURUI, Hizuru NAKAJIMA, Noriaki KANEKI, Ryoichi ISHIMATSU, Koji NAKANO, Toshihiko IMATO, Akihide HEMMI
  • Journal Title: Analytical Sciences Volume: 32 Issue: 6 Pages: 673-679
  • DOI: 10.2116/analsci.32.673
  • NAID: 130005156496
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed / Open Access
• [Journal Article] Droplet Enhanced Fluorescence for Ultrasensitive Detection Using Inkjet (2016)
  • Author(s): Hulie Zeng, Daisuke Katagiri, Taisuke Ogino, Hizuru Nakajima, Shungo Kato, Katsumi Uchiyama
  • Journal Title: Analytical Chemistry Volume: 88 Issue: 12 Pages: 6135-6139
  • DOI: 10.1021/acs.analchem.6b01566
  • Peer Reviewed
• [Journal Article] Microchemical Pen: An Open Microreactor for Region-Selective Surface Modification (2016)
  • Author(s): S. Mao, C. Sato, Y. Suzuki, J. Yang, H. Zeng, H. Nakajima, M. Yang, J-M. Lin, K. Uhciyama
  • Journal Title: ChemPhysChem Volume: 17 Issue: 20 Pages: 3155-3159
  • DOI: 10.1002/cphc.201600857
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The use of an inkjet injection technique in immunoassays by quantitative on-line electrophoretically mediated microanalysis (2016)
  • Author(s): Weifei Zhang, Sifeng Mao, Jianmin Yang, Hulie Zeng, Hizuru Nakajima, Shungo Kato, Katsumi Uchiyama
  • Journal Title: Journal of Chromatography A Volume: 1477 Pages: 127-131
  • DOI: 10.1016/j.chroma.2016.11.041
  • Peer Reviewed
• [Journal Article] Investigation of simultaneous immunoassay by a two-dimensional surface plasmon resonance sensor using multiplied beam splitting optics (2016)
  • Author(s): Kazuhiro Morioka, Hizuru Nakajima, Akihide Hemmi, Shinpei Iida, Fumi Kitamura, Hulie Zeng, Shungo Kato, Katsumi Uchiyama
  • Journal Title: Bunsekikagaku Volume: 65 Pages: 79-85
  • NAID: 130005131107
  • Peer Reviewed
• [Journal Article] Investigation of monodisperse droplet generation in liquids by inkjet (2015)
  • Author(s): Hulie Zeng, Jianmin Yang, Daisuke Katagiri, Ying Rang, Shuhua Xue, Hizuru Nakajima, Katsumi Uchiyama
  • Journal Title: Sensors and Actuators B Volume: 220 Pages: 958-961
  • DOI: 10.1016/j.snb.2015.06.027
  • Peer Reviewed
• [Journal Article] Quantitative on-line concentration for capillary electrophoresis with inkjet sample introduction technique (2015)
  • Author(s): Ying Rang, Hulie Zeng, Hizuru Nakajima, Shungo Kato, Katsumi Uchiyama
  • Journal Title: Journal of Separation Sciences Volume: 38 Issue: 15 Pages: 2722-2728
  • DOI: 10.1002/jssc.201500201
  • Peer Reviewed
• [Presentation] Development of surface plasmon resonance sensor using an optical fiber prepared by electroless displacement gold plating method (2018)
  • Author(s): Miyu Nakajima, Atsushi Shoji, Kenji Morita, Kazuhiro Morioka, Akio Yanagida, Akihide Hemmi, Hulie Zeng, Shungo Kato, Katsumi Uchiyama, Hizuru Nakajima
  • Organizer: PITTCON2018, Orlando, Florida , USA
  • Int'l Joint Research
• [Presentation] Development of Portable Fluorescence Detection System Using an Organic Photodiode Array Detector (2017)
  • Author(s): Kazuhiro Morioka, Hizuru Nakajima, Akihide Hemmi, Hulie Zeng, Shungo Kato, Katsumi Uchiyama
  • Organizer: PITTCON2017
  • Place of Presentation: Chicago (USA)
  • Year and Date: 2017-03-05
  • Int'l Joint Research
• [Presentation] μTASのための温度応答性マイクロバルブの開発 (2017)
  • Author(s): 田中 佑実, 曽 湖烈, 加藤 俊吾, 中嶋 秀, 内山 一美
  • Organizer: 日本分析化学会第66年会, 東京理科大学葛飾キャンパス
• [Presentation] ピペットチップ内壁を反応場とするオンサイト測定用蛍光検出システムの開発 (2017)
  • Author(s): 長嶋 萌子, 森岡 和大, 中嶋 秀, 辺見 彰秀, 曾 湖烈, 加藤 俊吾, 内山 一美
  • Organizer: 日本分析化学会第66年会, 東京理科大学葛飾キャンパス
• [Presentation] 有機フォトダイオードを用いる蛍光マイクロプレートリーダーの開発 (2017)
  • Author(s): 森岡 和大, 長嶋 萌子, 中嶋 秀, 辺見 彰秀, 曾 湖烈, 加藤 俊吾, 東海林 敦, 柳田 顕郎, 内山 一美
  • Organizer: 日本分析化学会第77回分析化学討論会, 龍谷大学深草学舎
• [Presentation] 無電解金めっき技術に基づく光ファイバー表面プラズモン共鳴センサーのイムノアッセイへの応用 (2016)
  • Author(s): 中島 美優, 植野 琴美, 森岡 和大, 東海林 敦, 辺見 彰秀, 柳田 顕郎, 中嶋 秀, 内山 一美
  • Organizer: 日本分析化学会第65年会
  • Place of Presentation: 北海道大学（北海道札幌市）
  • Year and Date: 2016-09-14
• [Presentation] オンサイト分析を指向した携帯型化学発光測定システムの開発 (2016)
  • Author(s): 森岡 和大, 長嶋 萌子, 中嶋 秀, 辺見 彰秀, 曽 湖烈, 加藤 俊吾, 内山 一美
  • Organizer: 日本分析化学会第65年会
  • Place of Presentation: 北海道大学（北海道札幌市）
  • Year and Date: 2016-09-14
• [Presentation] DVD-Rを用いる表面プラズモン共鳴センサの開発 (2016)
  • Author(s): 浅田 麻帆, 森岡 和大, 中嶋 秀, 辺見 彰秀, 曽 湖烈, 加藤 俊吾, 内山 一美
  • Organizer: 日本分析化学会第65年会
  • Place of Presentation: 北海道大学（北海道札幌市）
  • Year and Date: 2016-09-14
• [Presentation] 無電解メッキの real-time 膜厚変化観察法と光ファイバー表面プラズモン共鳴センサーの作製への応用 (2016)
  • Author(s): 東海林 敦, 佐坂 徳成, 内田 大貴, 中村 邦彦, 森田 健司, 中島 美優, 辺見 彰秀, 中嶋 秀, 内山 一美, 柳田 顕郎
  • Organizer: 第76回分析化学討論会
  • Place of Presentation: 岐阜薬科大学・岐阜大学（岐阜県岐阜市）
  • Year and Date: 2016-05-28
• [Presentation] 有機ELと有機フォトダイオードを用いる蛍光検出システムの開発 (2016)
  • Author(s): 森岡 和大, 中嶋 秀, 辺見 彰秀, 曽 湖烈, 加藤 俊吾, 内山 一美
  • Organizer: 第76回分析化学討論会
  • Place of Presentation: 岐阜薬科大学・岐阜大学（岐阜県岐阜市）
  • Year and Date: 2016-05-28
• [Presentation] 無電解金めっき技術に基づく光ファイバー表面プラズモン共鳴センサーの構築 (2016)
  • Author(s): 中島 美優, 東海林 敦, 辺見 彰秀, 柳田 顕郎, 中嶋 秀, 内山 一美
  • Organizer: 第76回分析化学討論会
  • Place of Presentation: 岐阜薬科大学・岐阜大学（岐阜県岐阜市）
  • Year and Date: 2016-05-28
• [Presentation] コンパクトディスク型マイクロチップを用いる蛍光検出システムの開発と感染症検査への応用 (2016)
  • Author(s): 髙橋 遼, 森岡 和大, 中嶋 秀, 辺見 彰秀, 曽 湖烈,加藤 俊吾, 内山 一美
  • Organizer: 第76回分析化学討論会
  • Place of Presentation: 岐阜薬科大学・岐阜大学（岐阜県岐阜市）
  • Year and Date: 2016-05-28
• [Presentation] Development of Portable ELISA System for Infectious Disease Diagnosis (2016)
  • Author(s): Kazuhiro Morioka, Harpal Singh, Hizuru Nakajima, Akihide Hemmi, Masayuki Shimojima,, Le Van An, Sazaly AbuBakar , Hulie Zeng, Shungo Kato, Masami Sugamata, Ming Yang, Katsumi Uchiyama
  • Organizer: Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy (PITTCON2016)
  • Place of Presentation: Georgia World Congress Center (USA)
  • Year and Date: 2016-03-06
  • Int'l Joint Research
• [Presentation] Development of Portable Microplate Reader and Its Application to Viral Antibody Test (2015)
  • Author(s): Hizuru Nakajima, Kazuhiro Morioka, Akihide Hemmi, Katsumi Uchiyama
  • Organizer: The 35th Symposium on Capillary electrophoresis (SCE2015)
  • Place of Presentation: Okayama University (Okayama)
  • Year and Date: 2015-11-04
  • Invited
• [Presentation] Development of portable ELISA system for infectious disease diagnosis (2015)
  • Author(s): Kazuhiro Morioka, Harpal Singh, Hizuru Nakajima, Akihide Hemmi, Masayuki Shimojima,, Le Van An, Sazaly AbuBakar , Hulie Zeng, Shungo Kato, Masami Sugamata, Ming Yang, Katsumi Uchiyama
  • Organizer: The 16th Beijing Conference and Exhibition on Instrumental Analysis (BCEIA 2015)
  • Place of Presentation: China National Convention Center (China)
  • Year and Date: 2015-10-27
  • Int'l Joint Research
• [Presentation] Development of surface plasmon resonance sensor using DVD-R (2015)
  • Author(s): Maho Asada, Kazuhiro Morioka, Hizuru Nakajima, Akihide Hemmi, Hulie Zeng, Shungo Kato, Katsumi Uchiyama
  • Organizer: The 16th Beijing Conference and Exhibition on Instrumental Analysis (BCEIA 2015)
  • Place of Presentation: China National Convention Center (China)
  • Year and Date: 2015-10-27
  • Int'l Joint Research
• [Presentation] Development of palm-sized ELISA system for the rapid and on-site diagnosis of infection disease (2015)
  • Author(s): Kazuhiro Morioka, Harpal Singh, Hizuru Nakajima, Akihide Hemmi, Masami Sugamata, Le Van An, Sazaly AbuBakar, Hulie Zeng, Shungo Kato1, Ming Yang, Katsumi Uchiyama
  • Organizer: The 64th annual meeting of the Japan Society for Analytical Chemistry, 2nd Asian Symposium for Analytical Sciences
  • Place of Presentation: Kyushu University (Fukuoka)
  • Year and Date: 2015-09-09
  • Int'l Joint Research
• [Remarks] 首都大学東京教員紹介
  • URL: https://www.tmu.ac.jp/stafflist/data/na/653.html
• [Remarks] 首都大学東京教員紹介
  • URL: http://www.tmu.ac.jp/stafflist/data/na/653.html