Glycosylation using plant enzymes to produce anti-histamine agent

• Project/Area Number: 15K05576
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: Okayama University of Science
• Principal Investigator: Hamada Hiroki 岡山理科大学, 理学部, 教授 (10164914)
• Co-Investigator(Kenkyū-buntansha): 下田 恵 大分大学, 医学部, 准教授 (40284153)
• Research Collaborator: UESUGI Daisuke
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: 酵素変換 / 糖転移酵素 / ヒスタミン遊離抑制 / 酵素化学

Outline of Final Research Achievements

Cultured plant cells and their enzymes are useful for studying the biosynthesis of secondary metabolites, such as flavors, pigments, and agrochemicals from view of green chemistry. To date the biotransformation of various organic compounds has been investigated for the biotechnological application of plant cultured cells and enzymes. The reactions involved in the biotransformation of organic compounds by cultured plant cells and enzymes include methylation and glycosylation. Particularly, glycosylation attracts pharmaceutical attention because many glycosides show anti-inflammatory and anti-histamine activities. In this study, capsaicin and stilbenoids were glycosylated and methylated by cultured plant cells and plant enzymes.

Research Products

• [Journal Article] Synthesis, oxygen radical absorbance capacity, and tyrosinase inhibitory activity of glycosides of resveratrol, pterostilbene, and pinostilbene. (2017)
  • Author(s): Daisuke Uesugi, Hiroki Hamada, Kei Shimoda, Naoji Kubota, Shin-ichi Ozaki, and Naoki Nagatani
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 81 Pages: 226-230
  • Peer Reviewed
• [Journal Article] Synthesis, oxygen radical absorbance capacity, and tyrosinase inhibitory activity of glycosides of resveratrol, pterostilbene, and pinostilbene. (2017)
  • Author(s): Daisuke Uesugi, Hiroki Hamada, Kei Shimoda, Naoji Kubota, Shin-ichi Ozaki, Naoki Nagatani
  • Journal Title: Biosci., Biotechnol., Biochem. Volume: 81 Pages: 226-230
  • Peer Reviewed
• [Journal Article] Synthesis and pharmacological evaluation of glycosides of resveratrol, pterostilbene, and piceatannol. (2015)
  • Author(s): K. Shimoda, N. Kubota, D. Uesugi, H. Hamada, M. Tanigawa, H. Hamada
  • Journal Title: Annals of the New York Academy of Sciences Volume: 1348 Pages: 141-149
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] ヨウシュヤマゴボウ培養細胞由来糖転移酵素を用いたスチルベン誘導体の配糖化 (2017)
  • Author(s): 濱田博喜,藤高侑也,荒木美奈実,上杉大介,下田恵,小崎紳一,井上豪
  • Organizer: 第80回 日本植物細胞分子生物学会
• [Presentation] 植物培養細胞を用いたN-グリコシル化 (2016)
  • Author(s): 荒木美奈実,川村章吾,中山騎維,小野翼,真鍋光一,下田恵,小崎紳一,濱田博喜
  • Organizer: 第34回日本植物細胞分子生物学会
  • Place of Presentation: 信州大学繊維学部 長野県上田市
  • Year and Date: 2016-09-01
• [Presentation] Synthesis and evaluation of glycosides of trans-resveratrol,pterostillbene,and piceatannol (2015)
  • Author(s): Shota Okada, Daisuke Uesugi, Noriyuki Nakayama, Shota Doi, Shogo Kawamura, Kei Shimoda, Shinichi Ozaki, Hiroki Hamada
  • Organizer: ISPSA2015 TOKUSHIMA
  • Place of Presentation: 徳島
  • Year and Date: 2015-08-28
  • Int'l Joint Research