Evaluation of the enhancement of osteogenesis by Zn-releasing diamond-like carbon film
| Project/Area Number |
15K06470
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Composite materials/Surface and interface engineering
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| Research Institution | Tokyo Denki University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
馬目 佳信 東京慈恵会医科大学, 医学部, 教授 (30219539)
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| Research Collaborator |
OHGOE Yasuharu
FUJIOKA Kouki
HIRATSUKA Masanori
TUBOI Hitomi
NAKAMORI Hideki
MASUDA Hideki
HONDA Hiroshi
MABUCHI Yasufumi
KATOUNO Junichi
KIDERA Shunta
SAITOU Kazuhiro
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | DLCコーティング / ドーピング / 骨芽細胞 / 生体親和性 / Zn徐放 / 元素ドーピング |
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| Outline of Final Research Achievements |
Diamond-like carbon (DLC) has a wide range of commercial applications and some types are known to be biologically stable. Zinc (Zn) has received attention because it enhances the production of alkaline phosphatase(ALP), which promotes bone calcification. Therefore, we manufactured a DLC that contains Zn (Zn-DLC) and can supply Zn to biological systems, as a potential biomaterial to enhance osteogenesis. We evaluated film crystallinity using Raman spectroscopy and measured the amount of released Zn using inductively coupled plasma mass spectrometry (ICP-MS). In addition, we evaluated the effects of Zn-DLC on osteogenesis in vitro. Osteoblasts cultured on Zn-DLC tended to show a greater area of calcification than those cultured on DLC, although no significant differences in ALP activity were observed. These results suggested the possibility that osteogenesis could be enhanced by the Zn that is released from Zn-DLC.
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Report
(4 results)
Research Products
(20 results)
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[Presentation] Enhancement of osteogenic on Zn releace with Zn doped DLC film coating2016
Author(s)
Junichi Katono, Yasuharu Ohgoe, Koki Fujioka, Masanori Hiratsuka, Hitomi Tsuboi, Hideki Nakamori, Hideki Masuda, Hiroshi Honda, Yoshinobu Manome, Kenji Hirakuri
Organizer
The 5th Asia-Pacific International Congress on Engineering & Natural Sciences
Place of Presentation
Loisir Hotel, Naha (Okinawa, Japan)
Year and Date
2016-08-02
Related Report
Int'l Joint Research
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[Presentation] Structural Investigation of Zn Doped Diamond-Like Carbon Films2016
Author(s)
Junichi Katouno, Yasufumi Mabuchi, Yasuharu Ohgoe, Keisuke Sato, Kouki Fujioka, Yoshinobu Manome, Masanori Hiratsuka, Hideki Nakamori, Hideki Masuda, Hiroshi Honda, Kenji Hirakuri
Organizer
The 5th Asia-Pacific International Congress on Engineering & Natural Sciences
Place of Presentation
Loisir Hotel, Naha (Okinawa, Japan)
Year and Date
2016-08-02
Related Report
Int'l Joint Research
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[Presentation] Zn徐放型DLC膜の骨形成促進効果の評価2015
Author(s)
馬渕 康史, 上遠野惇市, 大越 康晴, 藤岡 宏樹, 佐藤 慶介, 平塚 傑工, 坪井 仁美, 中森 秀樹, 益田 秀樹, 本田 宏志, 馬目 佳信, 平栗 健二
Organizer
第29回ダイヤモンドシンポジウム
Place of Presentation
東京理科大学
Year and Date
2015-11-17
Related Report
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[Presentation] 亜鉛含有DLC膜における亜鉛徐放量の制御2015
Author(s)
上遠野惇市,馬渕康史,藤岡宏樹,大越康晴,佐藤慶介,平塚傑工,坪井仁美,中森秀樹,益田秀樹,本田宏志,馬目佳信,平栗健二
Organizer
第76回応用物理学会秋季学術講演会
Place of Presentation
名古屋国際会議場
Year and Date
2015-09-13
Related Report
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[Presentation] Cytotoxicity of Zn-doped DLC for osteoblasts2015
Author(s)
Yasufumi Mabuchi, Yasuharu Ohgoe, Kouki Fujioka, Keisuke Sato, Masanori Hiratsuka, Hitomi Tsuboi, Hideki Nakamori, Hideki Masuda, Hiroshi Honda, Yoshinobu Manome, Kenji Hirakuri
Organizer
International Conference On Diamond and Carbon Materials
Place of Presentation
Homburg, Germany
Year and Date
2015-09-06
Related Report
Int'l Joint Research
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