Mechanisms of glial development

• Project/Area Number: 15K06719
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Keio University
• Principal Investigator: Shimazaki Takuya 慶應義塾大学, 医学部(信濃町), 准教授 (00324749)
• Research Collaborator: TSUYAMA Jun
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 神経幹細胞 / グリア / 分化 / マーカー

Outline of Final Research Achievements

We attempted to develop a system to visualize glial-restricted progenitors (GRPs) and/or transition state of neural stem cell (NSC) differentiation into glia. We identified Kcnj16 which is an inward-rectifier type potassium channel as a marker for GRPs or glial differentiation of NSCs via expression profiling to identify genes which respond to the temporal changes of gliogenic potency of NSCs during development and in situ hybridization analysis of expression pattern of candidate genes during central nervous system development. Finally, it has been suggested that a reporter system which can recapitulate the expression pattern of Kcnj16 can be applicable to visualize GRPs or glial differentiation of NSCs.

Research Products

• [Journal Article] Heterochronic microRNAs in temporal specification of neural stem cells: application toward rejuvenation (2016)
  • Author(s): Shimazaki T*, Okano H*
  • Journal Title: npj Aging and Mechanisms of Disease Volume: 2 Issue: 1 Pages: 15014-15014
  • DOI: 10.1038/npjamd.2015.14
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Vertebrate Neural Stem Cells: Development, Plasticity, and Regeneration. (2016)
  • Author(s): Shimazaki T*
  • Journal Title: The Keio Journal of Medicine Volume: 65 Issue: 1 Pages: 1-15
  • DOI: 10.2302/kjm.2015-0005-ir
  • NAID: 130005138573
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] MicroRNA-153 regulates the acquisition of gliogenic competence by neural stem cells. (2015)
  • Author(s): Tsuyama J, Bunt J, Richards LJ, Iwanari H, Mochizuki Y, Hamakubo T, Shimazaki T*, Okano H*
  • Journal Title: Stem Cell Reports Volume: 5 Issue: 3 Pages: 365-377
  • DOI: 10.1016/j.stemcr.2015.06.006
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 神経幹/前駆細胞においてニューロン新生からグリア新生への切り替えを制御する調節因子 (2015)
  • Author(s): 津山 淳，島崎 琢也，岡野 栄之
  • Organizer: 第３８回日本神経科学大会
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2015-07-29
• [Presentation] 神経幹細胞のグリア分化能を制御する発生時期特異的な因子の同定 (2015)
  • Author(s): 津山 淳，島崎 琢也，岡野 栄之
  • Organizer: 第36回日本炎症・再生医学会
  • Place of Presentation: 虎ノ門ヒルズフォーラム（東京都港区）
  • Year and Date: 2015-07-21