| Project/Area Number |
15K06858
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor diagnostics
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| Research Institution | Juntendo University |
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Principal Investigator |
Kazuno Saiko 順天堂大学, 医学(系)研究科(研究院), 助教 (00338344)
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| Co-Investigator(Kenkyū-buntansha) |
上野 隆 順天堂大学, 医学(系)研究科(研究院), 客員教授 (10053373)
藤村 務 東北医科薬科大学, 薬学部, 教授 (70245778)
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 前立腺がん / プロテオーム解析 / 糖鎖 / バイオマーカー / レクチン / 糖タンパク質 / マルチプレックス |
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| Outline of Final Research Achievements |
The purpose of our study is to develop a low invasive, definitive diagnostic method to overcome frequent errors of PSA, a widely used diagnostic marker for prostate cancer. Using lectin array, we first characterized glycosylation status of serum glycoproteins and found that 0-glycans were more enriched in patients’ sera. O-glycan-bearing serum glycoproteins were comprehensively collected using O-glycan-specific lectins and subsequently analyzed by the quantitative proteomic analysis. It was found that clusterin in malignant sera was more O-glycosylated than that in benign patients or control subjects. We further developed a new method to quantify O-glycosylated clusterin in the serum: serum clusterin that had been adsorbed on anti-clusterin IgG-immobilized fluorescent beads was probed with another fluorescent O-glycan-specific lectin beads using a Luminex 200. The method is more reliable to distinguish prostate cancer patients from benign prostate disease and healthy person.
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| Academic Significance and Societal Importance of the Research Achievements |
前立腺がんマーカーであるPSA値はがん特異性が低い問題点がありグレーゾーンといわれる範囲に悪性と良性患者が混在し、生検に依存しないPSA値を補完する低侵襲ながん診断法の確立が望まれる。本研究は抗体結合ビーズ‐レクチン検出法によるPSA値診断の問題克服を目指した。clusterinをターゲットとして開発した蛍光ビーズ結合抗体－レクチン検出法はROC分析から特にPSA値10ng/ml以下のグレーゾーン患者においてPSA値より優れた結果が得られた。本法は有意に前立腺がん患者を識別できることを確認し、PSA値による診断の問題点を補完する可能性が示唆された。
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