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Establishing reintroduction and phylogenetic conservation measures based on the genetic diversity evaluation of the endangered aquatic insect

Research Project

Project/Area Number 15K06940
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Conservation of biological resources
Research InstitutionNatural History Museum and Institute, Chiba

Principal Investigator

KURANISHI Ryoichi  千葉県立中央博物館, その他部局等, 研究員(移行) (10250143)

Co-Investigator(Kenkyū-buntansha) 栗田 隆気  千葉県立中央博物館, その他部局等, 研究員(移行) (00738841)
Co-Investigator(Renkei-kenkyūsha) NISHIHARA Syogo  東京大学, 大学院農学生命科学研究科(農学部), 特任研究員 (90569625)
Research Collaborator SHINZATO Naoya  琉球大学, 熱帯生物圏研究センター, 准教授
HORIGUCHI Tomohiro  千葉大学, 園芸学部, 大学院生
ONO Tomoki  千葉県環境生活部自然保護課生物多様性センター, 主幹
SADO Tetsuya  千葉県立中央博物館, 共同研究員
Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords絶滅危惧種 / 保全生物学 / マイクロサテライト / 遺伝的多様性 / 再導入 / 系統保存 / マイクロサテライトマーカー / 保全遺伝学 / 核DNA
Outline of Final Research Achievements

For the conservation of an endangered insect species, we examined the method of phylogenetic preservation and reintroduction based on genetic information. For obtaining genetic information, we developed microsatellite markers using giga sequencer platforms. We examined the genetic diversity, continuity, and uniqueness of regional populations using these microsatellite markers. On the basis of these results, a plan was implemented to reintroduce phylogenetically conserved populations in the field.

Academic Significance and Societal Importance of the Research Achievements

日本の水生生物は生息環境の悪化や消失によって急速に集団サイズを減少させつつある。遺伝的多様性情報の欠落、集団遺伝学的データの活用法が確立されていないことが保全実務への大きな障壁となっていた。
本研究では絶滅危惧水生昆虫について、遺伝的多様性の維持を柱の一つに組み込んだ保全策を検討し、保全方法論の標準化を試みることを目的とした。特に絶滅危惧種シャープゲンゴロウモドキを対象に核DNAの遺伝的多様性に基づく地域個体群の健全性の評価、地域個体群間の遺伝的接続性と保全単位の解明を行った。これらの結果を統合し、地域個体群としての遺伝的独自性が失われない系統保存計画、飼育系統の野外への再導入計画を策定した。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (40 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (8 results) Journal Article (12 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 10 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (19 results) (of which Int'l Joint Research: 5 results,  Invited: 1 results) Book (1 results)

  • [Int'l Joint Research] Biodiversity and climate Research/Berlin Museum Naturkunde(ドイツ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Royal Ontrio Museum(カナダ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Smithonian Institution(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Dayeh University(その他の国・地域 台湾)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Biodiversity and Climate Research C.(Germany)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] Dayeh University(Taiwan)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] Royal Ontario Museum(Canada)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] Smithsonian Institution(米国)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Discovery of Eubasilissa signata Wiggins,1998 (Trichoptera, Phryganeidae) from Taiwan2019

    • Author(s)
      Kuranishi, R.B ・HSU Li-peng
    • Journal Title

      Zoosymposia

      Volume: 印刷中

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 人囮法によるヒトスジシマカ成虫の飛来時間2019

    • Author(s)
      木村悟朗・倉西良一
    • Journal Title

      千葉県立中央博物館自然誌研究報告

      Volume: 14 Pages: 77-80

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Phylogeographic analyses of the Stenopsyche caddisflies (Trichoptera: Stenopsychidae) of the Asian Region2018

    • Author(s)
      Saito Rie、Kato Shinya、Kuranishi Ryoichi B.、Nozaki Takao、Fujino Takeshi、Tojo Koji
    • Journal Title

      Freshwater Science

      Volume: 37 Issue: 3 Pages: 562-572

    • DOI

      10.1086/699364

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 伊豆諸島八丈島における国内外来種ニホンイタチの糞による食性分析2018

    • Author(s)
      土井 学・倉西 良一
    • Journal Title

      日本生物地理学会会報

      Volume: 72 Pages: 113-125

    • NAID

      40021483860

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Phylogeographic analyses of the Stenopsyche caddisflies (Trichoptera:Stenopsychidae) of the Asian Region2018

    • Author(s)
      Saito, R., Kato, S., Kuranishi, R. B., Nozaki, T., Fujino, T., K. Tojo
    • Journal Title

      Freshwater Science

      Volume: 印刷中

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] 伊豆諸島利島における国内外来種ニホンイタチの在来種への影響2017

    • Author(s)
      土井学・西海功・倉西良一
    • Journal Title

      日本生物地理学会会報

      Volume: 71 Pages: 53-62

    • NAID

      40021069563

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] The First Record of Ephemeroptera on Minami-daito-jima Island: Information on the Cloeon sp. Specimens Collected and Their Genetic Relationships2017

    • Author(s)
      Koji YANO, Ryoichi R. KURANISHI, Masaaki KIMURA, Masaru AOYAGI and Koji TOJO
    • Journal Title

      NEW ENTOMOLOGIST

      Volume: 印刷中

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] 東京大学千葉演習林(千葉県南部清澄山系)の昆虫相2017

    • Author(s)
      斉藤明子・尾崎煙雄・宮野伸也・鈴木勝・斉藤修・村川功雄・倉西良一
    • Journal Title

      千葉中央博自然誌研究報告特別号

      Volume: 10 Pages: 61-232

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Redescription of Triplectides misakianus (Matsumura, 1931) (Trichoptera, Leptoceridae) in Japan with notes on their habitat.2016

    • Author(s)
      KATSUMA, N., R. B. KURANISHI
    • Journal Title

      Zoosymposia

      Volume: 10 Issue: 1 Pages: 234-242

    • DOI

      10.11646/zoosymposia.10.1.21

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] 千葉県立中央博物館生態園におけるヒトスジシマカ成虫の生息状況.2016

    • Author(s)
      木村悟朗・倉西良一
    • Journal Title

      千葉県立中央博物館自然誌研究報告

      Volume: 13 Pages: 77-82

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] 弘前だんぶり池の水生無脊椎動物相2016

    • Author(s)
      木村直哉・小林貞・倉西良一・嶋津武・大高明史
    • Journal Title

      青森自然誌研究

      Volume: 21 Pages: 43-54

    • NAID

      40020814555

    • Related Report
      2016 Research-status Report
  • [Journal Article] 県立中央博物館生態園におけるヒメクロゴキブリの生息状況と食性に関する小観察2016

    • Author(s)
      倉西良一・木村悟朗
    • Journal Title

      房総の昆虫

      Volume: 57 Pages: 106-106

    • Related Report
      2016 Research-status Report
  • [Presentation] On Eubasilissa maclachlani (White, 1862) and its closely related species2018

    • Author(s)
      R.B.Kuranishi
    • Organizer
      16th International Symposium on Trichoptera
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] BAC-FISH identifies the caddisfly chromosomes2018

    • Author(s)
      Fujimoto, T., R. B. Kuranishi, Y. Yasukochi, G. Suzuki, K. Sahara
    • Organizer
      16th International Symposium on Trichoptera
    • Related Report
      2018 Annual Research Report
  • [Presentation] Adult caddisies (Trichoptera) collected by chemical light sticks in the Uji River, Japan2018

    • Author(s)
      Kimura, G., R.B.Kuranishi
    • Organizer
      16th International Symposium on Trichoptera
    • Related Report
      2018 Annual Research Report
  • [Presentation] ムラサキトビケラのタイプ標本の再検討によって分かった事2018

    • Author(s)
      倉西良一
    • Organizer
      日本昆虫学会 第78回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ムラサキトビケラの正体をつきとめる- 欧州タイプ標本探訪記 -2018

    • Author(s)
      倉西良一
    • Organizer
      千葉県昆虫談話会
    • Related Report
      2017 Research-status Report
  • [Presentation] On Eubasilissa maclachlani (White, 1862) and its closely related species2018

    • Author(s)
      Kuranishi, R.B
    • Organizer
      16th International Symposium on Trichoptera
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Adult caddisies (Trichoptera) collected by chemical light sticks in the Uji River, Japan2018

    • Author(s)
      Kimura, G., Kuranishi, R.B
    • Organizer
      16th International Symposium on Trichoptera
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] BAC-FISH identifies the caddisfly chromosomes2018

    • Author(s)
      Fujimoto, T., Kuranishi, R.B., Yasukochi, Y., Suzuki., G., Sahara., K.
    • Organizer
      16th International Symposium on Trichoptera
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 証拠標本の重要性:DNAバーコードの落とし穴2017

    • Author(s)
      倉西良一
    • Organizer
      昆虫DNA研究会 第14回 研究集会
    • Related Report
      2017 Research-status Report 2016 Research-status Report
  • [Presentation] シャープゲンゴロウモドキの遺伝子構造解析2017

    • Author(s)
      倉西良一
    • Organizer
      シャープゲンゴロウモドキ保全協議会
    • Related Report
      2017 Research-status Report
  • [Presentation] ムラサキトビケラ属の分類学的再検討2017

    • Author(s)
      倉西良一
    • Organizer
      日本昆虫学会 第77回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ムラサキトビケラ属 -体サイズに秘められた謎-2017

    • Author(s)
      倉西良一
    • Organizer
      第41回 水生昆虫研究会
    • Related Report
      2017 Research-status Report
  • [Presentation] 北米の自然史博物館で考えた事2017

    • Author(s)
      倉西良一
    • Organizer
      第41回 水生昆虫研究会
    • Related Report
      2017 Research-status Report
  • [Presentation] ムラサキトビケラ - この巨大で不思議な生き物 -2017

    • Author(s)
      倉西良一
    • Organizer
      水生昆虫談話会 423回例会
    • Related Report
      2017 Research-status Report
  • [Presentation] シナノヒゲナガカワトビケラの タイプ標本の再検討2016

    • Author(s)
      倉西良一
    • Organizer
      第40回水生昆虫研究会
    • Place of Presentation
      兵庫県養父市アサヒロッジ
    • Year and Date
      2016-11-26
    • Related Report
      2016 Research-status Report
  • [Presentation] 水生昆虫 液浸標本の整理保存 に関する一考察2016

    • Author(s)
      倉西良一
    • Organizer
      第40回水生昆虫研究会
    • Place of Presentation
      兵庫県養父市アサヒロッジ
    • Year and Date
      2016-11-26
    • Related Report
      2016 Research-status Report
  • [Presentation] ヒゲナガカワトビケラ(ザザムシ)に関する一考察 - 幻のシナノヒゲナガカワトビケラの正体と世界に紹介されたザザムシ - "2016

    • Author(s)
      倉西良一
    • Organizer
      第27回信州昆虫学会大会
    • Place of Presentation
      信州大学理学部
    • Year and Date
      2016-09-03
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Discovery of Eubasilissa signata Wiggins,1998 (Trichoptera, Phryganeidae) from Taiwan2016

    • Author(s)
      Kuranishi, R.B ・HSU Li-peng
    • Organizer
      15th International Symposium on Trichoptera
    • Place of Presentation
      Rutgers University, USA, New Jersey
    • Year and Date
      2016-06-03
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヒゲナガカワトビケラの分子系統地理: 分子・形態・体表炭化水素組成に基づく隠蔽種の発見2016

    • Author(s)
      斎藤梨絵・加藤慎也・野崎隆夫・倉西良一・東城幸治
    • Organizer
      昆虫DNA研究会 第13回研究集会
    • Place of Presentation
      松本・あがたの森文化会館
    • Year and Date
      2016-05-05
    • Related Report
      2016 Research-status Report
  • [Book] 日本昆虫目録第 5巻 ナガレトビケラ科2016

    • Author(s)
      倉西良一
    • Publisher
      櫂歌書房, 福岡市
    • Related Report
      2016 Research-status Report

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Published: 2015-04-16   Modified: 2022-08-22  

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