Understanding of antigen recognition mechanism of multispecific antibodies
| Project/Area Number |
15K07024
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Gifu University |
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Principal Investigator |
KAMATARI Yuji 岐阜大学, 生命科学総合研究支援センター, 助教 (70342772)
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| Co-Investigator(Kenkyū-buntansha) |
織田 昌幸 京都府立大学, 生命環境科学研究科, 准教授 (20318231)
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| Co-Investigator(Renkei-kenkyūsha) |
KUWATA Kazuo 岐阜大学, 連合創薬医療情報研究科, 教授 (00170142)
ISHIGURO Naotaka 岐阜大学, 応用生物科学部, 教授 (00109521)
AKASAKA Kazuyuki 近畿大学, 先端技術総合研究所, 教授 (50025368)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | タンパク質 / 抗体 / 抗原認識機構 / 揺らぎ / 準安定状態 / NMR |
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| Outline of Final Research Achievements |
In general, a monoclonal antibody (mAb) bind only one antigen (Ag). However, we found an mAb G2 capable of specifically recognizing multiple Ags. The purpose of this study is to understand the Ag recognition mechanism of G2. This study suggested that the Ag-binding site of G2 was flexible in the free form and capable of adapting to at least three different conformations to enable interactions with three different Ags. Further development of this research may lead to extension of the concept of Ag recognition of mAbs, understanding of molecular evolution of proteins, and creation of novel antibody medicine that inhibits two pathways at the same time.
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Report
(4 results)
Research Products
(45 results)
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[Journal Article] Molecular basis for diversification of yeast prion strain conformation2018
Author(s)
Ohhashi Yumiko、Yamaguchi Yoshiki、Kurahashi Hiroshi、Kamatari Yuji O.、Sugiyama Shinju、Uluca Boran、Piechatzek Timo、Komi Yusuke、Shida Toshinobu、M?ller Henrik、Hanashima Shinya、Heise Henrike、Kuwata Kazuo、Tanaka Motomasa
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Journal Title
Proceedings of the National Academy of Sciences
Volume: 115
Issue: 10
Pages: 2389-2394
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Instability of C154Y variant of aldo-keto reductase 10.1038/s41598-017-00710-x1C32017
Author(s)
Satoshi Endo, Sayaka Takada, Ryo P. Honda, Kathrin Muller, Jochen H. Weishaupt, Peter M. Andersen, Albert C. Ludolph, Yuji O. Kamatari, Toshiyuki Matsunaga, Kazuo Kuwata, Ossama El-Kabbani, Akira Ikari
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Journal Title
Chemico-Biological Interactions
Volume: S0009-2797(16)
Pages: 194-202
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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