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Development of luminescent prove for inter-cellular communications

Research Project

Project/Area Number 15K07040
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Cell biology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

SAITO Kenta  東京医科歯科大学, 大学院医歯学総合研究科, 助教 (60374659)

Co-Investigator(Renkei-kenkyūsha) TERADA Sumio  東京医科歯科大学, 大学院医歯学総合研究科, 教授 (00262022)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords細胞間相互作用 / 神経シナプス / 細胞生物学 / 機能性バイオプローブ / バイオイメージング / BRET / FRET / 細胞間情報伝達
Outline of Final Research Achievements

In this research, I have developed the functional probe to visualize neural synapse, which is a typical inter-cellular communication in multi-cellular organisms. By using the molecules specifically localizing in pre- or post-synapse, fluorescent or bio-luminescent molecules were exposed in synapse. Probe was optimized by assessing the signal strength at contact sites between probe expressing culture cells. The optimized probe successfully visualized the synapse in mouse hippocampal primary culture neurons. Signal formation and dis-formation were seen in time-lapse imaging. Synaptic signal transduction from pre- to post-synapse at signal site was confirmed by optogenetics.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (5 results)

All 2018 2015

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (1 results)

  • [Journal Article] Expanded palette of Nano-lantern for real-time muliti-color luminescence imaging2015

    • Author(s)
      Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin T, Okada Y, Nagai T
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 112 Issue: 14 Pages: 4352-4356

    • DOI

      10.1073/pnas.1418468112

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Visible-wavelength two-photon excitation microscopy for fluorescent protein imaging.2015

    • Author(s)
      M. Yamanaka, K. Saito, N.I. Smith, Y. Arai, K. Uegaki, Y. Yonemaru, K. Mochizuki, S. Kawata, T. Nagai, K. Fujita
    • Journal Title

      Journal of Biomedical Optics

      Volume: 20 Issue: 10 Pages: 101202-101202

    • DOI

      10.1117/1.jbo.20.10.101202

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Recent progress in luminescent proteins development.2015

    • Author(s)
      K. Saito, T. Nagai
    • Journal Title

      Current Opinion in Chemical Biology

      Volume: 27 Pages: 46-51

    • DOI

      10.1016/j.cbpa.2015.05.029

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Real Time Imaging of Biological Phenomena with Super-duper Luminescent Proteins2015

    • Author(s)
      Sakai A, Takusagawa M, Nio A, Sawai Y
    • Journal Title

      CYTOLOGIA

      Volume: 80 Issue: 1 Pages: 1-2

    • DOI

      10.1508/cytologia.80.1

    • NAID

      130004801668

    • ISSN
      0011-4545, 1348-7019
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 神経シナプスの動態を可視化する新規プローブの開発2018

    • Author(s)
      齊藤健太、寺田純雄
    • Organizer
      日本解剖学会
    • Related Report
      2017 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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