Molecular basis of periodic cellular dynamics to facilitate convergence movements

• Project/Area Number: 15K07088
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: National Institute for Basic Biology
• Principal Investigator: Suzuki Makoto 基礎生物学研究所, 形態形成研究部門, 助教 (10533193)
• Research Collaborator: UENO Naoto ; TAKADA Shinji ; YABE Taijirou
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 収斂運動 / ゼブラフィッシュ / アクトミオシン / 神経管 / 低分子GTP結合タンパク質

Outline of Final Research Achievements

The purpose of this study is to clarify the significance and the molecular mechanism of periodic cellular movements observed in convergence movements, a typical example of collective cell movement in animal development. The results from the analyses using zebrafish embryos suggest that the periodic and isotropic contraction of actomyosin in the cell cortex leads to the anisotropic cellular movement through the interaction between the cellular protrusion and the extracellular matrix on the leading edge, which ensures robustness of the convergent movements during neural tube formation.

Academic Significance and Societal Importance of the Research Achievements

アクトミオシン骨格と細胞の挙動の周期性は他の器官形成過程でも報告が相次いでおり、その新たな意義を明らかにした本研究の成果は動物に保存された集団的細胞運動の基本原理の理解に繋がることが期待される。また細胞の集合に基づく神経管の形成機構は哺乳類胚でも尾部領域で観察されることから、本研究の成果の更なる発展は神経管の形成異常により発症するヒト先天異常である神経管閉鎖障害の病態理解に貢献することが期待される。

Research Products

• [Journal Article] Elasticity-based boosting of neuroepithelial nukleokinesis via indirect energy transfer from mother to daughter (2018)
  • Author(s): Tomoyasu Shinoda, Arata Nagasaka, Yasuhiro Inoue, Ryo Higuchi, Yoshiaki Minami, Kagayaki Kato, Makoto Suzuki, Takefumi Kondo, Takumi Kawaue, Kanako Saito, Naoto Ueno, Yugo Fukazaawa, Masaharu Nagayama, Takashi Miura, Taiji Adachi, Takaki Miyata
  • Journal Title: PLoS Biology Volume: 16 Issue: 4 Pages: 2004426-2004426
  • DOI: 10.1371/journal.pbio.2004426
  • Peer Reviewed / Open Access
• [Journal Article] ZNRF3 functions in mammalian sex determination by inhibiting canonical WNT signaling (2018)
  • Author(s): Harris A.、Siggers P.、Corrochano S.、Warr N.、Sagar D.、Grimes D.T.、Suzuki M.、Burdine R.D.、Cong F.、Koo B-K.、Clevers H.、Stevant I.、Nef S.、Wells S.、Brauner R.、Ben R.B.、Belguith N.、Eozenou C.、Bignon-Topalovic J.、Bashamboo A.、McElreavey K.、Greenfield A.
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 115 Issue: 21 Pages: 5474-5479
  • DOI: 10.1073/pnas.1801223115
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Skin regeneration of amphibians: A novel model for skin regeneration as adults (2018)
  • Author(s): Yokoyama Hitoshi、Kudo Nanako、Todate Momoko、Shimada Yuri、Suzuki Makoto、Tamura Koji
  • Journal Title: Development, Growth & Differentiation Volume: 60 Issue: 6 Pages: 316-325
  • DOI: 10.1111/dgd.12544
  • Peer Reviewed
• [Journal Article] Mechanical regulation of three-dimensional epithelial fold pattern formation in the mouse oviduct (2016)
  • Author(s): Koyama, H., Shi, D., Suzuki, M., Ueno, N., Uemura, T., Fujimori, T.
  • Journal Title: Biophysical Journal Volume: 111 Issue: 3 Pages: 650-665
  • DOI: 10.1016/j.bpj.2016.06.032
  • Peer Reviewed
• [Journal Article] Mechanical roles of apical constriction, cell elongation, and cell migration during neural tube formation in Xenopus, Biomechanics and Modeling in Mechanobiology (2016)
  • Author(s): Yasuhiro Inoue, Makoto Suzuki, Tadashi Watanabe, Naoko Yasue, Itsuki Tateo, Taiji Adachi, Naoto Ueno
  • Journal Title: Biomechanics and Modeling in Mechanobiology Volume: 15 Issue: 6 Pages: 1733-1746
  • DOI: 10.1007/s10237-016-0794-1
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Differences in the Mechanical Properties of the Developing Cerebral Cortical Proliferative Zone between Mice and Ferrets at both the Tissue and Single-Cell Levels. (2016)
  • Author(s): Nagasaka, A., Shinoda, T., Kawaue, T., Suzuki, M., Nagayama, K., Matsumoto, T., Ueno, N., Kawaguchi, A. and Miyata, T.
  • Journal Title: Front Cell Dev. Biol. Volume: 25 Pages: 139-139
  • DOI: 10.3389/fcell.2016.00139
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Mechanical regulation of folding and tubulogenesis of Xenopus neural plate (2018)
  • Author(s): Suzuki, M., Inoue, Y. & Ueno, N.
  • Organizer: 第70回日本細胞生物学会・第51回日本発生生物学会合同大会
  • Invited
• [Presentation] 脳の発生からみる上皮組織の折りたたみと管形成の力学制御 (2018)
  • Author(s): 鈴木 誠
  • Organizer: 第41回日本バイオレオロジー学会年会
  • Invited
• [Presentation] Mechanical regulation of closing movement and morphology of Xenopus neural tube (2018)
  • Author(s): Suzuki, M., Yasue, N. & Ueno, N.
  • Organizer: 17th International Xenopus conference
  • Int'l Joint Research
• [Presentation] Mechanical regulation of epithelial folding and tubulogenesis of neural tube (2018)
  • Author(s): Suzuki, M.
  • Organizer: 12th GfE School “Imaging and Modeling Development”
  • Int'l Joint Research
• [Presentation] 神経管の閉鎖運動と形状決定の力学制御 (2018)
  • Author(s): 鈴木 誠, 上野 直人
  • Organizer: 第12回日本ツメガエル研究集会・第4回次世代両生類研究会合同シンポジウム
• [Presentation] 神経管の閉鎖運動と形状決定の力学制御 (2018)
  • Author(s): 鈴木 誠
  • Organizer: 日本発生生物学会秋季シンポジウム2018
• [Presentation] 動物発生のバイオメカニクス (2017)
  • Author(s): 上野直人, 鈴木誠, 根岸剛文.
  • Organizer: 日本機械学会第29回バイオエンジニアリング講演会
  • Place of Presentation: ウインクあいち（愛知県・名古屋市）
  • Year and Date: 2017-01-19
  • Invited
• [Presentation] Periodic actomyosin contractility contributes to convergence movement during neurulation in zebrafish (2017)
  • Author(s): Makoto Suzuki, Naoto Ueno.
  • Organizer: 18th International Congress of Developmental Biology
  • Int'l Joint Research
• [Presentation] Distinct intracellular Ca2+ dynamics regulate apical constriction and differentially contribute to neural tube closure (2017)
  • Author(s): Makoto Suzuki, Masanao Sato, Hiroshi Koyama, Naoto Ueno.
  • Organizer: 第27回日本数理生物学会年会
  • Invited
• [Presentation] Distinct intracellular calcium ion dynamics regulate apical constriction and differentially con- tribute to neural tube closure (2016)
  • Author(s): Suzuki, M., Ueno, N.
  • Organizer: The 22nd International Congress of Zoology (OIST, Okinawa)
  • Place of Presentation: 沖縄科学技術大学院大学（沖縄県、恩納村）
  • Int'l Joint Research / Invited