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Biological roles of protein complexes of NAC domain transcription factors

Research Project

Project/Area Number 15K07094
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionSaitama University

Principal Investigator

Yamaguchi Masatoshi  埼玉大学, 理工学研究科, 准教授 (20373376)

Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsNACドメイン転写因子 / 植物 / タンパク質複合体 / 転写因子 / タンパク質相互作用 / シロイヌナズナ / ユビキチンE3ライゲース
Outline of Final Research Achievements

NAC domain transcription factor is known to be involved in various biological processes. In this study, to understand the protein complexes of NAC domain transcription factors, we focused on VNI2, isolated as an interacting protein with a key regulator of xylem vessel differentiation, VND7. We found that ATAF2 forms complex with VNI2, ATAF2 plays roles in promotion of senescence, and VNI2 inhibits ATAF2 function, resulting in repressing senescence. These findings provide an insight that NAC domain transcription factors are able to play roles in various physiological events by forming hetero-dimer complexes with other different NAC domain transcription factors.

Academic Significance and Societal Importance of the Research Achievements

植物の成長は、生育環境を回避することができないので、刻々と変化する環境に柔軟に対応しながら成長することが求められている。このような柔軟な成長様式は、様々な制御機構が互いに影響を及ぼし合うことで成立すると考えられる。今回私たちは、NACドメイン転写因子は結合するターゲットが変わることで、異なる制御機構に関与することを明らかにした。このことは、NACドメイン転写因子は、様々な組み合わせで複合体を形成することで、より多面的な機能を発揮しうることを示唆している。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (36 results)

All 2019 2018 2017 2016 2015 Other

All Int'l Joint Research (2 results) Journal Article (10 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 10 results,  Open Access: 8 results,  Acknowledgement Compliant: 2 results) Presentation (23 results) (of which Int'l Joint Research: 6 results,  Invited: 2 results) Remarks (1 results)

  • [Int'l Joint Research] The University of British Columbia(Canada)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] The University of British Columbia(Canada)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Arabidopsis Group IIId ERF proteins positively regulate primary cell wall-type CESA genes2019

    • Author(s)
      Saelim Laddawan、Akiyoshi Nobuhiro、Tan Tian Tian、Ihara Ayumi、Yamaguchi Masatoshi、Hirano Ko、Matsuoka Makoto、Demura Taku、Ohtani Misato
    • Journal Title

      Journal of Plant Research

      Volume: 132 Issue: 1 Pages: 117-129

    • DOI

      10.1007/s10265-018-1074-1

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] One of the NAD kinases, sll1415, is required for the glucose metabolism of Synechocystis sp. PCC 68032019

    • Author(s)
      Ishikawa Y., Miyagi A., Ishikawa T., Nagano M., Yamaguchi M., Hihara Y., Kaneko Y., Kawai-Yamada M.
    • Journal Title

      Plant J.

      Volume: - Issue: 4 Pages: 654-666

    • DOI

      10.1111/tpj.14262

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] An NAC domain transcription factor ATAF2 acts as transcriptional activator or repressor dependent on promoter context2018

    • Author(s)
      Nagahage Isura Sumeda Priyadarshana、Sakamoto Shingo、Nagano Minoru、Ishikawa Toshiki、Kawai-Yamada Maki、Mitsuda Nobutaka、Yamaguchi Masatoshi
    • Journal Title

      Plant Biotechnology

      Volume: 35 Issue: 3 Pages: 285-289

    • DOI

      10.5511/plantbiotechnology.18.0507a

    • NAID

      130007495601

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2018-09-25
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization of rice KT/HAK/KUP potassium transporters and K<sup>+</sup> uptake by HAK1 from <i>Oryza sativa</i>2018

    • Author(s)
      Okada Tomoyuki、Yamane Sousuke、Yamaguchi Masatoshi、Kato Ko、Shinmyo Atsuhiko、Tsunemitsu Yuta、Iwasaki Kozo、Ueno Daisei、Demura Taku
    • Journal Title

      Plant Biotechnology

      Volume: 35 Issue: 2 Pages: 101-111

    • DOI

      10.5511/plantbiotechnology.18.0308a

    • NAID

      130007393718

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2018-06-25
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evaluation of metabolic changes in oxalate-rich plant Rumex obtusifolius L. caused by ion beam irradiation2018

    • Author(s)
      Miyagi Atsuko、Kitano Sayaka、Oono Yutaka、Hase Yoshihiro、Narumi Issay、Yamaguchi Masatoshi、Uchimiya Hirofumi、Kawai-Yamada Maki
    • Journal Title

      Plant Physiology and Biochemistry

      Volume: 122 Pages: 40-45

    • DOI

      10.1016/j.plaphy.2017.11.001

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Heterologous expression and characterization of an Arabidopsis β-l-arabinopyranosidase and α-d-galactosidases acting on β-l-arabinopyranosyl residues2017

    • Author(s)
      Imaizumi Chiemi、Tomatsu Harumi、Kitazawa Kiminari、Yoshimi Yoshihisa、Shibano Seiji、Kikuchi Kaoru、Yamaguchi Masatoshi、Kaneko Satoshi、Tsumuraya Yoichi、Kotake Toshihisa
    • Journal Title

      Journal of Experimental Botany

      Volume: 68 Issue: 16 Pages: 4651-4661

    • DOI

      10.1093/jxb/erx279

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transcription Factors VND1-VND3 Contribute to Cotyledon Xylem Vessel Formation2017

    • Author(s)
      Tan Tian Tian、Endo Hitoshi、Sano Ryosuke、Kurata Tetsuya、Yamaguchi Masatoshi、Ohtani Misato、Demura Taku
    • Journal Title

      Plant Physiology

      Volume: 176 Issue: 1 Pages: 773-789

    • DOI

      10.1104/pp.17.00461

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Metabolomic analysis of NAD kinase-deficient mutants of the cyanobacterium Synechocystis sp. PCC 68032016

    • Author(s)
      Ishikawa Y, Miyagi A, Haishima Y, Ishikawa T, Nagano M, Yamaguchi M, Hihara Y, Kawai-Yamada M
    • Journal Title

      Journal of Plant Physiology

      Volume: 205 Pages: 105-112

    • DOI

      10.1016/j.jplph.2016.09.002

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] NAC-MYB-based transcriptional regulation of secondary cell wall biosynthesis in land plants.2015

    • Author(s)
      Nakano Y, Yamaguchi M, Endo H, Rejab NA, Ohtani M
    • Journal Title

      Frontiers in Plant Science

      Volume: 6 Pages: 288-288

    • DOI

      10.3389/fpls.2015.00288

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The expression of a rice secondary wall-specific cellulose synthase gene, OsCesA7, is directly regulated by a rice transcription factor, OsMYB58/632015

    • Author(s)
      Noda S, Koshiba T, Hattori T, Yamaguchi M, Suzuki S, Umezawa T
    • Journal Title

      Planta

      Volume: 242 Issue: 3 Pages: 589-600

    • DOI

      10.1007/s00425-015-2343-z

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] シアノバクテリアSynechocystis sp.PCC6903が有するNADキナーゼの生理的な役割2019

    • Author(s)
      石川優真、宮城敦子、石川寿樹、長野稔、山口雅利、園池公毅、日原由香子、金子康子、川合真紀
    • Organizer
      第60回日本植物生理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 相互作用因子による道管形成抑制因子VNI2のタンパク質分解制御2018

    • Author(s)
      山田拓矢、北川純子、松田浩平、長野稔、石川寿樹、川合真紀、出村拓、山口雅利
    • Organizer
      第36回日本植物細胞分子生物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] シロイヌナズナVND遺伝子群の遺伝学的解析2018

    • Author(s)
      伊藤敦也、久保稔、大谷美紗都、石川寿樹、川合真紀、出村拓、山口雅利
    • Organizer
      第36回日本植物細胞分子生物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] シアノバクテリアにおけるNADキナーゼの異なる役割2018

    • Author(s)
      石川優真、宮城敦子、石川寿樹、長野稔、山口雅利、園池公毅、日原由香子、金子康子、川合真紀
    • Organizer
      第82回日本植物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 転写抑制因子VNI2は繊維細胞マスター因子NST1, NST3の機能を抑制する2018

    • Author(s)
      山口雅利、坂本真吾、川合真紀、光田展隆
    • Organizer
      第81回日本植物学会
    • Related Report
      2017 Research-status Report
  • [Presentation] シアノバクテリアSynecocystis sp. PC6803におけるNADキナーゼの異なる役割2018

    • Author(s)
      石川優真、宮城敦子、長野稔、石川寿樹、山口雅利、園池公毅、日原由香子、金子康子、川合真紀
    • Organizer
      第59回日本植物生理学会
    • Related Report
      2017 Research-status Report
  • [Presentation] NAC domain transcription factor VNI2 regulates senescence by forming protein complexes2018

    • Author(s)
      Isura Nagahage、松田浩平、坂本真吾、石川寿樹、長野稔、光田展隆、川合真紀、出村拓、山口雅利
    • Organizer
      第59回日本植物生理学会
    • Related Report
      2017 Research-status Report
  • [Presentation] メタボローム解析によるシアノバクテリアNADキナーゼの機能解明2017

    • Author(s)
      石川優真、宮城敦子、長野稔、石川寿樹、山口雅利、金子康子、日原由香子、川合真紀
    • Organizer
      第35回日本植物細胞分子生物学会
    • Related Report
      2017 Research-status Report
  • [Presentation] イオンビーム育種による低シュウ酸イネの作出2017

    • Author(s)
      宮城敦子、西丸拓也、針谷のぞみ、大野豊、長谷純宏、長野稔、石川寿樹、山口雅利、川合真紀
    • Organizer
      第35回日本植物細胞分子生物学会
    • Related Report
      2017 Research-status Report
  • [Presentation] 道管形成を負に制御する転写因子VNI2のタンパク質制御機構2017

    • Author(s)
      山田拓矢、北川純子、松田浩平、長野稔、石川寿樹、川合真紀、出村拓、山口雅利
    • Organizer
      第35回日本植物細胞分子生物学会
    • Related Report
      2017 Research-status Report
  • [Presentation] NAC domain transcription factors, VNI2 and ATAF2 form a protein complex2017

    • Author(s)
      Isura Nagahage、Kohei Matsuda、Shingo Sakamoto、Minoru Nagano、Toshiki Ishikawa、Nobutaka Mitsuda、Maki Kawai-Yamada、Taku Demura、Masatoshi Yamaguchi
    • Organizer
      第35回日本植物細胞分子生物学会
    • Related Report
      2017 Research-status Report
  • [Presentation] 植物スフィンゴ脂質不飽和化酵素の機能解析2017

    • Author(s)
      葛葉修平、石川寿樹、長野稔、山口雅利、川合真紀
    • Organizer
      第35回日本植物細胞分子生物学会
    • Related Report
      2017 Research-status Report
  • [Presentation] イネにおけるICLの機能解析2017

    • Author(s)
      宮城敦子、西丸拓也、尾﨑莉沙子、長野稔、石川寿樹、山口雅利、川合真紀
    • Organizer
      第81回日本植物学会
    • Related Report
      2017 Research-status Report
  • [Presentation] 2-ヒドロキシスフィンゴ脂質によるシロイヌナズナ耐病性の解析2017

    • Author(s)
      宇川智水、長野稔、石川寿樹、山口雅利、川合真紀
    • Organizer
      第81回日本植物学会
    • Related Report
      2017 Research-status Report
  • [Presentation] Transcription factors regulating secondary cell wall formation2017

    • Author(s)
      Masatoshi Yamaguchi
    • Organizer
      Workshop on Plant Glycobiology
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] NACドメイン転写因子VNI2とその相互作用因子の機能解析2016

    • Author(s)
      山口雅利、Isura Nagahage、松田浩平、山田拓矢、北川純子、坂本真吾、光田展隆、長野稔、石川寿樹、内宮博文、川合真紀、出村拓
    • Organizer
      日本植物学会第80回大会
    • Place of Presentation
      沖縄コンベンションセンター、沖縄県宜野湾市
    • Year and Date
      2016-09-16
    • Related Report
      2016 Research-status Report
  • [Presentation] 転写抑制因子VNI2を活用した木質バイオマスの量的改変2016

    • Author(s)
      山口雅利、坂本真吾、川合真紀、出村拓、内宮博文、光田展隆
    • Organizer
      第34回日本植物細胞分子生物学会
    • Place of Presentation
      信州大学、長野県上田市
    • Year and Date
      2016-09-01
    • Related Report
      2016 Research-status Report
  • [Presentation] Ectopic expression of a transcription factor modulates secondary cell wall content in fiber cells2016

    • Author(s)
      Yamaguchi M, Nakamoto S, Kawai-Yamada M, Demura T, Uchimiya H, Mitsuda N
    • Organizer
      XIV Cell Wall Meeting
    • Place of Presentation
      Crete, Greece
    • Year and Date
      2016-06-12
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Stability of VNI2 protein is regulated by an interacting protein2015

    • Author(s)
      Yamaguchi M, Kitagawa J, Uchimiya H, Kawai-Yamada M, Demura T
    • Organizer
      The Canadian Society of Plant Biologists Eastern Regional Meeting
    • Place of Presentation
      Toronto, Canada
    • Year and Date
      2015-11-20
    • Related Report
      2015 Research-status Report
  • [Presentation] A transcriptional repressor, VNI2, modulates secondary cell wall content2015

    • Author(s)
      Yamaguchi M, Sakamoto S, Kawai-Yamada M, Demura T, Uchimiya H, Mitsuda N
    • Organizer
      11th International Congress of Plant Molecular Biology
    • Place of Presentation
      Igaucu, Brazil
    • Year and Date
      2015-10-25
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Transcriptional regulation during primary and secondary cell wall formation2015

    • Author(s)
      Ohtani M, Endo H, Laddawan S, Yamaguchi M, Kubo M, Demura T
    • Organizer
      11th International Congress of Plant Molecular Biology
    • Place of Presentation
      Igaucu, Brazil
    • Year and Date
      2015-10-25
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] NAC domain transcription factors interacting with VND7, a key regulator of xylem vessel differentiation2015

    • Author(s)
      Yamaguchi M, Nagahage I, Ohtani M, Ishikawa T, Uchimiya H, Fukuda H, Kawai-Yamada M, Demura T
    • Organizer
      Botany 2015, Science and Plants for People
    • Place of Presentation
      Edmonton, Canada
    • Year and Date
      2015-07-25
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Stability of VNI2 protein is regulated by RING finger proteins2015

    • Author(s)
      Yamaguchi M, Kitagawa J, Matsuda K, Uchimiya H, Kawai-Yamada M, Demura T
    • Organizer
      The 26th International Conference on Arabidopsis Research
    • Place of Presentation
      Paris, France
    • Year and Date
      2015-07-05
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Remarks] 埼玉大学大学院理工学研究科 山口雅利研究グループHP

    • URL

      http://park.saitama-u.ac.jp/~myamaguchi/index.html

    • Related Report
      2018 Annual Research Report

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Published: 2015-04-16   Modified: 2020-03-30  

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