Studies on tip specific blue light perception and IAA biosynthesis/transport in maize coleoptiles

• Project/Area Number: 15K07111
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Tokyo Metropolitan University
• Principal Investigator: KOSHIBA Tomokazu 首都大学東京, 理学研究科, 客員教授 (80117704)
• Co-Investigator(Renkei-kenkyūsha): OKAMOTO takashi ; YOKAWA ken
• Research Collaborator: SUZUKI hiromi ; BALUSKA Frantisek
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 植物生理学 / 植物ホルモン / 植物環境応答 / 幼葉鞘の光屈曲 / 根の光・重量応答 / 根系分化・成長 / 根の光・重力応答

Outline of Final Research Achievements

We have obtained several results as followers.
（1）Blue light specific site (cells) in maize coleoptiles are suggested to be outer cell layers of coleoptile regions, which are indicated IAA synthesis regions as previous studies. (2) Movement of starch grains in the root tip cells are suggested to be regulate light irradiation, and necessary in light-dependent root gravitropism. (3) Ser291 in Zmphot1 was identified as a low-fluence blue light-induced phosphorylation site that is crucial for the first positive phototropism. (4) RSOsPR10 expression in rice root tips are regulated by cross-talk of plant hormones, such as jasmonate, salicylic acid and maybe IAA. Main results of these studies have been published in international journals.

Academic Significance and Societal Importance of the Research Achievements

植物の光屈性の分子レベルの機構に関して新たな知見を得ることができた。一部は、まだ論文投稿中の内容があるが、当初の目標を十分に達成した。今後の世界的な研究の進展に大きく貢献するものである。

Research Products

• [Int'l Joint Research] IZMB/University of Bonn(Germany)
• [Int'l Joint Research] IZMB/University of Bonn(Germany)
• [Int'l Joint Research] University of Bonn(Germany)
• [Journal Article] Expression of RSOsPR10 in rice roots is antagonistically regulated by jasmonate/ethylene and salicylic acid via the activator OsERF87 and the repressor OsWRKY76, respectively (2018)
  • Author(s): Yamamoto, T., Yoshida, Y., Nakajima, K., Tominaga, M., Gyohda, A., Suzuki, H., Okamoto, T., Nishimura, T., Yokotani, N., Minami, E., Nishizawa, Y., Miyamoto, K., Yamane, H., Okada, K., Koshib, T.
  • Journal Title: Plant Direct Volume: ３ Issue: 3 Pages: 1-17
  • DOI: 10.1002/pld3.49
  • Peer Reviewed / Open Access
• [Journal Article] AtSWEET13 and AtSWEET14 regulate gibberellin-mediated physiological processes (2016)
  • Author(s): Y. Kanno, T. Oikawa, Y. Chiba, Y. Ishimaru, T. Shimizu, N. Sano, T. Koshiba, Y. Kamiya, M. Ueda, M. Seo
  • Journal Title: Nature Commun. Volume: 7 Issue: 1 Pages: 1-11
  • DOI: 10.1038/ncomms13245
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Overexpression of RSOsPR10, a root-specific rice PR10 gene, confers tolerance against drought stress in rice and drought and salt stresses in bentgrass. (2016)
  • Author(s): Takeuchi, K., Hasegawa, H., Gyohda, A., Komatsu, S., Okamoto, T., Okada, K., Terakawa, T., Koshiba, T.
  • Journal Title: Plant Cell Tissue Organ Cult. Volume: 127 Issue: 1 Pages: 35-46
  • DOI: 10.1007/s11240-016-1027-0
  • Peer Reviewed / Open Access
• [Journal Article] Root cap-dependent gravitropic U-turn of maize root requires light-induced de novo auxin biosynthesis via YUC-pathway in root apex (2016)
  • Author(s): Suzuki H, Yokawa K, Nakano S, Yoshida Y, Fabrissin I, Okamoto T, Baluska F, Koshiba T
  • Journal Title: Journal of Experimental Botany Volume: 67 Issue: 15 Pages: 4581-4591
  • DOI: 10.1093/jxb/erw232
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Effects of anti-auxins on secondary aerenchyma formation in flooded soybean hypocotyls (2016)
  • Author(s): Satoshi Shimamura, Takeshi Nishimura, Tomokazu Koshiba, Ryo Yamamoto, Susumu Hiraga, Takuji Nakamura, Setsuko Komatsu
  • Journal Title: Plant Production Science Volume: 19 Issue: 1 Pages: 154-160
  • DOI: 10.1080/1343943x.2015.1128101
  • Peer Reviewed
• [Journal Article] Live single cell plant hormone analysis by video-mass spectrometry. (2015)
  • Author(s): Shimizu, T., S. Miyakawa, T. Esaki, H. Mizuno, T. Masujima, T. Koshiba and M. Seo.
  • Journal Title: Plant Cell Physiol. Volume: 56 Issue: 7 Pages: 1-6
  • DOI: 10.1093/pcp/pcv042
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Identification of Arabidopsis thaliana NRT1/PTR FAMILY (NPF) proteins capable of transporting plant hormones. (2015)
  • Author(s): Chiba, Y., Shimizu, T., Miyakawa, S., Kanno, Y., Koshiba, T., Kamiya, Y., Seo, M.
  • Journal Title: J Plant Res Volume: 128 Issue: 4 Pages: 679-686
  • DOI: 10.1007/s10265-015-0710-2
  • Peer Reviewed
• [Presentation] Zmphot1 functions according to the extent of its fluence-dependent phosphorylation (2018)
  • Author(s): Suzuki , H., Koshiba, T., Fujita, C., Yamauchi, Y., Kimura, T., Isobe, T., Sakai, T., Taoka, M., Okamoto, T.
  • Organizer: 第59回日本植物生理学会年会
• [Presentation] Identification of Arabidopsis SWEET proteins capable of transporting gibberellins (2018)
  • Author(s): Kanno, Y., Oikawa, T., Chiba, Y., Ishimaru, Y., Shimizu, T., Sano, N., Koshiba, T., Kamiya, K., Seo, M.
  • Organizer: 第59回日本植物生理学会年会
• [Presentation] Analysis of fluense-dependent phosphorylation of Zmphot1 in phototropism of maize coleoptiles. (2017)
  • Author(s): Suzuki H., Fujita C., Kimura T., Sakai T., Isobe T., Taoka M., Okamoto T., Koshiba T.
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学 鹿児島県
  • Year and Date: 2017-03-16
• [Presentation] Current situation of quantitative single cell plant hormone analysis by mass spectrometry. (2017)
  • Author(s): Shimizu T., Masujima, T., Koshiba, T., Seo, M.
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学 鹿児島県
  • Year and Date: 2017-03-16
• [Presentation] トウモロコシ幼根の重力屈性には光照射によるIAAの新規合成が必要である (2016)
  • Author(s): 陽川憲、鈴木洋弥、中野紗佑里、吉田侑里子、岡本龍史、Baluska Frantisek、小柴共一
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学 岩手県
  • Year and Date: 2016-03-18
• [Presentation] トウモロコシ幼葉鞘におけるZmphot1のリン酸化状態への青色光の影響 (2016)
  • Author(s): 鈴木洋弥、礒辺俊明、藤田千春、田岡万悟、岡本龍史、小柴共一
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学 岩手県
  • Year and Date: 2016-03-18
• [Presentation] Overexpression of RSOsPR10 improves drought torelance in rice and drought and salt torelance in bentgrass. (2015)
  • Author(s): Koshiba T.
  • Organizer: 7th International Symposium on Frontiers in Agricultural Proteome Research,and 2nd AOAPO conference in Korea,2015
  • Place of Presentation: Goesan-gun,Chungcheongbuk-do,Korea
  • Year and Date: 2015-09-23
  • Int'l Joint Research / Invited
• [Presentation] Subcellular localization and interaction of Zmphot1 and ZmNPH3-like proteins. (2015)
  • Author(s): Suzuki, H., Kanegae, T., Okamoto, T., Koshiba
  • Organizer: International Conference on Arabidopsis Research
  • Place of Presentation: Paris, France
  • Year and Date: 2015-07-05
  • Int'l Joint Research / Invited
• [Presentation] Identification of plant hormone transporters by a new approach. (2015)
  • Author(s): Kanno,Y., Shimizu,T., Chiba,Y., Miyakawa,S., Sano,N., Koshiba,T., Kamiya,Y., Seo,M.
  • Organizer: International Conference on Arabidopsis Research
  • Place of Presentation: Paris, France
  • Year and Date: 2015-07-05
  • Int'l Joint Research
• [Presentation] Overexpression of root specific rice [rsosPR10]confers tolerance against drought and salt in race and grass plants (2015)
  • Author(s): Gyoda A,Hasegawa H,Komatsu S,Okamoto T,Okada K,Terakawa T,Koshiba T
  • Organizer: International Symposium on Plant Signaling and Behavior
  • Place of Presentation: Paris, France
  • Year and Date: 2015-06-29
  • Int'l Joint Research
• [Presentation] Light and root cap-dependent gravitropism of maize root requires auxin biosynthesis. (2015)
  • Author(s): Suzuki, H., Yokawa K., Okamoto T., Baluška, F., Koshiba, T.
  • Organizer: International Symposium on Plant Signaling and Behavior
  • Place of Presentation: Paris, France
  • Year and Date: 2015-06-29
  • Int'l Joint Research