Evolutionary ecology of asexual green alga Ulva prolifera including many heterozygous genotypes

• Project/Area Number: 15K07194
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biodiversity/Systematics
• Research Institution: Tokyo University of Marine Science and Technology (2018); Fukui Prefectural University (2015-2017)
• Principal Investigator: Kamiya Mitsunobu 東京海洋大学, 学術研究院, 教授 (00281139)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2017: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
• Keywords: 種分化 / 生理的分化 / 走光性 / ヘテロ接合 / 無性生殖 / 遺伝的多型 / 乾燥耐性 / ヘテロ接合度 / 無性生殖化 / 交雑 / 塩濃度 / 生態型 / 生理 / 淡水耐性

Outline of Final Research Achievements

Eight hsp90 genotypes of Ulva prolifera mainly detected from Mikata-goko and Minami River were distinguished into four ecotypes based on their distribution patterns: type I found throughout Mikata-goko, type II distributed in the downstream and entrance of Mikata-goko, type III observed in the downstream and entrance of Mikata-goko and Minami River, and type IV limited in Minami River. Tolerance against freshwater exposure and maturation rate in a low salinity medium were different among these ecotypes, suggesting their physiological differentiation. Furthermore, the examination of settled zoids in using hsp90 gene markers revealed that zoids of type I were detected from the entrance of Mikata-goko where thalli of type I had never been observed. Although zoids of various ecotypes were widely dispersed, the zoids of only ecotype(s) adapted to each environmental condition were survived under selection pressure, and this makes the distribution pattern of each ecotype different.

Academic Significance and Societal Importance of the Research Achievements

大型藻類の遺伝子解析が進み、様々な分類群で種内の遺伝的多型が検出されるとともに、遺伝子型（ハプロタイプ）の組成や頻度が地域によって異なることが明らかになってきた。しかしながら、その違いがどのような要因で生じ、維持されているのかほとんど分かっていない。本研究は、種内の遺伝的差異だけでなく、生理・生態的多型の存在を示すとともに、分布パターンの違いが維持される仕組みを明らかにした点で、意義深いものと考えられる。今後、同様な手法を用いて、様々な大型藻類について生理・生態的特性の解析や種分化プロセスの機構解明が進むことが期待される。

Research Products

• [Journal Article] Proposal of a new brown algal species Mesogloia japonica sp. nov. (Chordariaceae, Phaeophyceae) and transfer of Sauvageaugloia ikomae to Mesogloia (2019)
  • Author(s): Kawai, H., Hanyuda, T., Shibata, K., Kamiya, M., Peters, A.F.
  • Journal Title: Phycologia Volume: 58 Issue: 1 Pages: 63-69
  • DOI: 10.1080/00318884.2018.1517537
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Reinstatement of Phrix (Delesseriaceae, Rhodophyta) based on DNA sequence analyses and morpho-anatomical evidence (2018)
  • Author(s): Munoz, L. A., Wynne, M. J., Kamiya, M., West, J. A., Kupper, F. C., Ramirez, M. E. & Peters, A. F.
  • Journal Title: Phytotaxa Volume: 33 Issue: 3 Pages: 215-224
  • DOI: 10.11646/phytotaxa.334.3.2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Combining niche shift and population genetic analyses predicts rapid phenotypic evolution during invasion (2018)
  • Author(s): Sotka, E.E., Baumgardner, A.W., Bippus. P.M., Destombe, C., Duermit, E.A., Endo, H., Flanagan, B.A., Kamiya, M., Lees, L.E., Murren, C.J., Nakaoka, M., Shainker, S.J., Strand, A.E., Terada, R., Valero, M., Weinberger, F., Krueger-Hadfield, S.A.
  • Journal Title: Evolutionary Applications Volume: 11 Issue: 5 Pages: 781-793
  • DOI: 10.1111/eva.12592
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Non-monophyly of Bostrychia simpliciuscula (Ceramiales, Rhodophyta): multiple species with very similar morphologies, a revised taxonomy of cryptic species (2018)
  • Author(s): Zuccarello, G. C., West, J. A. & Kamiya, M.
  • Journal Title: Phycological Research Volume: 66 Issue: 2 Pages: 218-230
  • DOI: 10.1111/pre.12207
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] High species richness and genetic diversity of the genus Caloglossa (Delesseriaceae, Rhodophyta) in New Ireland, Papua New Guinea (2018)
  • Author(s): Kamiya, M., West, J. A. & Zuccarello, G. C.
  • Journal Title: Phycological Research Volume: 66 Issue: 3 Pages: 100-107
  • DOI: 10.1111/pre.12223
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Patterns and drivers of species diversity in the Indo-Pacific red seaweed Portieria (2018)
  • Author(s): Leliaert, F., Payo, D. A., Gurgel, C. F. D., Schils, T., Draisma, S. G., Saunders, G. W., Kamiya, M., Sherwood, A. R., Lin, S-M., Huisman, J. M., Le Gall, L., Anderson, R. J., Bolton, J. J., Mattio, L., Zubia, M., Spokes, T., Vieira, C., Payri, C. E., Coppejans, E., D’hondt, S., Verbruggen, H. & De Clerck, O.
  • Journal Title: Journal of Biogeography Volume: 2018 Issue: 10 Pages: 1-15
  • DOI: 10.1111/jbi.13410
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Marginal distribution and high heterozygosity of asexual Caloglossa vieillardii (Delesseriaceae, Rhodophyta) along the Australian coasts (2017)
  • Author(s): Kamiya Mitsunobu、Saba Erika、West John A.
  • Journal Title: Journal of Phycology Volume: 53 Issue: 6 Pages: 1283-1293
  • DOI: 10.1111/jpy.12580
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The Taurine Content of Japanese Seaweed (2017)
  • Author(s): Kawasaki, A., Ono, A., Mizuta, S., Kamiya, M., Takenaga, T., Murakami, S.
  • Journal Title: Advances in Experimental Medicine and Biology Volume: 975 Pages: 1105-1112
  • Peer Reviewed
• [Journal Article] Molecular identification of macroalgal fragments in gut contents of the sea urchin Hemicentrotus pulcherrimus. (2017)
  • Author(s): Nakano, D., Kamiya, M. & Tominaga, O.
  • Journal Title: Fisheries Science Volume: － Issue: 3 Pages: 425-432
  • DOI: 10.1007/s12562-017-1080-6
  • NAID: 40021193011
  • Peer Reviewed
• [Journal Article] Asexuality and the cryptic species problem. (2017)
  • Author(s): Dudgeon, S., Kubler, J. E., West, J. A., Kamiya, M. & Krueger-Hadfield, S. A.
  • Journal Title: Perspective in Phycology Volume: － Issue: 1 Pages: 47-59
  • DOI: 10.1127/pip/2017/0070
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular and morphological delineation of Caloglossa beccarii and related species (Delesseriaceae, Rhodophyta). (2016)
  • Author(s): Kamiya, M., West, J. A., Karsten, U., Ganesan, E. K.
  • Journal Title: Phycologia Volume: 55 Issue: 6 Pages: 640-649
  • DOI: 10.2216/16-19.1
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Segregative cell division and the cytoskeleton in two species of the genus Struvea (Cladophorales, Ulvophyceae, Chlorophyta). (2016)
  • Author(s): Okuda, K., Sekida, S., Hasebe, A., Iwabuchi, M., Kamiya, M., Hishinuma, T.
  • Journal Title: Phycological Research Volume: 64 Issue: 4 Pages: 219-229
  • DOI: 10.1111/pre.12139
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Diversity and oceanic distribution of the Parmales (Bolidophyceae), a picoplanktonic group closely related to diatoms. (2016)
  • Author(s): Kuwata, A., Ichinomiya, M., dos Santos, A. L., Gourvil, P., Yoshikawa, S., Kamiya, M., Ohki,K., Audic, S., de Vargas, C., Noël, M.-H. & Vaulot, D.
  • Journal Title: The ISME Journal Volume: 未定 Issue: 10 Pages: 2419-2434
  • DOI: 10.1038/ismej.2016.38
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mitotic spindle formation in Triparma laevis NIES-2565 (Parmales, Heterokontophyta). (2016)
  • Author(s): Yamada, K., Nagasato, C., Motmomura, T., Ichinomiya, M., Kuwata, A., Kamiya, M., Ohki, K. & Yoshikawa, S.
  • Journal Title: Protoplasma Volume: 未定 Issue: 1 Pages: 461-471
  • DOI: 10.1007/s00709-016-0967-x
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Diversity of phlorotannin profiles among sargassasacean species affecting variation and abundance of epiphytes. (2016)
  • Author(s): Nakajima, N., Sugimoto N., Ohki, K. & Kamiya, M.
  • Journal Title: European Journal of Phycology Volume: 未定
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Defense mechanisms of sargassacean algae against epiphytic red alga Neosiphonia harveyi. (2015)
  • Author(s): Nakajima, N., Ohki, K. & Kamiya, M.
  • Journal Title: Journal of Phycology Volume: 51 Issue: 4 Pages: 695-705
  • DOI: 10.1111/jpy.12311
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Caloglossa beccarii (Delesseriaceae, Rhodophyta) from freshwater rivers in Kerala, India, a critical new record (2015)
  • Author(s): West, J. A., Kamiya, M., Ganesan, E. K., Louiseaux-de Goër, S. & Jose L.
  • Journal Title: Algae Volume: 30 Issue: 3 Pages: 207-216
  • DOI: 10.4490/algae.2015.30.3.207
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 褐藻カヤモノリの単為生殖系統の起源について (2019)
  • Author(s): 星野 雅和・神谷 充伸・小亀 一弘
  • Organizer: 日本藻類学会第43回大会
• [Presentation] 隠蔽種を名付ける：日本産褐藻カヤモノリを6種に分ける (2019)
  • Author(s): 星野 雅和・田中 厚子・上井 進也・神谷 充伸・平岡 雅規・小亀 一弘
  • Organizer: 日本藻類学会第43回大会
• [Presentation] アオサ藻綱シオグサ目における細胞分裂様式の多様性と進化 (2019)
  • Author(s): 小山 知洋・奥田一雄・鈴木 秀和・田中 次郎・神谷充伸
  • Organizer: 日本藻類学会第43回大会
• [Presentation] アオサ藻綱シオグサ目における発生様式の多様性と進化 (2019)
  • Author(s): 小山 知洋・奥田一雄・鈴木 秀和・田中 次郎・神谷充伸
  • Organizer: 日本藻類学会第43回大会
• [Presentation] 宮城県志津川湾の海藻相 (2019)
  • Author(s): 福岡将之・阿部拓三・鈴木秀和・神谷充伸・田中次郎
  • Organizer: 日本藻類学会第43回大会
• [Presentation] 絶滅危惧海藻ホソエガサにおける至適培養条件の探索 (2018)
  • Author(s): 清水淳平・神谷充伸
  • Organizer: 日本植物学会第82回大会
• [Presentation] Ten years’ monitoring of subtidal algal beds of Hyogo Prefecture by the “Monitoring Sites 1000 Moba” Project (2018)
  • Author(s): Hiroshi Kawai, Mahiko Abe, Mitsunobu Kamiya, Akira Kurashima, Hiromori Shimabukuro, Noboru Murase, Goro Yoshida and Ryuta Terada
  • Organizer: The 12th International Conference on the Environmental Management of the Enclosed Coastal Seas
  • Int'l Joint Research
• [Presentation] 褐藻カヤモノリにおける単為発生生殖を行う雌優占集団：退化した有性的形質と新たに獲得した無性的形質 (2018)
  • Author(s): 星野雅和・神谷充伸・小亀一弘
  • Organizer: 日本藻類学会第2018年度大会
• [Presentation] 褐藻クロモズク（広義シオミドロ目）の分子系統解析と分類の再検討 (2018)
  • Author(s): 川井浩史・羽生田岳昭・柴田健介・神谷充伸
  • Organizer: 日本藻類学会第2018年度大会
• [Presentation] 日本各地の汽水環境におけるスジアオノリの遺伝子型と生殖細胞の走光性の違い (2017)
  • Author(s): 小川拓・神谷充伸
  • Organizer: 日本藻類学会第2017年度大会
  • Place of Presentation: 高知県高知
  • Year and Date: 2017-03-24
• [Presentation] 無性生殖型ササバアヤギヌの起源 (2017)
  • Author(s): 神谷充伸・佐波恵理加・John A. West.
  • Organizer: 日本藻類学会第2017年度大会
  • Place of Presentation: 高知県高知
  • Year and Date: 2017-03-24
• [Presentation] Rapid evolution of stress tolerance facilitates the invasion of an ecosystem engineer. (2017)
  • Author(s): E.E. Sotka; A.W. Baumgardner; P. Bippus; C. Destombe; E. Duermit; H. Endo; B.A. Flanagan; M. Kamiya; L. Lees; C.J. Murren; M. Nakaoka; S. Shainker; A.E. Strand; R. Terada; M. Valero; F. Weinberger; S.A. Krueger-Hadfield.
  • Organizer: Benthic Ecology Meeting
  • Int'l Joint Research
• [Presentation] Non-monophyly of Bostrychia simpliciuscula (Ceramiales, Rhodophyta): multiple species with very similar morphologies, a revised taxonomy of cryptic species. (2017)
  • Author(s): Zuccarello, G. C., West, J. A. & Kamiya, M.
  • Organizer: 11th International Phycological Congress
  • Int'l Joint Research / Invited
• [Presentation] Marginal distribution and high heterozygosity of asexual Caloglossa vieillardii (Delesseriaceae, Rhodophyta) along the Australian coasts. (2017)
  • Author(s): Kamiya, M., Saba, E. & West, J. A.
  • Organizer: 11th International Phycological Congress
  • Int'l Joint Research
• [Presentation] Comparison of genetic diversity and distribution patterns among marine and freshwater Cladophora species in Japan (2017)
  • Author(s): Kamiya, M., Niwa, K. & Hayakawa, Y.
  • Organizer: 8th Asia Pacific Phycological Forum
  • Int'l Joint Research
• [Presentation] フノリのマウスにおける血糖上昇抑制作用 (2017)
  • Author(s): 川﨑安都紗・小野鮎子・木村公一・水田尚志・神谷充伸・大脇豊・大脇豊弘・伊藤 崇志・村上茂
  • Organizer: 第10回北陸合同シンポジウム
• [Presentation] 若狭湾産海藻類における生理活性成分の研究 ～抗アレルギー成分について～ (2017)
  • Author(s): 五十嵐彩香・細井公富・水田尚志・神谷充伸・横山芳博
  • Organizer: 平成29年度日本水産学会中部支部大会
• [Presentation] 近縁な海産・淡水産シオグサの遺伝的多様性と分布パターンの比較 (2016)
  • Author(s): 丹羽一夫・神谷充伸
  • Organizer: 平成28年度水産学会中部支部会大会
  • Place of Presentation: 福井県小浜
  • Year and Date: 2016-12-03
• [Presentation] 福井県産海藻中のタウリン含量の分析 ～海藻を活用した機能性食品の開発～ (2016)
  • Author(s): 川崎安都紗・小野鮎子・水田尚志・神谷充伸・大脇豊・大脇豊弘・村上茂.
  • Organizer: 北陸合同バイオシンポジウム2016年度大会
  • Place of Presentation: 福井県あわら
  • Year and Date: 2016-11-04
• [Presentation] 若狭湾産海藻類に含まれる生理活性物質の探索1 ～抗アレルギー成分～ (2016)
  • Author(s): 五十嵐彩香・細井公富・水田尚志・神谷充伸・横山芳博
  • Organizer: 北陸合同バイオシンポジウム2016年度大会
  • Place of Presentation: 福井県あわら
  • Year and Date: 2016-11-04
• [Presentation] 若狭湾産海藻類に含まれる生理活性物質の探索2 ～抗肥満性成分～ (2016)
  • Author(s): 及川聡美・細井公富・水田尚志・神谷充伸・横山芳博
  • Organizer: 北陸合同バイオシンポジウム2016年度大会
  • Place of Presentation: 福井県あわら
  • Year and Date: 2016-11-04
• [Presentation] その他の海藻の現状と未来. (2016)
  • Author(s): 神谷充伸
  • Organizer: 北陸合同バイオシンポジウム2016年度大会
  • Place of Presentation: 北海道函館
  • Year and Date: 2016-05-28
  • Invited
• [Presentation] Taurine content in Japanese seaweed. (2016)
  • Author(s): Kawasaki, A., Ono, A., Mizuta, S., Kamiya, M. & Murakami, S.
  • Organizer: International Taurine Meeting.
  • Place of Presentation: Soul, South Korea
  • Year and Date: 2016-05-24
  • Int'l Joint Research
• [Presentation] 緑藻エビヤドリモ属Cladogoniumにおける複子嚢タイプの発見と新目の提唱 (2016)
  • Author(s): 芹澤(松山)和世・今井 正・大貫貴清・中曽雅之・小川 拓・丹羽一夫・神谷充伸・芹澤如比古
  • Organizer: 日本藻類学会2016年大会
  • Place of Presentation: 東京歯科大学（東京）
  • Year and Date: 2016-03-19
• [Presentation] 無性型スジアオノリ（Ulva prolifera）の遺伝子型間における淡水耐性の違い (2016)
  • Author(s): 小川拓・神谷充伸
  • Organizer: 日本藻類学会2016年大会
  • Place of Presentation: 東京歯科大学（東京）
  • Year and Date: 2016-03-19
• [Presentation] 海藻中タウリン含量の分析 (2016)
  • Author(s): 川崎安都紗・小野鮎子・水田尚志・神谷充伸・村上茂
  • Organizer: 第2回国際タウリン研究会日本部会
  • Place of Presentation: 福井県立大学（福井）
  • Year and Date: 2016-03-05
• [Presentation] 淡水エビに寄生するエビヤドリモ属Cladogonium藻類には複数種いるのか？ (2015)
  • Author(s): 芹澤如比古・今井 正・大貫貴清・中曽雅之・小川拓・丹羽一夫・神谷充伸・芹澤（松山）和世.
  • Organizer: 日本陸水学会甲信越支部会
  • Place of Presentation: 月岡ニューホテル冠月（新潟）
  • Year and Date: 2015-11-28
• [Presentation] DNAバーコーディング法を用いたバフンウニの摂餌海藻の同定 (2015)
  • Author(s): 仲野大地・神谷充伸・富永修
  • Organizer: 日本水産学会中部支部会
  • Place of Presentation: 東海大学（静岡）
  • Year and Date: 2015-11-21
• [Presentation] パルマ藻Triparma laevisの珪酸質殻構築過程. (2015)
  • Author(s): 山田和正・桂大貴・佐藤晋也・神谷充伸・一宮睦雄・桑田晃・長里千香子・本村泰三・大城香・吉川伸哉
  • Organizer: 日本植物学会2015年度大会
  • Place of Presentation: 新潟コンベンションセンター（新潟）
  • Year and Date: 2015-09-06
• [Presentation] 多核緑藻サイノメアミハの分割細胞分裂と細胞骨格の動態 (2015)
  • Author(s): 岩淵美紗・神谷充伸・関田諭子・奥田 一雄
  • Organizer: 日本植物学会2015年度大会
  • Place of Presentation: 新潟コンベンションセンター（新潟）
  • Year and Date: 2015-09-06
• [Presentation] Ultrastructural analysis of mitosis in Triparma laevis (Parmales, Heterokontophyta). (2015)
  • Author(s): Kazumasa Yamada, Chikako Nagasato1, Taizo Motomura, Mutsuo Ichinomiya, Akira Kuwata, Mitsunobu Kamiya, Kaori Ohki and Shinya Yoshikawa
  • Organizer: European Phycological Congress
  • Place of Presentation: Hotel Novotel London West (London, UK)
  • Year and Date: 2015-08-23
  • Int'l Joint Research
• [Presentation] Growth and cell wall morphogenesis of Triparma laevis (Parmales) under silicon-limitation. (2015)
  • Author(s): Kazumasa Yamada, Mutsuo Ichinomiya, Akira Kuwata, Mitsunobu Kamiya, Kaori Ohki, Shinya Yoshikawa.
  • Organizer: Molecular Life of Diatoms
  • Place of Presentation: University of Washington (Seattle, USA)
  • Year and Date: 2015-07-07
  • Int'l Joint Research
• [Presentation] 汽水域に生育するスジアオノリの遺伝的・生殖的多様性と分布特性. (2015)
  • Author(s): 小川拓・吉川伸哉・神谷充伸
  • Organizer: 北陸植物学会2015年度大会
  • Place of Presentation: 金沢大学（金沢）
  • Year and Date: 2015-06-21
• [Presentation] Cyanothece sp. Viet Nam 01株の金属耐性と金属吸着性の評価. (2015)
  • Author(s): 大山聡士・牧しづか・神谷充伸・大城香・吉川伸哉
  • Organizer: 北陸植物学会2015年度大会
  • Place of Presentation: 金沢大学（金沢）
  • Year and Date: 2015-06-21
• [Book] Syllabus of Plant Families: Part 2/2. Photoautotrophic eukaryotic Algae Rhodophyta. (2017)
  • Author(s): Kamiya, M., Lindstrom, S. C., Nakayama, T., Yokoyama, A., Lin, S.-M., Guiry, M. D., Gurgel, C. F. D., Huisman, J. M., Kitayama, T., Suzuki, M., Cho, T. O. & Frey, W.
  • Total Pages: 171
  • Publisher: Borntraeger Science Publishers
  • ISBN: 9783443010942
• [Book] Syllabus of Plant Families: Part 2/2. Photoautotrophic eukaryotic Algae Rhodophyta (2017)
  • Author(s): 8.Kamiya, M., Lindstrom, S. C., Nakayama, T., Yokoyama, A., Lin, S.-M., Guiry, M. D., Gurgel, C. F. D., Huisman, J. M., Kitayama, T., Suzuki, M., Cho, T. O. & Frey, W.
  • Total Pages: 171
  • Publisher: Borntraeger Science Publishers
• [Book] 海藻の疑問50（みんなが知りたいシリーズ） (2016)
  • Author(s): 日本藻類学会 編集
  • Total Pages: 168
  • Publisher: 成山堂書店