Molecular characterization of gliadins in aneuploid lines of Chinese Spring wheat

• Project/Area Number: 15K07261
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Science in genetics and breeding
• Research Institution: Yokohama City University
• Principal Investigator: Kawaura Kanako 横浜市立大学, 木原生物学研究所, 准教授 (60381935)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: パンコムギ / 種子貯蔵タンパク質 / 小麦アレルギー / グリアジン / ゲノム / コムギ / アレルギー / 遺伝子発現制御

Outline of Final Research Achievements

Seed storage proteins are encoded by multigenes in bread wheat. Gliadins are encoded by extremely large multigene family and some kinds of them cause allergy symptoms or celiac disease (CD) in human. To characterize distinct gliadins, two-dimensional electrophoresis was conducted using gliadins extracted from aneuploid lines of Chinese Spring wheat. Gliadins were separated into 90 spots and these spots were assigned to the chromosomal loci. Among lines, we found lines in which specific gliadins harboring CD epitopes were absent. Genomic PCR showed that these lines carry deletion of the chromosome segment containing the gliadin locus on the 6D chromosome. In addition, an antibody, which reacts the specific gliadins harboring CD epitopes, were designed and constructed. These liens and the antibody could be useful resource in breeding programs for reduction of CD immunotoxicity.

Academic Significance and Societal Importance of the Research Achievements

多重遺伝子にコードされるためコピー数が非常に多く、小麦アレルギーの原因ともなるコムギ種子貯蔵タンパク質の特徴づけを行った。その中で、アレルギーの原因となる特定の種子貯蔵タンパク質が欠失しているコムギ系統を見出した。これらから、低アレルゲン小麦作出に向けた基盤となる情報を示した。

Research Products

• [Journal Article] Molecular characterization of gliadins of Chinese Spring wheat in relation to celiac disease elicitors (2018)
  • Author(s): Kanako Kawaura, Mayuko Miura, Yoko Kamei, Tatsuya M. Ikeda, Yasunari Ogihara
  • Journal Title: Genes & Genetic Systems Volume: 93 Issue: 1 Pages: 9-20
  • DOI: 10.1266/ggs.17-00034
  • NAID: 130007404293
  • ISSN: 1341-7568, 1880-5779
  • Year and Date: 2018-02-01
  • Peer Reviewed
• [Journal Article] Contribution of α-gliadin alleles to the extensibility of flour dough in Japanese wheat cultivars (2018)
  • Author(s): Satoshi Noma, Katsuyuki Hayakawa, Chikako Abe, Sayaka Suzuki, Kanako Kawaura
  • Journal Title: Journal of Cereal Science Volume: 86 Pages: 15-21
  • DOI: 10.1016/j.jcs.2018.12.017
  • Peer Reviewed
• [Presentation] Characterization of gliadins using aneuploids of Chinese Spring wheat reveals genome-specific gliadin regulation (2017)
  • Author(s): Kanako Kawaura, Mayuko Miura, Yoko Kamei, Tatsuya M Ikeda, Yasunari Ogihara
  • Organizer: 13th International Wheat Genetics Symposium
  • Place of Presentation: トゥルン（オーストリア）
  • Year and Date: 2017-04-23
  • Int'l Joint Research
• [Presentation] パンコムギChinese Springの特定のα-グリアジンを抑制する因子の解析 (2017)
  • Author(s): 鈴木彩夏、亀井葉子、三浦麻友子、荻原保成、川浦香奈子
  • Organizer: 日本育種学会第131回講演会
  • Place of Presentation: 名古屋大学（愛知県名古屋市）
  • Year and Date: 2017-03-29
• [Presentation] Characterization of gliadins using aneuploids of Chinese Spring wheat reveals genome-specific gliadin regulation (2017)
  • Author(s): 17.Kanako Kawaura, Mayuko Miura, Yoko Kamei, Tatsuya M Ikeda, Yasunari Ogihara
  • Organizer: 13th International Wheat Genetics Symposium
  • Int'l Joint Research
• [Presentation] Characterization of gliadins using aneuploid lines in Chinese Spring wheat (2016)
  • Author(s): Kawaura K, Miura M, Ikeda TM, Ogihara Y.
  • Organizer: Plant and Animal Genome XXIV
  • Place of Presentation: サンディエゴ（アメリカ）
  • Year and Date: 2016-01-09
  • Int'l Joint Research