Mechanism of temperature-dependent flower malformation in carnations

• Project/Area Number: 15K07285
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Horticultural science
• Research Institution: Utsunomiya University
• Principal Investigator: Yamane Kenji 宇都宮大学, 農学部, 教授 (60240066)
• Co-Investigator(Kenkyū-buntansha): 黒倉 健 宇都宮大学, 農学部, 講師 (10650898)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 奇形花 / 変異 / 温度依存 / 遺伝子発現 / 花芽分化 / 花芽分化ステージ / 温度 / 花 / 形態異常

Outline of Final Research Achievements

Temperature regimes that cause malformed flowers were examined by using mutants of the potted carnation ‘Cherie’. Plants of normal (WT) and malformed (mlf) lines were grown under several temperature regimes. This flower malformation was reversible depending on the temperature regime. mlf plants produced 92.2% of malformed flowers under constant 15°C, whereas flower malformation was significantly lower under 20°C and 25/20°C. Morphological differences between WT and mlf after sepal formation. RNA-seq revealed that HSC70，Wushcel and LOG3 genes were upregulated in flower buds of mlf plants at 15°C. Mechanism of this malformation was discussed.

Research Products

• [Journal Article] Temperature-dependent Flower Malformation in Carnations (<i>Dianthus caryophyllus</i> L.) (2018)
  • Author(s): Yamane, K., K. Sumida, Y. Terui, N. Kojima, C. Burana and T. Kurokura.
  • Journal Title: The Horticulture Journal Volume: 87 Issue: 3 Pages: 406-412
  • DOI: 10.2503/hortj.OKD-151
  • NAID: 130007420621
  • ISSN: 2189-0102, 2189-0110
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] カーネーションにおける温度依存性の異常花発生 (2017)
  • Author(s): 山根健治・照井友梨・住田北郎・Burana Chairat・黒倉 健
  • Organizer: 園芸学会平成28年度春季大会
• [Presentation] カーネーションにおける温度依存性の異常花発生と花芽分化ステージ (2017)
  • Author(s): 山根健治・照井友梨・小島渚沙・黒倉 健
  • Organizer: 園芸学会平成28年度秋季大会
• [Presentation] カーネーションにおける温度依存性の異常花についての分子生物学的解析 (2017)
  • Author(s): 山根健治・黒倉 健・鈴木智大・小島渚沙・ウォララッド カンジャンナ・石井一夫
  • Organizer: 園芸学会平成29年度秋季大会
• [Presentation] カーネーションにおける温度依存性の異常花発生. (2016)
  • Author(s): 山根健治・照井友梨・住田北郎・Burana Chairat・黒倉 健.
  • Organizer: 園芸学会平成28年度春季大会． 園芸学研究 15(別1)：232.
  • Place of Presentation: 東京農業大学厚木キャンパス
  • Year and Date: 2016-03-26
• [Presentation] カーネーションにおける温度依存性の異常花発生と花芽分化ステージ． (2016)
  • Author(s): 山根健治・照井友梨・小島渚沙・黒倉 健.
  • Organizer: 園芸学会平成28年度秋季大会
  • Place of Presentation: 名城大学