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Analysis of the role of brassinosteroid during strawberry fruit development

Research Project

Project/Area Number 15K07296
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Horticultural science
Research InstitutionYokohama City University

Principal Investigator

Nakamura Ayako  横浜市立大学, 木原生物学研究所, 助教 (40585858)

Co-Investigator(Kenkyū-buntansha) 筧 雄介  横浜市立大学, 木原生物学研究所, 特任助教 (50636727)
Research Collaborator SHIMADA Yukihisa  
Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords二倍体イチゴ / 植物ホルモン / 果実発達 / ブラシノステロイド / 生合成阻害剤 / 矮性 / 雌性不稔
Outline of Final Research Achievements

The aim of this study was to reveal the role of brassinosteroid (BR) during fruit set and development. The fact that BR treated emasculated flower did not start developing its fruit suggesting BR does not have ability to induce parthenocarpic fruit development. The length of berries produced from the strawberry treated with BR biosynthesis inhibitor was shorter than that from mock treated ones. While, the BR treated strawberries produce longer berries. The difference in berry length was visible after white berry stage. These results suggest that BR regulated the length of berries during later fruit development. In BR biosynthesis inhibitor-treated strawberry, female fertility was much lower than in the mock treated plants. All the phenotypes affeced by BR biosynthesis inhibitor treatment was recovered by BR treatment suggesting the phenotypes was caused by the lack of BR.

Academic Significance and Societal Importance of the Research Achievements

今回の研究結果により、BRは単為結果を誘導する能力はないことが示唆されたが、遺伝子の発現と形態の解析から着果前後の発達に重要であることが初めて示された。今後の研究によりBR欠損により雌性稔性の低下する原因が特定できれば、着果不良が原因によってはBR投与により改善できる可能性が示された。また、BRは果実発達後期の長径方向の発達にも関与することが示唆され、さらに、BRにより通常の受粉により発達した果実よりも肥大が促進されたことから、大粒イチゴを生産する際にBRが有用である可能性を示した。今後は我々が通常食している8倍体品種における応用研究が期待される。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (2 results)

All 2018 2017

All Presentation (2 results)

  • [Presentation] 二倍体イチゴの果実発達におけるブラシノステロイドの作用解析2018

    • Author(s)
      石井ひかり、嶋田幸久、中村郁子
    • Organizer
      園芸学会平成30年秋季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ブラシノステロイド生合成阻害を用いた二倍体イチゴの果実発達機構の解析2017

    • Author(s)
      石井 ひかり 中村 郁子 嶋田 幸久
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Research-status Report

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Published: 2015-04-16   Modified: 2020-03-30  

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