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Analysis of the environmental regulation of the secondary metabolism in grape berries

Research Project

Project/Area Number 15K07306
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Horticultural science
Research InstitutionNational Research Institute of Brewing

Principal Investigator

Kazuya Koyama  独立行政法人酒類総合研究所, 研究部門, 主任研究員 (30416424)

Project Period (FY) 2015-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords二次代謝 / 環境応答 / フェノール化合物 / 香気成分前駆体 / 遺伝子発現解析 / ブドウ / 転写制御因子 / イソプレノイド / 遺伝子発現 / 色素芳香成分 / 機能性成分 / ゲノム科学
Outline of Final Research Achievements

To elucidate the mechanisms of environmental regulation of the secondary metabolism in grape berries, first, we set up the analytical method to analyze comprehensively the phenolics and the volatile compounds, both free and bound forms. By the method, we could detect the significant modulation of the composition of the secondary metabolites among the varieties as well as under different environmental conditions, such as light environment around the bunches. Genechip analysis screened a candidate bZIP transcription factor whose gene expression profile was coordinate to those of the phenolics and terpenoids ubder different environment and developmental stages. The functional analysis of this bZIP transcription factor revealed the regulation of the phenolic metabolism. For the regulation of the secondary metabolism by this factor, co-factor was considered to be necessary.

Academic Significance and Societal Importance of the Research Achievements

開発した二次代謝成分の網羅的解析法(香気成分の前駆体の解析を含む)は,栽培法や気象の影響解析などの様々な課題に適用することによって,醸造用ブドウの品質評価が可能であり,実用的な面にも今後貢献できると期待される。また,ブドウにおける二次代謝成分の制御機構に関する知見は限られており,学術的にも新規性は高いと考えられる。

Report

(6 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (6 results)

All 2019 2017 2016 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 1 results) Presentation (3 results) (of which Int'l Joint Research: 2 results) Remarks (1 results)

  • [Journal Article] The potential aroma and flavor compounds in Vitis sp. cv. Koshu and V. vinifera L. cv. Chardonnay under different environmental conditions2019

    • Author(s)
      Sharon Marie Bahena-Garrido, Tomoko Ohama, Yuka Suehiro, Yuko Hata, Atsuko Isogai, Kazuhiro Iwaashita, Nami Goto-Yamamoto and Kazuya Koyama*
    • Journal Title

      Journal of the science of food and agriculture

      Volume: 99 Pages: 1926-1937

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Polyphenolic diversity and characterization in the red-purple berries of East Asian wild Vitis species2017

    • Author(s)
      Kazuya Koyama*, Hiroshi Kamigakiuchi, Kazuhiro iwashita, Ryosuke Mochioka, Nami Goto-Yamamoto
    • Journal Title

      Phytochemistry

      Volume: 134 Pages: 78-86

    • DOI

      10.1016/j.phytochem.2016.10.003

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] The effect of phytohormones and shading on the aroma and flavor characteristics in Koshu and Chardonnay varieties2017

    • Author(s)
      Garrido Sharon-Marie, Tomoko Ohama, Yuka Suehiro, Yuko Hata, Atsuko Isogai, Kazuhiro Iwashita, Nami Goto-Yamamoto, Kazuya Koyama
    • Organizer
      ISHS 13th international symposium on plant bioregulators in fruit production
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 各種栽培処理が‘甲州’‘シャルドネ’果実のフェノール化合物及び香気成分組成へ及ぼす影響2016

    • Author(s)
      小山和哉*、大浜友子、末廣優加、清水英明、磯谷敦子、江角智也、後藤(山本)奈美
    • Organizer
      日本ブドウ・ワイン学会2016大会
    • Place of Presentation
      山梨大学甲府キャンパス
    • Year and Date
      2016-11-26
    • Related Report
      2016 Research-status Report
  • [Presentation] Polyphenolic diversity and characterization in the red-purple berries of East Asian wild Vitis species2016

    • Author(s)
      Kazuya Koyama*, Hiroshi. Kamigakiuchi, Kazuhiro Iwashita, Ryosuke Mochioka, and Nami Goto-Yamamoto
    • Organizer
      10th international symposium of grapevine physiology & biotechnology
    • Place of Presentation
      Verona, Italy
    • Year and Date
      2016-06-13
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Remarks] 最近の研究成果

    • URL

      http://www.nrib.go.jp/data/seika/NewSeika.htm

    • Related Report
      2016 Research-status Report

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Published: 2015-04-16   Modified: 2021-02-19  

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