Waking up cryptic secondary metabolisms in actinomycetes by a butenolide-type Streptomyces hormone
| Project/Area Number |
15K07358
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Osaka University |
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Principal Investigator |
Kitani Shigeru 大阪大学, 生物工学国際交流センター, 准教授 (10379117)
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| Co-Investigator(Renkei-kenkyūsha) |
ARAI Masayoshi 大阪大学, 大学院薬学研究科, 特任教授 (80311231)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 抗生物質生産 / 放線菌 / ブテノライド型シグナル / 潜在二次代謝 |
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| Outline of Final Research Achievements |
Streptomyces hormones induce the production of secondary metabolites by binding to the cognate receptors in actinomycetes. In this study, I aimed to awaken cryptic useful secondary metabolites through the manipulation of signaling cascades governed by Streptomyces hormones, and to identify actinomycetes strains in which butenolide-type Streptomyces hormones control secondary metabolism. I demonstrated that gene disruption of Streptomyces-hormone receptor activates the production of phthoxazolin A in Streptomyces avermitilis, and that phthoxazolin A biosynthetic genes are encoded on an extra genomic region that is not found in the public database. Furthermore, identification of butenolide-type Streptomyces hormone-like compounds from Streptomyces albus J1074 led to structure-activity relationship for butenolide-type Streptomyces hormones, as well as chemical communication between different streptomycetes via Streptomyces hormones.
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Report
(4 results)
Research Products
(27 results)
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[Journal Article] Discovery of a new diol-containing polyketide by heterologous expression of a silent biosynthetic gene cluster from Streptomyces lavendulae FRI-5.2018
Author(s)
2.Pait, IGU., Kitani, S., Roslan, FW., Ulanova, D., Arai, M., Ikeda, H., Nihira, T.
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Journal Title
J. Ind. Microbio. Biotechnol.
Volume: 45
Issue: 2
Pages: 77-87
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Identification and characterization of lbpA, an indigoidine biosynthetic gene in the γ-butyrolactone signaling system of Streptomyces lavendulae FRI-5.2017
Author(s)
5.Pait, IGU., Kitani, S., Kumiawan, YN., Asa, M., Iwau, T., Ikeda, H. Nihira, T.
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Journal Title
J. Biosci. Bioeng.
Volume: 124
Issue: 4
Pages: 369-375
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Characterization of AvaR1, a butenolide-autoregulator receptor for biosynthesis of a Streptomyces hormone in Streptomyces avermitilis2016
Author(s)
Sultan, SP., Kitani, S., Miyamoto, KT., Iguchi, H., Atago, T., Ikeda, H., Nihira, T.
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Journal Title
Appl. Microbiol. Biotechnol.
Volume: 100
Issue: 22
Pages: 9581-9591
DOI
Related Report
Peer Reviewed / Open Access
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