Mechanism of link between energy homeostasis and onset of differentiation in Streptomyces

• Project/Area Number: 15K07370
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Nihon University
• Principal Investigator: UEDA Kenji 日本大学, 生物資源科学部, 教授 (00277401)
• Co-Investigator(Kenkyū-buntansha): 高野 英晃 日本大学, 生物資源科学部, 准教授 (50385994) ; 西山 辰也 日本大学, 生物資源科学部, 助手 (10759541)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: Streptomyces / energy metabolism / cell differentiation / secondary metabolism / homeostasis / transcription / regulation / antibiotics / エネルギー代謝 / 遺伝制御 / 恒常性 / 二次代謝 / 形態分化

Outline of Final Research Achievements

Antibiotic production and morphological differentiation in Streptomyces is stimulated by a high concentration of copper ion. Here, we revealed that the stimulation is based on the energy homeostatic mechanisms including the following systems: (1) negative regulation of ATP synthase operon by a transcriptional regulator Rex, and (2) positive regulation of succinate dehydrogenase operon by a specific LysR-type transcriptional regulator. Thus controlled intracellular energetic state may serve as a signal for the onset of development.

Research Products

• [Journal Article] Antibiotics in microbial coculture (2017)
  • Author(s): Kenji Ueda and Teruhiko Beppu
  • Journal Title: J Antibiot Volume: 70 Issue: 4 Pages: 361-365
  • DOI: 10.1038/ja.2016.127
  • Peer Reviewed
• [Journal Article] Developmental defect of cytochrome oxidase mutants of Streptomyces coelicolor A3(2) (2016)
  • Author(s): Fujimoto M, Chijiwa M, Nishiyama T, Takano H, Ueda K
  • Journal Title: Microbiologoy Volume: 162 Issue: 8 Pages: 1446-1455
  • DOI: 10.1099/mic.0.000332
  • Peer Reviewed
• [Journal Article] Streptomyces metabolites in divergent microbial interactions. (2016)
  • Author(s): Takano H, Nishiyama T, Amano S, Beppu T, Kobayashi M, Ueda K
  • Journal Title: J Ind Microbiol Biotechnol Volume: 43 Issue: 2-3 Pages: 143-148
  • DOI: 10.1007/s10295-015-1680-z
  • Peer Reviewed / Open Access
• [Presentation] 放線菌Streptomyces griseusが保有する呼吸鎖複合体IIの銅イオン濃度特異的な転写制御 (2018)
  • Author(s): 高原薫平、上田賢志、髙野英晃
  • Organizer: 日本農芸化学会2018年度大会
• [Presentation] Newly discovered endogenous signaling routes in Streptomyces: the role of ATP and nitric oxide (2016)
  • Author(s): Kenji Ueda
  • Organizer: The 13th International Symposium on the Genetics of Industrial Microorganisms
  • Place of Presentation: Wuhan, China
  • Year and Date: 2016-10-17
  • Int'l Joint Research / Invited
• [Presentation] 放線菌におけるエネルギー代謝と分化・抗生物質生産の連携に関する研究 (2016)
  • Author(s): 千々和 雅人、高野 英晃、上田 賢志
  • Organizer: 日本農芸化学会平成２８年度大会
  • Place of Presentation: 札幌コンベンションセンター(北海道札幌市)
  • Year and Date: 2016-03-30