Estimation of Chemical Stractures of Soybean Saponins by LC-MS/MS Analysis
| Project/Area Number |
15K07451
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Iwate University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 大豆 / サポニン / 化学構造解析 / 遺伝解析 / プロファイル解析 / LC-PDA/MS/MS / 大豆加工食品 / NMR |
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| Outline of Final Research Achievements |
Saponin contained in soybean seeds is a generic term for a various molecular species. It has been reported that the health and taste properties of soybean saponins differ greatly due to the difference in chemical structure, but there remained many unclear points and re-evaluation was required. On the other hand, it has been clarified that the soybean seed saponins are biosynthesized by the combination of co-dominant, dominant, and recessive acting multiple genes.
In this study, to estimate the chemical structure of various soybean saponins which could not be elucidated for reasons such as trace, instability, or separation difficulty, we developed a new method (profile analysis) which is comprised of the comprehensive judgment with a combination of instrumental analysis and genetic analysis. By using this new method, it is thought that the chemical structures of various soybean saponins which changed during the food processing can be presumed in the future.
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Report
(4 results)
Research Products
(43 results)
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[Journal Article] Metabolic switching of astringent and beneficial triterpenoid saponins in soybean is achieved by a loss-of-function mutation in Cytochrome P450 72A692017
Author(s)
Yano, Ryoichi; Takagi, Kyoko; Takada, Yoshitake; Mukaiyama, Kyosuke; Tsukamoto, Chigen; Sayama, Takashi; Kaga, Akito; Sawai, Satoru; Ohyama, Kiyoshi; Saito, Kazuki; Ishimoto, Masao
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Journal Title
The Plant Journal
Volume: 89
Issue: 3
Pages: 527-539
DOI
Related Report
Peer Reviewed
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[Presentation] Identification of mutants in the soyasaponin biosynthetic pathway using a high-density soybean mutant library: an indispensable resource for functional genomics2017
Author(s)
Krishnamurthy Panneerselvam, Yukiko Fujisawa, Hanako Abe, Urara Ohashi, Kentaro Yamane, Kyosuke Mukaiyama, Chigen Tsukamoto, Akito Kaga, Toyoaki Anai, and Masao Ishimoto
Organizer
Plant & Animal Genome XXV
Place of Presentation
San Diego, California, USA
Year and Date
2017-01-14
Related Report
Int'l Joint Research
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[Presentation] LC-PDA/MS/MS解析で検出された変異誘発剤EMS処理大豆の種子サポニン組成変異2015
Author(s)
大橋 麗, 阿部華子, 板橋芽依, Jeong Hwa Kim, 米田咲子, 加賀秋人, 穴井豊昭, Jong Tae Song, Jeong Dong Lee, Gyuhwa Chung, 石本政男, 塚本知玄
Organizer
日本育種学会第128回講演会
Place of Presentation
新潟大学（新潟県新潟市）
Year and Date
2015-09-10
Related Report
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