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Enantioseparation of aldohexoses and aldopentoses with L-tryptophanamide for detecting L-monosaccharides by reversed phase HPLC using HPLC

Research Project

Project/Area Number 15K07458
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Food science
Research InstitutionTokai University

Principal Investigator

Kodama Shuji  東海大学, 理学部, 教授 (70360807)

Co-Investigator(Kenkyū-buntansha) 多賀 淳  近畿大学, 薬学部, 准教授 (20247951)
會澤 宣一  富山大学, 大学院理工学研究部(工学), 教授 (60231099)
山本 敦  中部大学, 応用生物学部, 教授 (60360806)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords光学異性体 / 単糖 / 高速液体クロマトグラフィー / 野菜 / HPLC / 誘導体化 / ジアステレオマー / 逆相HPLC
Outline of Final Research Achievements

D-Monosaccharides are distributed widely in nature, but it is not clarified whether L-monosaccharides, such as L-galactose, present in nature. We developed a novel reversed phase high-performance liquid chromatography for simultaneous enantioseparation of aldohexoses and aldopentoses derivatised with L-tryptophanamide. Using the above method, we tried to detect L-galactose in 8 vegetables. As a result, a small peak in only komatsuna sample was detected at the retention time of L-galactose. It is necessary to identify whether the peak is L-galactose in future.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (6 results)

All 2017 2016 2015 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (3 results) Remarks (2 results)

  • [Journal Article] Simultaneous Enantioseparation of Aldohexoses and Aldopentoses Derivatized with L-Tryptophanamide by Reversed Phase HPLC Using Butylboronic Acid as a Complexation Reagent of Monosaccharides2015

    • Author(s)
      M. Shou, H. Terashima, S. Aizawa, A. Taga, A. Yamamoto, S. Kodama
    • Journal Title

      Chirality

      Volume: 27 Issue: 7 Pages: 417-421

    • DOI

      10.1002/chir.22456

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] 野菜中のガラクトース光学異性体分析2017

    • Author(s)
      岡田眞希子、山本敦、多賀淳、會澤宣一、寺島弘之、小玉修嗣
    • Organizer
      日本分析化学会第66年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 単糖類の光学異性体分析2016

    • Author(s)
      小玉 修嗣
    • Organizer
      日本分析化学会第65年会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2016-09-14
    • Related Report
      2016 Research-status Report
  • [Presentation] L-アミノ酸誘導体化単糖の光学異性体分離2015

    • Author(s)
      翔美怜,寺島弘之,會澤宣一,多賀淳,山本敦,小玉修嗣
    • Organizer
      日本分析化学会第64年会
    • Place of Presentation
      九州大学伊都キャンパス
    • Year and Date
      2015-09-09
    • Related Report
      2015 Research-status Report
  • [Remarks] 東海大学理学部化学科 小玉研究室

    • URL

      http://kodamaken.wixsite.com/kodama

    • Related Report
      2017 Annual Research Report
  • [Remarks] 東海大学理学部化学科 小玉研究室

    • URL

      http://kodamaken.wixsite.com/kodama

    • Related Report
      2016 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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