Enantioseparation of aldohexoses and aldopentoses with L-tryptophanamide for detecting L-monosaccharides by reversed phase HPLC using HPLC
| Project/Area Number |
15K07458
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Tokai University |
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Principal Investigator |
Kodama Shuji 東海大学, 理学部, 教授 (70360807)
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| Co-Investigator(Kenkyū-buntansha) |
多賀 淳 近畿大学, 薬学部, 准教授 (20247951)
會澤 宣一 富山大学, 大学院理工学研究部(工学), 教授 (60231099)
山本 敦 中部大学, 応用生物学部, 教授 (60360806)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 光学異性体 / 単糖 / 高速液体クロマトグラフィー / 野菜 / HPLC / 誘導体化 / ジアステレオマー / 逆相HPLC |
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| Outline of Final Research Achievements |
D-Monosaccharides are distributed widely in nature, but it is not clarified whether L-monosaccharides, such as L-galactose, present in nature. We developed a novel reversed phase high-performance liquid chromatography for simultaneous enantioseparation of aldohexoses and aldopentoses derivatised with L-tryptophanamide. Using the above method, we tried to detect L-galactose in 8 vegetables. As a result, a small peak in only komatsuna sample was detected at the retention time of L-galactose. It is necessary to identify whether the peak is L-galactose in future.
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Report
(4 results)
Research Products
(6 results)
