| Project/Area Number |
15K07490
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Forest science
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| Research Institution | Forest Research and Management Organization |
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Principal Investigator |
Tahara Ko 国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (70445740)
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| Co-Investigator(Kenkyū-buntansha) |
西口 満 国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (80353796)
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| Research Collaborator |
MILKOWSKI Carsten マルティン・ルター大学, ハレ・ヴィッテンベルク
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 加水分解性タンニン / β-グルコガリン / 生合成経路 / 糖転移酵素 / グリコシルトランスフェラーゼ / Eucalyptus camaldulensis / フトモモ科 / アルミニウム耐性 / 酸性土壌 |
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| Outline of Final Research Achievements |
We have recently found a novel role for hydrolyzable tannins as aluminum-detoxifying compounds in roots of Eucalyptus camaldulensis. The objective of this study was to identify the gene of UDP glycosyltransferase [UGT] in E. camaldulensis, which catalyzes the formation of β-glucogallin, the first step of hydrolyzable tannin biosynthesis. In vitro assays showed that the recombinant proteins of the four candidate UGTs (UGT84A25a, 25b, 26a, 26b) synthesized β-glucogallin from UDP-glucose and gallic acid. Genes of the four candidate UGTs were expressed in leaves, stems, and roots of E. camaldulensis. This gene expression pattern was consistent with the distribution of hydrolyzable tannins in these organs. These results suggest that the four UGT enzymes catalyze the synthesis of β-glucogallin in E. camaldulensis.
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