Noninvasive method for detecting an invasive longicorn beetle in cherry trees
| Project/Area Number |
15K07500
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Forest science
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| Research Institution | Forest Research and Management Organization |
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Principal Investigator |
Shoda-Kagaya Etsuko 国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (70353729)
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| Research Collaborator |
Tamura Shigeaki
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | クビアカツヤカミキリ / 外来生物 / DNA / PCR / 侵略的外来種 / Aromia bungii / サクラ / モモ / ウメ / バラ科 / カミキリムシ |
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| Outline of Final Research Achievements |
Frass suspected to have emitted by invasive beetle Aromia bungii from cherry trees were collected from seven locations. DNA was extracted using some molecular experiment kits. Mitochondrial DNA CO1 region was amplified with universal primers. Some kits were failed to extract DNA from frass. With an adequate method, we can successfully amplified 95% samples. Frass from none damaging area were shown to have emitted by other native longicorn beetles. We designed internal primers which are specific to A. bungii and the molecular method to identify A. bungii invasion using frass from a damaged Rosaceae trees was developed.
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Report
(4 results)
Research Products
(15 results)
