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Noninvasive method for detecting an invasive longicorn beetle in cherry trees

Research Project

Project/Area Number 15K07500
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Forest science
Research InstitutionForest Research and Management Organization

Principal Investigator

Shoda-Kagaya Etsuko  国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (70353729)

Research Collaborator Tamura Shigeaki  
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsクビアカツヤカミキリ / 外来生物 / DNA / PCR / 侵略的外来種 / Aromia bungii / サクラ / モモ / ウメ / バラ科 / カミキリムシ
Outline of Final Research Achievements

Frass suspected to have emitted by invasive beetle Aromia bungii from cherry trees were collected from seven locations. DNA was extracted using some molecular experiment kits. Mitochondrial DNA CO1 region was amplified with universal primers. Some kits were failed to extract DNA from frass. With an adequate method, we can successfully amplified 95% samples. Frass from none damaging area were shown to have emitted by other native longicorn beetles. We designed internal primers which are specific to A. bungii and the molecular method to identify A. bungii invasion using frass from a damaged Rosaceae trees was developed.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (15 results)

All 2018 2017 2016 2015

All Journal Article (5 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (10 results) (of which Invited: 1 results)

  • [Journal Article] サクラ・ウメ・モモ等バラ科樹木を加害するクビアカツヤカミキリAromia bungiiの侵入とその防除法2018

    • Author(s)
      加賀谷悦子
    • Journal Title

      樹木医学研究

      Volume: 22 Pages: 68-72

    • NAID

      130007989757

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 人工飼料を用いたクビアカツヤカミキリ室内飼育個体の発育2018

    • Author(s)
      浦野忠久、加賀谷悦子
    • Journal Title

      関東森林研究

      Volume: 69

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] クビアカツヤカミキリAromia bungii (コウチュウ目:カミキリムシ科)飼育個体の寿命と生涯産卵数2017

    • Author(s)
      浦野忠久、加賀谷悦子
    • Journal Title

      関東森林研究

      Volume: 68

    • Related Report
      2016 Research-status Report
  • [Journal Article] Ecology of the Mountain Pine Beetle <i>Dendroctonus ponderosae</i> (Coleoptera: Scolytidae) and Its Associated Microorganisms: For a Risk Assessment of Its Invasion into Japan2016

    • Author(s)
      加賀谷悦子, 上田明良, 升屋勇人, 神崎菜摘
    • Journal Title

      Japanese Journal of Applied Entomology and Zoology

      Volume: 60 Issue: 2 Pages: 77-86

    • DOI

      10.1303/jjaez.2016.77

    • NAID

      130005164687

    • ISSN
      0021-4914, 1347-6068
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 群馬県館林市・東京都福生市で発生が確認されたサクラ・ウメ等を加害する外来種クビアカツヤカミキリ2015

    • Author(s)
      桐山哲、岩田隆太郎、加賀谷悦子
    • Journal Title

      植物防疫

      Volume: 69(12) Pages: 31-33

    • NAID

      40020670279

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] クビアカツヤカミキリの侵入と被害の拡散2018

    • Author(s)
      加賀谷悦子、浦野忠久
    • Organizer
      日本応用動物昆虫学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] クビアカツヤカミキリが日本に来た:5年間で起こったこと2018

    • Author(s)
      加賀谷悦子
    • Organizer
      日本森林学会森林昆虫談話会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 樹液流出が著しいサクラ樹幹内におけるクビアカツヤカミキリ幼虫の生存2018

    • Author(s)
      加賀谷悦子
    • Organizer
      日本森林学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] クビアカツヤカミキリの合成フェロモントラップで捕獲された昆虫類の組成2018

    • Author(s)
      松本剛史、所雅彦、加賀谷悦子
    • Organizer
      日本森林学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 侵入発見から5年経過した埼玉県草加市・八潮市の外来種クビアカツヤカミキリに対する防除活動と現状2017

    • Author(s)
      桐山哲、加納正行、岩田隆太郎、加賀谷悦子
    • Organizer
      日本昆虫学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 外来種クビアカツヤカミキリ成虫に対する揮発性雄性・集合フェロモンと糖酢液を併用した複合誘引物の誘引性の検証2017

    • Author(s)
      桐山哲、所雅彦、深谷緑、中山雄飛、滝久智、加賀谷悦子、岩田隆太郎
    • Organizer
      日本環境動物昆虫学会年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 東京都あきる野市におけるサクラ等の外来害虫 クビアカツヤカミキリ(Aromia bungii)のフェロモンを用いた誘引試験の結果2017

    • Author(s)
      所雅彦、松本剛史、高梨琢磨、滝久智、浦野忠久、加賀谷悦子
    • Organizer
      関東森林学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 侵入害虫クビアカツヤカミキリの被害状況と防除対策2017

    • Author(s)
      加賀谷悦子
    • Organizer
      第32回報農会シンポジウム 植物保護ハイビジョン-2017
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] サクラ等の外来害虫クビアカツヤカミキリの成虫ルアートラップによる誘引試験2017

    • Author(s)
      衣浦晴生、山本優一、城塚可奈子、所雅彦、加賀谷悦子
    • Organizer
      樹木医学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] クビアカツヤカミキリAromia bungii(コウチュウ目:カミキリムシ科)飼育個体の生存と繁殖2016

    • Author(s)
      浦野忠久、加賀谷悦子
    • Organizer
      第6回関東森林学会大会
    • Place of Presentation
      千葉市民会館(千葉県千葉市)
    • Year and Date
      2016-10-18
    • Related Report
      2016 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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